Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats

Tripterygium wilfordii multiglucoside (TWM) is a fat-soluble extract from a Chinese herb T. wilfordii, that’s used in treating rheumatoid arthritis, nephrotic syndrome and other skin diseases. Triptolide (TP) is a major active component in TWM. However, clinical applications of TWM are limited by i...

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Autores principales:	Liu Xiaojie, Hu Cong, Li Hongwei, Wu Linjing, Xiong Yinhua, Tang Xilan, Deng Siyu
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	fatty acids tripterygium wilfordii multiglucoside triptolide metabolic profile liver injury Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/95faa14c38104a1a99c9c21998084c48
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spelling	oai:doaj.org-article:95faa14c38104a1a99c9c21998084c482021-12-05T14:10:40ZMetabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats2391-541210.1515/biol-2021-0016https://doaj.org/article/95faa14c38104a1a99c9c21998084c482021-02-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0016https://doaj.org/toc/2391-5412Tripterygium wilfordii multiglucoside (TWM) is a fat-soluble extract from a Chinese herb T. wilfordii, that’s used in treating rheumatoid arthritis, nephrotic syndrome and other skin diseases. Triptolide (TP) is a major active component in TWM. However, clinical applications of TWM are limited by its various toxicities especially hepatotoxicity. In recent studies, it has been reported that drug-induced liver injury (DILI) could induce the disorder of lipid metabolism in animals. Hence, this study focuses on the metabolic profile of fatty acids in TWM- and TP-induced liver-injured rats. In serum and liver tissue, 16 free and 16 esterified fatty acids were measured by gas chromatography coupled with mass spectrometry. Metabolic profile of serum fatty acids in rats with liver injury was identified by multivariate statistical analysis. The fatty acid levels in the serum of TWM- and TP-treated rats significantly decreased, whereas those in the liver tissue of TWM- and TP-treated rats obviously increased when compared with the vehicle-treated rats. Four free fatty acids were identified as candidate biomarkers of TWM- and TP-induced liver injury. Therefore, the targeted metabolomic method may be used as a complementary approach for DILI diagnosis in clinic.Liu XiaojieHu CongLi HongweiWu LinjingXiong YinhuaTang XilanDeng SiyuDe Gruyterarticlefatty acidstripterygium wilfordii multiglucosidetriptolidemetabolic profileliver injuryBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 184-197 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fatty acids tripterygium wilfordii multiglucoside triptolide metabolic profile liver injury Biology (General) QH301-705.5
spellingShingle	fatty acids tripterygium wilfordii multiglucoside triptolide metabolic profile liver injury Biology (General) QH301-705.5 Liu Xiaojie Hu Cong Li Hongwei Wu Linjing Xiong Yinhua Tang Xilan Deng Siyu Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
description	Tripterygium wilfordii multiglucoside (TWM) is a fat-soluble extract from a Chinese herb T. wilfordii, that’s used in treating rheumatoid arthritis, nephrotic syndrome and other skin diseases. Triptolide (TP) is a major active component in TWM. However, clinical applications of TWM are limited by its various toxicities especially hepatotoxicity. In recent studies, it has been reported that drug-induced liver injury (DILI) could induce the disorder of lipid metabolism in animals. Hence, this study focuses on the metabolic profile of fatty acids in TWM- and TP-induced liver-injured rats. In serum and liver tissue, 16 free and 16 esterified fatty acids were measured by gas chromatography coupled with mass spectrometry. Metabolic profile of serum fatty acids in rats with liver injury was identified by multivariate statistical analysis. The fatty acid levels in the serum of TWM- and TP-treated rats significantly decreased, whereas those in the liver tissue of TWM- and TP-treated rats obviously increased when compared with the vehicle-treated rats. Four free fatty acids were identified as candidate biomarkers of TWM- and TP-induced liver injury. Therefore, the targeted metabolomic method may be used as a complementary approach for DILI diagnosis in clinic.
format	article
author	Liu Xiaojie Hu Cong Li Hongwei Wu Linjing Xiong Yinhua Tang Xilan Deng Siyu
author_facet	Liu Xiaojie Hu Cong Li Hongwei Wu Linjing Xiong Yinhua Tang Xilan Deng Siyu
author_sort	Liu Xiaojie
title	Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
title_short	Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
title_full	Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
title_fullStr	Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
title_full_unstemmed	Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
title_sort	metabolic profiling of fatty acids in tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/95faa14c38104a1a99c9c21998084c48
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Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats

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