A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.

Non-enveloped viruses penetrate host membranes to infect cells. A cell-based assay was used to probe the endoplasmic reticulum (ER)-to-cytosol membrane transport of the non-enveloped SV40. We found that, upon ER arrival, SV40 is released into the lumen and undergoes sequential disulfide bond disrupt...

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Autores principales:	Takamasa Inoue, Billy Tsai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/962b6071a809416c8cf2fe122e6a7d0a
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spelling	oai:doaj.org-article:962b6071a809416c8cf2fe122e6a7d0a2021-11-18T06:03:24ZA large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.1553-73661553-737410.1371/journal.ppat.1002037https://doaj.org/article/962b6071a809416c8cf2fe122e6a7d0a2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21589906/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Non-enveloped viruses penetrate host membranes to infect cells. A cell-based assay was used to probe the endoplasmic reticulum (ER)-to-cytosol membrane transport of the non-enveloped SV40. We found that, upon ER arrival, SV40 is released into the lumen and undergoes sequential disulfide bond disruptions to reach the cytosol. However, despite these ER-dependent conformational changes, SV40 crosses the ER membrane as a large and intact particle consisting of the VP1 coat, the internal components VP2, VP3, and the genome. This large particle subsequently disassembles in the cytosol. Mutant virus and inhibitor studies demonstrate VP3 and likely the viral genome, as well as cellular proteasome, control ER-to-cytosol transport. Our results identify the sequence of events, as well as virus and host components, that regulate ER membrane penetration. They also suggest that the ER membrane supports passage of a large particle, potentially through either a sizeable protein-conducting channel or the lipid bilayer.Takamasa InoueBilly TsaiPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 5, p e1002037 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Takamasa Inoue Billy Tsai A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
description	Non-enveloped viruses penetrate host membranes to infect cells. A cell-based assay was used to probe the endoplasmic reticulum (ER)-to-cytosol membrane transport of the non-enveloped SV40. We found that, upon ER arrival, SV40 is released into the lumen and undergoes sequential disulfide bond disruptions to reach the cytosol. However, despite these ER-dependent conformational changes, SV40 crosses the ER membrane as a large and intact particle consisting of the VP1 coat, the internal components VP2, VP3, and the genome. This large particle subsequently disassembles in the cytosol. Mutant virus and inhibitor studies demonstrate VP3 and likely the viral genome, as well as cellular proteasome, control ER-to-cytosol transport. Our results identify the sequence of events, as well as virus and host components, that regulate ER membrane penetration. They also suggest that the ER membrane supports passage of a large particle, potentially through either a sizeable protein-conducting channel or the lipid bilayer.
format	article
author	Takamasa Inoue Billy Tsai
author_facet	Takamasa Inoue Billy Tsai
author_sort	Takamasa Inoue
title	A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
title_short	A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
title_full	A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
title_fullStr	A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
title_full_unstemmed	A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
title_sort	large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/962b6071a809416c8cf2fe122e6a7d0a
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A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol.

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