Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells

Saud Alarifi,1 Daoud Ali,1 Al Omar Suliman Y,2 Maqusood Ahamed,3 Maqsood A Siddiqui,2 Abdulaziz A Al-Khedhairy21Cell and Molecular Laboratory, Department of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia; 2Faculty of Science, Department of Zoology, King Saud University, Riya...

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Autores principales:	Alarifi S, Ali D, Suliman Y AO, Ahamed M,Siddiqui MA, Al-Khedhairy AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9628d3ce556b4135ac751be52b0a624e
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spelling	oai:doaj.org-article:9628d3ce556b4135ac751be52b0a624e2021-12-02T00:40:26ZOxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells1176-91141178-2013https://doaj.org/article/9628d3ce556b4135ac751be52b0a624e2013-01-01T00:00:00Zhttp://www.dovepress.com/oxidative-stress-contributes-to-cobalt-oxide-nanoparticles-induced-cyt-a11890https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Saud Alarifi,1 Daoud Ali,1 Al Omar Suliman Y,2 Maqusood Ahamed,3 Maqsood A Siddiqui,2 Abdulaziz A Al-Khedhairy21Cell and Molecular Laboratory, Department of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia; 2Faculty of Science, Department of Zoology, King Saud University, Riyadh, Saudi Arabia; 3King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi ArabiaBackground: Cobalt oxide nanoparticles (Co3O4NPs) are increasingly recognized for their utility in biological applications, magnetic resonance imaging, and drug delivery. However, little is known about the toxicity of Co3O4NPs in human cells.Methods: We investigated the possible mechanisms of genotoxicity induced by Co3O4NPs in human hepatocarcinoma (HepG2) cells. Cell viability, reactive oxygen species (ROS), glutathione, thiobarbituric acid reactive substance, apoptosis, and DNA damage were assessed in HepG2 cells after Co3O4NPs and Co2+ exposure.Results: Co3O4NPs elicited a significant (P &lt; 0.01) reduction in glutathione with a concomitant increase in lipid hydroperoxide, ROS generation, superoxide dismutase, and catalase activity after 24- and 48-hour exposure. Co3O4NPs had a mild cytotoxic effect in HepG2 cells; however, it induced ROS and oxidative stress, leading to DNA damage, a probable mechanism of genotoxicity. The comet assay showed a statistically significant (P &lt; 0.01) dose- and time-related increase in DNA damage for Co3O4NPs, whereas Co2+ induced less change than Co3O4NPs but significantly more than control.Conclusion: Our results demonstrated that Co3O4NPs induced cytotoxicity and genotoxicity in HepG2 cells through ROS and oxidative stress.Keywords: cobalt oxide nanoparticles, HepG2 cells, cytotoxicity, oxidative stress, DNA damageAlarifi SAli DSuliman Y AOAhamed M,Siddiqui MAAl-Khedhairy AADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 189-199 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Alarifi S Ali D Suliman Y AO Ahamed M,Siddiqui MA Al-Khedhairy AA Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells
description	Saud Alarifi,1 Daoud Ali,1 Al Omar Suliman Y,2 Maqusood Ahamed,3 Maqsood A Siddiqui,2 Abdulaziz A Al-Khedhairy21Cell and Molecular Laboratory, Department of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia; 2Faculty of Science, Department of Zoology, King Saud University, Riyadh, Saudi Arabia; 3King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi ArabiaBackground: Cobalt oxide nanoparticles (Co3O4NPs) are increasingly recognized for their utility in biological applications, magnetic resonance imaging, and drug delivery. However, little is known about the toxicity of Co3O4NPs in human cells.Methods: We investigated the possible mechanisms of genotoxicity induced by Co3O4NPs in human hepatocarcinoma (HepG2) cells. Cell viability, reactive oxygen species (ROS), glutathione, thiobarbituric acid reactive substance, apoptosis, and DNA damage were assessed in HepG2 cells after Co3O4NPs and Co2+ exposure.Results: Co3O4NPs elicited a significant (P &lt; 0.01) reduction in glutathione with a concomitant increase in lipid hydroperoxide, ROS generation, superoxide dismutase, and catalase activity after 24- and 48-hour exposure. Co3O4NPs had a mild cytotoxic effect in HepG2 cells; however, it induced ROS and oxidative stress, leading to DNA damage, a probable mechanism of genotoxicity. The comet assay showed a statistically significant (P &lt; 0.01) dose- and time-related increase in DNA damage for Co3O4NPs, whereas Co2+ induced less change than Co3O4NPs but significantly more than control.Conclusion: Our results demonstrated that Co3O4NPs induced cytotoxicity and genotoxicity in HepG2 cells through ROS and oxidative stress.Keywords: cobalt oxide nanoparticles, HepG2 cells, cytotoxicity, oxidative stress, DNA damage
format	article
author	Alarifi S Ali D Suliman Y AO Ahamed M,Siddiqui MA Al-Khedhairy AA
author_facet	Alarifi S Ali D Suliman Y AO Ahamed M,Siddiqui MA Al-Khedhairy AA
author_sort	Alarifi S
title	Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells
title_short	Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells
title_full	Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells
title_fullStr	Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells
title_full_unstemmed	Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells
title_sort	oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and dna damage in human hepatocarcinoma cells
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/9628d3ce556b4135ac751be52b0a624e
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Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells

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