Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

C Lara-Cruz,1 JE Jiménez-Salazar,1 E Ramón-Gallegos,2 P Damian-Matsumura,1 N Batina3 1Department of Biology of Reproduction, Metropolitan Autonomous University, 2Department of Morphology, National School of Biological Sciences, National Polytechnic Institute, 3Department of Ch...

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Autores principales:	Lara-Cruz C, Jiménez-Salazar JE, Ramón-Gallegos E, Damian-Matsumura P, Batina N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Gold nanoparticles AuNPs membrane roughness Atomic Force Microscopy Confocal Laser Scanning Microscopy breast cancer. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/95fe7f3239ce4c3cabaa5ec389f2a90e
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spelling	oai:doaj.org-article:95fe7f3239ce4c3cabaa5ec389f2a90e2021-12-02T07:46:33ZIncreasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles1178-2013https://doaj.org/article/95fe7f3239ce4c3cabaa5ec389f2a90e2016-10-01T00:00:00Zhttps://www.dovepress.com/increasing-roughness-of-the-human-breast-cancer-cell-membrane-through--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013C Lara-Cruz,1 JE Jiménez-Salazar,1 E Ramón-Gallegos,2 P Damian-Matsumura,1 N Batina3 1Department of Biology of Reproduction, Metropolitan Autonomous University, 2Department of Morphology, National School of Biological Sciences, National Polytechnic Institute, 3Department of Chemistry, Nanotechnology and Molecular Engineering Laboratory, Metropolitan Autonomous University, Mexico City, Mexico Abstract: Gold nanoparticles (AuNPs) have been proposed for use in the treatment of different types of cancer, including breast cancer. At present, neither the mechanisms of AuNP interaction with the plasma membrane surface and their delivery and intracellular distribution in cancer cells nor their effect on the plasma membrane so as to allow cell incorporation of larger amounts of AuNPs is known. The objective of this work was to study the interaction of bare 20 nm diameter AuNPs with the plasma membrane of human MCF-7 breast cancer cells, as well as their uptake, intracellular distribution, and induction of changes on the cell surface roughness. The dynamics of intracellular incorporation and the distribution of AuNPs were observed by confocal laser scanning microscopy. Changes in roughness were monitored in synchronized MCF-7 cells by atomic force microscopy high-resolution imaging at 6 hour intervals for 24 hours during a single cell cycle. The results show that bare AuNPs are capable of emitting fluorescence at 626 nm, without the need for a fluorescent biomarker, which allows monitoring their uptake and intracellular distribution until they reach the nucleus. These results are correlated with changes in cell roughness, which significantly increases at 12 hours of incubation with AuNPs, when compared with control cells. The obtained data provide bases to understand molecular processes of the use of AuNPs in the treatment of different diseases, mainly breast cancer. Keywords: gold nanoparticles uptake, MCF-7 cells, membrane roughness, atomic force microscopy, confocal laser scanning microscopyLara-Cruz CJiménez-Salazar JERamón-Gallegos EDamian-Matsumura PBatina NDove Medical PressarticleGold nanoparticlesAuNPsmembrane roughnessAtomic Force MicroscopyConfocal Laser Scanning Microscopybreast cancer.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5149-5161 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold nanoparticles AuNPs membrane roughness Atomic Force Microscopy Confocal Laser Scanning Microscopy breast cancer. Medicine (General) R5-920
spellingShingle	Gold nanoparticles AuNPs membrane roughness Atomic Force Microscopy Confocal Laser Scanning Microscopy breast cancer. Medicine (General) R5-920 Lara-Cruz C Jiménez-Salazar JE Ramón-Gallegos E Damian-Matsumura P Batina N Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
description	C Lara-Cruz,1 JE Jiménez-Salazar,1 E Ramón-Gallegos,2 P Damian-Matsumura,1 N Batina3 1Department of Biology of Reproduction, Metropolitan Autonomous University, 2Department of Morphology, National School of Biological Sciences, National Polytechnic Institute, 3Department of Chemistry, Nanotechnology and Molecular Engineering Laboratory, Metropolitan Autonomous University, Mexico City, Mexico Abstract: Gold nanoparticles (AuNPs) have been proposed for use in the treatment of different types of cancer, including breast cancer. At present, neither the mechanisms of AuNP interaction with the plasma membrane surface and their delivery and intracellular distribution in cancer cells nor their effect on the plasma membrane so as to allow cell incorporation of larger amounts of AuNPs is known. The objective of this work was to study the interaction of bare 20 nm diameter AuNPs with the plasma membrane of human MCF-7 breast cancer cells, as well as their uptake, intracellular distribution, and induction of changes on the cell surface roughness. The dynamics of intracellular incorporation and the distribution of AuNPs were observed by confocal laser scanning microscopy. Changes in roughness were monitored in synchronized MCF-7 cells by atomic force microscopy high-resolution imaging at 6 hour intervals for 24 hours during a single cell cycle. The results show that bare AuNPs are capable of emitting fluorescence at 626 nm, without the need for a fluorescent biomarker, which allows monitoring their uptake and intracellular distribution until they reach the nucleus. These results are correlated with changes in cell roughness, which significantly increases at 12 hours of incubation with AuNPs, when compared with control cells. The obtained data provide bases to understand molecular processes of the use of AuNPs in the treatment of different diseases, mainly breast cancer. Keywords: gold nanoparticles uptake, MCF-7 cells, membrane roughness, atomic force microscopy, confocal laser scanning microscopy
format	article
author	Lara-Cruz C Jiménez-Salazar JE Ramón-Gallegos E Damian-Matsumura P Batina N
author_facet	Lara-Cruz C Jiménez-Salazar JE Ramón-Gallegos E Damian-Matsumura P Batina N
author_sort	Lara-Cruz C
title	Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
title_short	Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
title_full	Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
title_fullStr	Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
title_full_unstemmed	Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
title_sort	increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/95fe7f3239ce4c3cabaa5ec389f2a90e
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Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

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