PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI

Nowadays tuberculosis is considered one of the most dangerous infectious diseases occurring everywhere, and it remains a cause of death of millions of people around the world. According to the World Health Organization data, in 2013 tuberculosis caused more than 9 million cases worldwide and about 1...

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Autores principales:	I. V. Dukhovlinov, E. A. Fedorova, O. A. Dobrovolskaya, E. G. Bogomolova, E. N. Chernyaeva, R. I. Al-Shekhadat, A. S. Simbirtsev
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2016
Materias:	recombinant tb10.4 vaccine tuberculosis producing strain cell culture purifying method Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/9628a34d3f914b3e8f8a6b74ce257900
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spelling	oai:doaj.org-article:9628a34d3f914b3e8f8a6b74ce2579002021-11-22T07:09:47ZPRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI2220-76192313-739810.15789/2220-7619-2015-4-315-322https://doaj.org/article/9628a34d3f914b3e8f8a6b74ce2579002016-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/348https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Nowadays tuberculosis is considered one of the most dangerous infectious diseases occurring everywhere, and it remains a cause of death of millions of people around the world. According to the World Health Organization data, in 2013 tuberculosis caused more than 9 million cases worldwide and about 1.5 million of infected people died. The causative agent of tuberculosis in most cases is Mycobacterium tuberculosis. But sometimes it can be Mycobacterium bovis or Mycobacterium africanum. Mainly as a result of infection, a bacterial infection affects the lungs, but the disease may develop in other organs and tissues. Now for the prevention of tuberculosis vaccination of newborns with attenuated vaccine BCG is widely used. The production of this vaccine is cheap and it is safe to use. Thus today, vaccination is the primary means of prevention of tuberculosis. However dubious efficacy and a number of side effects observed after vaccination, makes the scientific community to develop new effective methods for the treatment of tuberculosis. One of the ways to develop new vaccines against tuberculosis is to provide a subunit vaccine based on recombinant proteins. Advantages of subunit vaccines are that the preparation containing the purified protein is stable and secure, its chemical properties are known, it does not contain additional proteins and nucleic acids, which could cause undesirable effects in the human body. One of the most promising antigens for use as components in new vaccines is considered a low molecular weight secreted protein TB10.4. TB10.4 protein is recognized at an early stage of tuberculous infection and contributes to the proliferation of lymphocytes responsible for the production of IFNγ. TB10.4 protein also possesses an adjuvant effect when administered in combination with mycobacterial proteins. Given these properties, the recombinant protein TB10.4 can be used to generate new candidate vaccines against tuberculosis. During the study was created high-yield E. coli strain, which produces the recombinant protein TB10.4, selected the optimal protocol of induction of the gene encoding the protein. The protein was purified using metal affinity chromatography. The purity of the final preparation reached 98%.I. V. DukhovlinovE. A. FedorovaO. A. DobrovolskayaE. G. BogomolovaE. N. ChernyaevaR. I. Al-ShekhadatA. S. SimbirtsevSankt-Peterburg : NIIÈM imeni Pasteraarticlerecombinant tb10.4vaccinetuberculosisproducing straincell culturepurifying methodInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 5, Iss 4, Pp 315-322 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	recombinant tb10.4 vaccine tuberculosis producing strain cell culture purifying method Infectious and parasitic diseases RC109-216
spellingShingle	recombinant tb10.4 vaccine tuberculosis producing strain cell culture purifying method Infectious and parasitic diseases RC109-216 I. V. Dukhovlinov E. A. Fedorova O. A. Dobrovolskaya E. G. Bogomolova E. N. Chernyaeva R. I. Al-Shekhadat A. S. Simbirtsev PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI
description	Nowadays tuberculosis is considered one of the most dangerous infectious diseases occurring everywhere, and it remains a cause of death of millions of people around the world. According to the World Health Organization data, in 2013 tuberculosis caused more than 9 million cases worldwide and about 1.5 million of infected people died. The causative agent of tuberculosis in most cases is Mycobacterium tuberculosis. But sometimes it can be Mycobacterium bovis or Mycobacterium africanum. Mainly as a result of infection, a bacterial infection affects the lungs, but the disease may develop in other organs and tissues. Now for the prevention of tuberculosis vaccination of newborns with attenuated vaccine BCG is widely used. The production of this vaccine is cheap and it is safe to use. Thus today, vaccination is the primary means of prevention of tuberculosis. However dubious efficacy and a number of side effects observed after vaccination, makes the scientific community to develop new effective methods for the treatment of tuberculosis. One of the ways to develop new vaccines against tuberculosis is to provide a subunit vaccine based on recombinant proteins. Advantages of subunit vaccines are that the preparation containing the purified protein is stable and secure, its chemical properties are known, it does not contain additional proteins and nucleic acids, which could cause undesirable effects in the human body. One of the most promising antigens for use as components in new vaccines is considered a low molecular weight secreted protein TB10.4. TB10.4 protein is recognized at an early stage of tuberculous infection and contributes to the proliferation of lymphocytes responsible for the production of IFNγ. TB10.4 protein also possesses an adjuvant effect when administered in combination with mycobacterial proteins. Given these properties, the recombinant protein TB10.4 can be used to generate new candidate vaccines against tuberculosis. During the study was created high-yield E. coli strain, which produces the recombinant protein TB10.4, selected the optimal protocol of induction of the gene encoding the protein. The protein was purified using metal affinity chromatography. The purity of the final preparation reached 98%.
format	article
author	I. V. Dukhovlinov E. A. Fedorova O. A. Dobrovolskaya E. G. Bogomolova E. N. Chernyaeva R. I. Al-Shekhadat A. S. Simbirtsev
author_facet	I. V. Dukhovlinov E. A. Fedorova O. A. Dobrovolskaya E. G. Bogomolova E. N. Chernyaeva R. I. Al-Shekhadat A. S. Simbirtsev
author_sort	I. V. Dukhovlinov
title	PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI
title_short	PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI
title_full	PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI
title_fullStr	PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI
title_full_unstemmed	PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI
title_sort	production of mycobacterium tuberculosis tb10.4 recombinant protein in escherichia coli
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2016
url	https://doaj.org/article/9628a34d3f914b3e8f8a6b74ce257900
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PRODUCTION OF MYCOBACTERIUM TUBERCULOSIS TB10.4 RECOMBINANT PROTEIN IN ESCHERICHIA COLI

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