Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis.
Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however,...
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2021
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oai:doaj.org-article:961087cf739e4ebf9fcb30ff8f3a887b2021-12-02T20:02:52ZSingle-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis.1553-73901553-740410.1371/journal.pgen.1009728https://doaj.org/article/961087cf739e4ebf9fcb30ff8f3a887b2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009728https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however, the timing and degree of the resulting transcriptional suppression is difficult to separate from global meiotic downregulation of each chromosome. To address this, we analyzed testis single-cell RNA-sequencing (scRNA-seq) data from two Drosophila melanogaster strains. We found evidence that the X chromosome is equally transcriptionally active as autosomes in somatic and pre-meiotic cells, and less transcriptionally active than autosomes in meiotic and post-meiotic cells. In cells experiencing dosage compensation, close proximity to MSL (male-specific lethal) chromatin entry sites (CES) correlates with increased X chromosome transcription. We found low or undetectable levels of germline expression of most msl genes, mle, roX1 and roX2 via scRNA-seq and RNA-FISH, and no evidence of germline nuclear roX1/2 localization. Our results suggest that, although dosage compensation occurs in somatic and pre-meiotic germ cells in Drosophila testis, there might be non-canonical factors involved in the dosage compensation mechanism. The single-cell expression patterns and enrichment statistics of detected genes can be explored interactively in our database: https://zhao.labapps.rockefeller.edu/gene-expr/.Evan WittZhantao ShaoChun HuHenry M KrauseLi ZhaoPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009728 (2021) |
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Genetics QH426-470 Evan Witt Zhantao Shao Chun Hu Henry M Krause Li Zhao Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis. |
description |
Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however, the timing and degree of the resulting transcriptional suppression is difficult to separate from global meiotic downregulation of each chromosome. To address this, we analyzed testis single-cell RNA-sequencing (scRNA-seq) data from two Drosophila melanogaster strains. We found evidence that the X chromosome is equally transcriptionally active as autosomes in somatic and pre-meiotic cells, and less transcriptionally active than autosomes in meiotic and post-meiotic cells. In cells experiencing dosage compensation, close proximity to MSL (male-specific lethal) chromatin entry sites (CES) correlates with increased X chromosome transcription. We found low or undetectable levels of germline expression of most msl genes, mle, roX1 and roX2 via scRNA-seq and RNA-FISH, and no evidence of germline nuclear roX1/2 localization. Our results suggest that, although dosage compensation occurs in somatic and pre-meiotic germ cells in Drosophila testis, there might be non-canonical factors involved in the dosage compensation mechanism. The single-cell expression patterns and enrichment statistics of detected genes can be explored interactively in our database: https://zhao.labapps.rockefeller.edu/gene-expr/. |
format |
article |
author |
Evan Witt Zhantao Shao Chun Hu Henry M Krause Li Zhao |
author_facet |
Evan Witt Zhantao Shao Chun Hu Henry M Krause Li Zhao |
author_sort |
Evan Witt |
title |
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis. |
title_short |
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis. |
title_full |
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis. |
title_fullStr |
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis. |
title_full_unstemmed |
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis. |
title_sort |
single-cell rna-sequencing reveals pre-meiotic x-chromosome dosage compensation in drosophila testis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/961087cf739e4ebf9fcb30ff8f3a887b |
work_keys_str_mv |
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