CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport

Abstract Hepatic fibrosis is caused by exaggerated wound healing response to chronic injury, which eventually leads to hepatic cirrhosis. Differentiation of hepatic stellate cells (HSCs) to myofibroblast-like cells by inflammatory cytokines is the critical step in fibrosis. This step is accompanied...

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Autores principales:	Shotaro Tomoishi, Shinichi Fukushima, Kentaro Shinohara, Toshiaki Katada, Kota Saito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/962d23b9ba8644049160c4dea9506310
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spelling	oai:doaj.org-article:962d23b9ba8644049160c4dea95063102021-12-02T15:05:39ZCREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport10.1038/s41598-017-08703-62045-2322https://doaj.org/article/962d23b9ba8644049160c4dea95063102017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08703-6https://doaj.org/toc/2045-2322Abstract Hepatic fibrosis is caused by exaggerated wound healing response to chronic injury, which eventually leads to hepatic cirrhosis. Differentiation of hepatic stellate cells (HSCs) to myofibroblast-like cells by inflammatory cytokines is the critical step in fibrosis. This step is accompanied by enlargement of the endoplasmic reticulum (ER) and Golgi apparatus, suggesting that protein synthesis and secretion are augmented in the activated HSCs. However, the process of rearrangement of secretory organelles and their functions remain to be fully elucidated. Here, we revealed that differentiation alters early secretory gene expression. We observed significant isoform-specific upregulation of the inner coat protein complex II (COPII) components, Sec23A and Sec24D, via the transmembrane bZIP transcription factor, CREB3L2/BBF2H7, during HSC activation. Moreover, knockdown of these components abrogated the activation, suggesting that Sec23A/Sec24D-mediated ER to Golgi trafficking is required for HSC activation.Shotaro TomoishiShinichi FukushimaKentaro ShinoharaToshiaki KatadaKota SaitoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shotaro Tomoishi Shinichi Fukushima Kentaro Shinohara Toshiaki Katada Kota Saito CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport
description	Abstract Hepatic fibrosis is caused by exaggerated wound healing response to chronic injury, which eventually leads to hepatic cirrhosis. Differentiation of hepatic stellate cells (HSCs) to myofibroblast-like cells by inflammatory cytokines is the critical step in fibrosis. This step is accompanied by enlargement of the endoplasmic reticulum (ER) and Golgi apparatus, suggesting that protein synthesis and secretion are augmented in the activated HSCs. However, the process of rearrangement of secretory organelles and their functions remain to be fully elucidated. Here, we revealed that differentiation alters early secretory gene expression. We observed significant isoform-specific upregulation of the inner coat protein complex II (COPII) components, Sec23A and Sec24D, via the transmembrane bZIP transcription factor, CREB3L2/BBF2H7, during HSC activation. Moreover, knockdown of these components abrogated the activation, suggesting that Sec23A/Sec24D-mediated ER to Golgi trafficking is required for HSC activation.
format	article
author	Shotaro Tomoishi Shinichi Fukushima Kentaro Shinohara Toshiaki Katada Kota Saito
author_facet	Shotaro Tomoishi Shinichi Fukushima Kentaro Shinohara Toshiaki Katada Kota Saito
author_sort	Shotaro Tomoishi
title	CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport
title_short	CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport
title_full	CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport
title_fullStr	CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport
title_full_unstemmed	CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport
title_sort	creb3l2-mediated expression of sec23a/sec24d is involved in hepatic stellate cell activation through er-golgi transport
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/962d23b9ba8644049160c4dea9506310
work_keys_str_mv	AT shotarotomoishi creb3l2mediatedexpressionofsec23asec24disinvolvedinhepaticstellatecellactivationthroughergolgitransport AT shinichifukushima creb3l2mediatedexpressionofsec23asec24disinvolvedinhepaticstellatecellactivationthroughergolgitransport AT kentaroshinohara creb3l2mediatedexpressionofsec23asec24disinvolvedinhepaticstellatecellactivationthroughergolgitransport AT toshiakikatada creb3l2mediatedexpressionofsec23asec24disinvolvedinhepaticstellatecellactivationthroughergolgitransport AT kotasaito creb3l2mediatedexpressionofsec23asec24disinvolvedinhepaticstellatecellactivationthroughergolgitransport
_version_	1718388782349156352

CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport

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