Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection

Abstract MicroRNAs are extremely promising candidates for early cancer diagnosis and prognosis. The levels of circulating microRNAs provide valuable information about cancer disease at its early stages. However, the levels of microRNAs that need to be detected are extremely low and difficult to disc...

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Autores principales:	Serife Ustuner, Mark A. Lindsay, Pedro Estrela
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95fbb1c7b0b44883879e15aa07344ff0
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spelling	oai:doaj.org-article:95fbb1c7b0b44883879e15aa07344ff02021-12-02T17:13:18ZPre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection10.1038/s41598-021-99145-82045-2322https://doaj.org/article/95fbb1c7b0b44883879e15aa07344ff02021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99145-8https://doaj.org/toc/2045-2322Abstract MicroRNAs are extremely promising candidates for early cancer diagnosis and prognosis. The levels of circulating microRNAs provide valuable information about cancer disease at its early stages. However, the levels of microRNAs that need to be detected are extremely low and difficult to discriminate from a large pool of oligonucleotides. There is the need for accurate, rapid and sensitive detection methodologies for detection of microRNAs. We developed electrochemical impedance spectroscopy peptide nucleic acid (PNA)-based sensors that can detect miRNAs in diluted serum with a limit of detection of 0.38 fM. In order to further improve the accuracy and reliability of the sensors, we developed an assay using magnetic beads for simple and rapid fishing of target microRNAs from solution and its pre-concentration prior to electrochemical detection. Our methodology utilizes magnetic beads for the capture of the target microRNA from solution and brings the concentrated sample to the sensor surface. We modify the magnetic beads with locked nucleic acids (LNA), which have high affinity and specificity to their complementary microRNA sequence. The separated and concentrated microRNA is then detected using the PNA-based sensors. By exposing the sensing electrodes only to the captured microRNAs, interferences from other nucleotides or biomolecules from the sample are eliminated.Serife UstunerMark A. LindsayPedro EstrelaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Serife Ustuner Mark A. Lindsay Pedro Estrela Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection
description	Abstract MicroRNAs are extremely promising candidates for early cancer diagnosis and prognosis. The levels of circulating microRNAs provide valuable information about cancer disease at its early stages. However, the levels of microRNAs that need to be detected are extremely low and difficult to discriminate from a large pool of oligonucleotides. There is the need for accurate, rapid and sensitive detection methodologies for detection of microRNAs. We developed electrochemical impedance spectroscopy peptide nucleic acid (PNA)-based sensors that can detect miRNAs in diluted serum with a limit of detection of 0.38 fM. In order to further improve the accuracy and reliability of the sensors, we developed an assay using magnetic beads for simple and rapid fishing of target microRNAs from solution and its pre-concentration prior to electrochemical detection. Our methodology utilizes magnetic beads for the capture of the target microRNA from solution and brings the concentrated sample to the sensor surface. We modify the magnetic beads with locked nucleic acids (LNA), which have high affinity and specificity to their complementary microRNA sequence. The separated and concentrated microRNA is then detected using the PNA-based sensors. By exposing the sensing electrodes only to the captured microRNAs, interferences from other nucleotides or biomolecules from the sample are eliminated.
format	article
author	Serife Ustuner Mark A. Lindsay Pedro Estrela
author_facet	Serife Ustuner Mark A. Lindsay Pedro Estrela
author_sort	Serife Ustuner
title	Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection
title_short	Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection
title_full	Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection
title_fullStr	Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection
title_full_unstemmed	Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection
title_sort	pre-concentration of micrornas by lna-modified magnetic beads for enhancement of electrochemical detection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95fbb1c7b0b44883879e15aa07344ff0
work_keys_str_mv	AT serifeustuner preconcentrationofmicrornasbylnamodifiedmagneticbeadsforenhancementofelectrochemicaldetection AT markalindsay preconcentrationofmicrornasbylnamodifiedmagneticbeadsforenhancementofelectrochemicaldetection AT pedroestrela preconcentrationofmicrornasbylnamodifiedmagneticbeadsforenhancementofelectrochemicaldetection
_version_	1718381332286930944

Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection

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