Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Boran Cheng,1,* Zhaobo He,2,* Libo Zhao,2,* Yuan Fang,1 Yuanyuan Chen,1 Rongxiang He,2 Fangfang Chen,1 Haibin Song,1 Yuliang Deng,2 Xingzhong Zhao,2 Bin Xiong1 1Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cheng BR, He ZB, Zhao LB, Fang Y, Chen YY, He RX, Chen FF, Song HB, Deng YL, Zhao XZ, Xiong B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/96222cd057dc4345afef631e1ede2642
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:96222cd057dc4345afef631e1ede2642
record_format	dspace
spelling	oai:doaj.org-article:96222cd057dc4345afef631e1ede26422021-12-02T00:37:09ZTransparent, biocompatible nanostructured surfaces for cancer cell capture and culture1178-2013https://doaj.org/article/96222cd057dc4345afef631e1ede26422014-05-01T00:00:00Zhttp://www.dovepress.com/transparent-biocompatible-nanostructured-surfaces-for-cancer-cell-capt-a16950https://doaj.org/toc/1178-2013 Boran Cheng,1,* Zhaobo He,2,* Libo Zhao,2,* Yuan Fang,1 Yuanyuan Chen,1 Rongxiang He,2 Fangfang Chen,1 Haibin Song,1 Yuliang Deng,2 Xingzhong Zhao,2 Bin Xiong1 1Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People’s Republic of China; 2Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People’s Republic of China *These authors contributed equally to this work Abstract: Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies. Keywords: cell capture, cell culture, nanofilms, hydroxyapatite/chitosanCheng BRHe ZBZhao LBFang YChen YYHe RXChen FFSong HBDeng YLZhao XZXiong BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2569-2580 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cheng BR He ZB Zhao LB Fang Y Chen YY He RX Chen FF Song HB Deng YL Zhao XZ Xiong B Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
description	Boran Cheng,1,* Zhaobo He,2,* Libo Zhao,2,* Yuan Fang,1 Yuanyuan Chen,1 Rongxiang He,2 Fangfang Chen,1 Haibin Song,1 Yuliang Deng,2 Xingzhong Zhao,2 Bin Xiong1 1Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, People’s Republic of China; 2Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, Hubei, People’s Republic of China *These authors contributed equally to this work Abstract: Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies. Keywords: cell capture, cell culture, nanofilms, hydroxyapatite/chitosan
format	article
author	Cheng BR He ZB Zhao LB Fang Y Chen YY He RX Chen FF Song HB Deng YL Zhao XZ Xiong B
author_facet	Cheng BR He ZB Zhao LB Fang Y Chen YY He RX Chen FF Song HB Deng YL Zhao XZ Xiong B
author_sort	Cheng BR
title	Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_short	Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_full	Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_fullStr	Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_full_unstemmed	Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_sort	transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/96222cd057dc4345afef631e1ede2642
work_keys_str_mv	AT chengbr transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT hezb transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT zhaolb transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT fangy transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT chenyy transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT herx transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT chenff transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT songhb transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT dengyl transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT zhaoxz transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture AT xiongb transparentbiocompatiblenanostructuredsurfacesforcancercellcaptureandculture
_version_	1718403632857088000

Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Ejemplares similares