Optimization of microfluidic biosensor efficiency by means of fluid flow engineering

Abstract Binding reaction kinetics of analyte-ligand at the level of a sensitive membrane into a microchannel of a biosensor has been limited by the formation of the boundary diffusion layer. Therefore, the response time increases and affects the overall performance of a biosensor. In the present wo...

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Autores principales:	Marwa Selmi, Mohamed Hichem Gazzah, Hafedh Belmabrouk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/962349a8448f4a43b18c530fd2b4875e
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spelling	oai:doaj.org-article:962349a8448f4a43b18c530fd2b4875e2021-12-02T11:52:28ZOptimization of microfluidic biosensor efficiency by means of fluid flow engineering10.1038/s41598-017-06204-02045-2322https://doaj.org/article/962349a8448f4a43b18c530fd2b4875e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06204-0https://doaj.org/toc/2045-2322Abstract Binding reaction kinetics of analyte-ligand at the level of a sensitive membrane into a microchannel of a biosensor has been limited by the formation of the boundary diffusion layer. Therefore, the response time increases and affects the overall performance of a biosensor. In the present work, we develop an approach to engineer fluid streams into a complex configuration in order to improve the binding efficiency. We investigate numerically the flow deformations around a parallelepiped with square cross-section inside the microfluidic channel and exploit these deformations to simulate the analyte transport to the sensitive membrane and enhance both association and dissociation processes. The effect of several parameters on the binding reaction is provided such as: the obstacle location from the inlet of the microchannel, the average flow velocity, and the inlet analyte concentration. The optimal position of the obstacle is determined. An appropriate choice of the inlet flow velocity and inlet analyte concentration may reduce significantly the response time.Marwa SelmiMohamed Hichem GazzahHafedh BelmabroukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marwa Selmi Mohamed Hichem Gazzah Hafedh Belmabrouk Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
description	Abstract Binding reaction kinetics of analyte-ligand at the level of a sensitive membrane into a microchannel of a biosensor has been limited by the formation of the boundary diffusion layer. Therefore, the response time increases and affects the overall performance of a biosensor. In the present work, we develop an approach to engineer fluid streams into a complex configuration in order to improve the binding efficiency. We investigate numerically the flow deformations around a parallelepiped with square cross-section inside the microfluidic channel and exploit these deformations to simulate the analyte transport to the sensitive membrane and enhance both association and dissociation processes. The effect of several parameters on the binding reaction is provided such as: the obstacle location from the inlet of the microchannel, the average flow velocity, and the inlet analyte concentration. The optimal position of the obstacle is determined. An appropriate choice of the inlet flow velocity and inlet analyte concentration may reduce significantly the response time.
format	article
author	Marwa Selmi Mohamed Hichem Gazzah Hafedh Belmabrouk
author_facet	Marwa Selmi Mohamed Hichem Gazzah Hafedh Belmabrouk
author_sort	Marwa Selmi
title	Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
title_short	Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
title_full	Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
title_fullStr	Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
title_full_unstemmed	Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
title_sort	optimization of microfluidic biosensor efficiency by means of fluid flow engineering
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/962349a8448f4a43b18c530fd2b4875e
work_keys_str_mv	AT marwaselmi optimizationofmicrofluidicbiosensorefficiencybymeansoffluidflowengineering AT mohamedhichemgazzah optimizationofmicrofluidicbiosensorefficiencybymeansoffluidflowengineering AT hafedhbelmabrouk optimizationofmicrofluidicbiosensorefficiencybymeansoffluidflowengineering
_version_	1718395058988777472

Optimization of microfluidic biosensor efficiency by means of fluid flow engineering

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