Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.

The capacity of gamma-herpesviruses to establish lifelong infections is dependent on the expression of genome maintenance proteins (GMPs) that tether the viral episomes to cellular chromatin and allow their persistence in latently infected proliferating cells. Here we have characterized the chromati...

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Autores principales:	Nouman Mughal, Giuseppe Coppotelli, Simone Callegari, Stefano Gastaldello, Maria G Masucci
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96285ff024c34565ba97f20c1608a929
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spelling	oai:doaj.org-article:96285ff024c34565ba97f20c1608a9292021-11-18T07:46:39ZInteraction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.1932-620310.1371/journal.pone.0062783https://doaj.org/article/96285ff024c34565ba97f20c1608a9292013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667520/?tool=EBIhttps://doaj.org/toc/1932-6203The capacity of gamma-herpesviruses to establish lifelong infections is dependent on the expression of genome maintenance proteins (GMPs) that tether the viral episomes to cellular chromatin and allow their persistence in latently infected proliferating cells. Here we have characterized the chromatin interaction of GMPs encoded by viruses belonging to the genera Lymphocryptovirus (LCV) and Rhadinovirus (RHV). We found that, in addition to a similar diffuse nuclear localization and comparable detergent resistant interaction with chromatin in transfected cells, all GMPs shared the capacity to promote the decondensation of heterochromatin in the A03-1 reporter cell line. They differed, however, in their mobility measured by fluorescence recovery after photobleaching (FRAP), and in the capacity to recruit accessory molecules required for the chromatin remodeling function. While the AT-hook containing GMPs of LCVs were highly mobile, a great variability was observed among GMPs encoded by RHV, ranging from virtually immobile to significantly reduced mobility compared to LCV GMPs. Only the RHV GMPs recruited the bromo- and extra terminal domain (BET) proteins BRD2 and BRD4 to the site of chromatin remodeling. These findings suggest that differences in the mode of interaction with cellular chromatin may underlie different strategies adopted by these viruses for reprogramming of the host cells during latency.Nouman MughalGiuseppe CoppotelliSimone CallegariStefano GastaldelloMaria G MasucciPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62783 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nouman Mughal Giuseppe Coppotelli Simone Callegari Stefano Gastaldello Maria G Masucci Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
description	The capacity of gamma-herpesviruses to establish lifelong infections is dependent on the expression of genome maintenance proteins (GMPs) that tether the viral episomes to cellular chromatin and allow their persistence in latently infected proliferating cells. Here we have characterized the chromatin interaction of GMPs encoded by viruses belonging to the genera Lymphocryptovirus (LCV) and Rhadinovirus (RHV). We found that, in addition to a similar diffuse nuclear localization and comparable detergent resistant interaction with chromatin in transfected cells, all GMPs shared the capacity to promote the decondensation of heterochromatin in the A03-1 reporter cell line. They differed, however, in their mobility measured by fluorescence recovery after photobleaching (FRAP), and in the capacity to recruit accessory molecules required for the chromatin remodeling function. While the AT-hook containing GMPs of LCVs were highly mobile, a great variability was observed among GMPs encoded by RHV, ranging from virtually immobile to significantly reduced mobility compared to LCV GMPs. Only the RHV GMPs recruited the bromo- and extra terminal domain (BET) proteins BRD2 and BRD4 to the site of chromatin remodeling. These findings suggest that differences in the mode of interaction with cellular chromatin may underlie different strategies adopted by these viruses for reprogramming of the host cells during latency.
format	article
author	Nouman Mughal Giuseppe Coppotelli Simone Callegari Stefano Gastaldello Maria G Masucci
author_facet	Nouman Mughal Giuseppe Coppotelli Simone Callegari Stefano Gastaldello Maria G Masucci
author_sort	Nouman Mughal
title	Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
title_short	Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
title_full	Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
title_fullStr	Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
title_full_unstemmed	Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
title_sort	interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/96285ff024c34565ba97f20c1608a929
work_keys_str_mv	AT noumanmughal interactionofgammaherpesvirusgenomemaintenanceproteinswithcellularchromatin AT giuseppecoppotelli interactionofgammaherpesvirusgenomemaintenanceproteinswithcellularchromatin AT simonecallegari interactionofgammaherpesvirusgenomemaintenanceproteinswithcellularchromatin AT stefanogastaldello interactionofgammaherpesvirusgenomemaintenanceproteinswithcellularchromatin AT mariagmasucci interactionofgammaherpesvirusgenomemaintenanceproteinswithcellularchromatin
_version_	1718422988281348096

Interaction of gamma-herpesvirus genome maintenance proteins with cellular chromatin.

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