Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed

Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. T...

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Autores principales:	Florian J. Mayer, Franz Ratzinger, Ralf L.J. Schmidt, Georg Greiner, Olfert Landt, Alexander Am Ende, Victor M. Corman, Nicole Perkmann-Nagele, Thomas Watkins-Riedel, Dagmar Petermann, Karoline Abadir, Josef Zweimüller-Mayer, Robert Strassl
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	covid-19 sars-cov-2 coronavirus Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/960182eca75642e183f6a2fb60575241
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id	oai:doaj.org-article:960182eca75642e183f6a2fb60575241
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spelling	oai:doaj.org-article:960182eca75642e183f6a2fb605752412021-11-17T14:21:58ZDevelopment of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed2150-55942150-560810.1080/21505594.2020.1798041https://doaj.org/article/960182eca75642e183f6a2fb605752412020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1798041https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84–97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.Florian J. MayerFranz RatzingerRalf L.J. SchmidtGeorg GreinerOlfert LandtAlexander Am EndeVictor M. CormanNicole Perkmann-NageleThomas Watkins-RiedelDagmar PetermannKaroline AbadirJosef Zweimüller-MayerRobert StrasslTaylor & Francis Grouparticlecovid-19sars-cov-2coronavirusInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 964-967 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	covid-19 sars-cov-2 coronavirus Infectious and parasitic diseases RC109-216
spellingShingle	covid-19 sars-cov-2 coronavirus Infectious and parasitic diseases RC109-216 Florian J. Mayer Franz Ratzinger Ralf L.J. Schmidt Georg Greiner Olfert Landt Alexander Am Ende Victor M. Corman Nicole Perkmann-Nagele Thomas Watkins-Riedel Dagmar Petermann Karoline Abadir Josef Zweimüller-Mayer Robert Strassl Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
description	Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84–97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
format	article
author	Florian J. Mayer Franz Ratzinger Ralf L.J. Schmidt Georg Greiner Olfert Landt Alexander Am Ende Victor M. Corman Nicole Perkmann-Nagele Thomas Watkins-Riedel Dagmar Petermann Karoline Abadir Josef Zweimüller-Mayer Robert Strassl
author_facet	Florian J. Mayer Franz Ratzinger Ralf L.J. Schmidt Georg Greiner Olfert Landt Alexander Am Ende Victor M. Corman Nicole Perkmann-Nagele Thomas Watkins-Riedel Dagmar Petermann Karoline Abadir Josef Zweimüller-Mayer Robert Strassl
author_sort	Florian J. Mayer
title	Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_short	Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_full	Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_fullStr	Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_full_unstemmed	Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_sort	development of a fully automated high throughput pcr for the detection of sars-cov-2: the need for speed
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/960182eca75642e183f6a2fb60575241
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Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed

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