Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide

Abstract To enhance the reducing sugar yield in enzymatic hydrolysis, various factors (NaOH concentration, solid content and pre-treatment time) that affect the pre-treatment process were investigated and evaluated based on the reducing sugar yield of the subsequent enzymatic hydrolysis. The enzymat...

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Autores principales:	Zhiquan Wang, Suqing Wu, Chunzhen Fan, Xiangyong Zheng, Wei Zhang, Deyi Wu, Xinze Wang, Hainan Kong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96340ed51bfd4714a5a0ccc512af70d3
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spelling	oai:doaj.org-article:96340ed51bfd4714a5a0ccc512af70d32021-12-05T12:13:42ZOptimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide10.1038/s41598-021-02693-22045-2322https://doaj.org/article/96340ed51bfd4714a5a0ccc512af70d32021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02693-2https://doaj.org/toc/2045-2322Abstract To enhance the reducing sugar yield in enzymatic hydrolysis, various factors (NaOH concentration, solid content and pre-treatment time) that affect the pre-treatment process were investigated and evaluated based on the reducing sugar yield of the subsequent enzymatic hydrolysis. The enzymatic hydrolysis was based on the cellulase from Trichoderma reesi ATCC 26921, the optimum NaOH pre-treatment conditions were an NaOH concentration of 1.0% (w/w), a solid content of 5.0% (w/v) and a pre-treatment time of 60 min. Various parameters that affect the enzymatic hydrolysis of wheat straw, including the solid content, enzyme loading, pH and hydrolysis time, were investigated and optimized through a Box–Behnken design and response surface methodology. The predicted optimum conditions for enzymatic hydrolysis were a solid content of 8.0% (w/v), an enzyme loading of 35 FPU/g substrate, a temperature of 50 °C, a pH of 5.3 and a hydrolysis time of 96 h. The experimental result showed that the maximum reducing sugar yield was 60.73% (53.35% higher than the wheat straw without NaOH pre-treatment), which is in accordance with the predicted conditions.Zhiquan WangSuqing WuChunzhen FanXiangyong ZhengWei ZhangDeyi WuXinze WangHainan KongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiquan Wang Suqing Wu Chunzhen Fan Xiangyong Zheng Wei Zhang Deyi Wu Xinze Wang Hainan Kong Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
description	Abstract To enhance the reducing sugar yield in enzymatic hydrolysis, various factors (NaOH concentration, solid content and pre-treatment time) that affect the pre-treatment process were investigated and evaluated based on the reducing sugar yield of the subsequent enzymatic hydrolysis. The enzymatic hydrolysis was based on the cellulase from Trichoderma reesi ATCC 26921, the optimum NaOH pre-treatment conditions were an NaOH concentration of 1.0% (w/w), a solid content of 5.0% (w/v) and a pre-treatment time of 60 min. Various parameters that affect the enzymatic hydrolysis of wheat straw, including the solid content, enzyme loading, pH and hydrolysis time, were investigated and optimized through a Box–Behnken design and response surface methodology. The predicted optimum conditions for enzymatic hydrolysis were a solid content of 8.0% (w/v), an enzyme loading of 35 FPU/g substrate, a temperature of 50 °C, a pH of 5.3 and a hydrolysis time of 96 h. The experimental result showed that the maximum reducing sugar yield was 60.73% (53.35% higher than the wheat straw without NaOH pre-treatment), which is in accordance with the predicted conditions.
format	article
author	Zhiquan Wang Suqing Wu Chunzhen Fan Xiangyong Zheng Wei Zhang Deyi Wu Xinze Wang Hainan Kong
author_facet	Zhiquan Wang Suqing Wu Chunzhen Fan Xiangyong Zheng Wei Zhang Deyi Wu Xinze Wang Hainan Kong
author_sort	Zhiquan Wang
title	Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
title_short	Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
title_full	Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
title_fullStr	Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
title_full_unstemmed	Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
title_sort	optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/96340ed51bfd4714a5a0ccc512af70d3
work_keys_str_mv	AT zhiquanwang optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT suqingwu optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT chunzhenfan optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT xiangyongzheng optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT weizhang optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT deyiwu optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT xinzewang optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide AT hainankong optimisationofenzymaticsaccharificationofwheatstrawpretreatedwithsodiumhydroxide
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Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide

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