Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications

The use of biomaterials in medicine is becoming increasingly important. One of the main concerns is the foreign body associated infection caused by direct microbial contamination or clinical infections. The bacterial biofilm formation on biomaterials depends on their surface properties. Therefore, s...

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Autores principales:	Teske Michael, Kießlich Tina, Fischer Julia, Bahl Hubert, Wulf Katharina, Eickner Thomas, Grabow Niels, Illner Sabine
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2020
Materias:	biofilm inhibition cardiovascular application surface immobilization plla papain edc/nhs Medicine R
Acceso en línea:	https://doaj.org/article/963da6f80de24def94ceef9cd450409f
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spelling	oai:doaj.org-article:963da6f80de24def94ceef9cd450409f2021-12-05T14:10:42ZImmobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications2364-550410.1515/cdbme-2020-3044https://doaj.org/article/963da6f80de24def94ceef9cd450409f2020-09-01T00:00:00Zhttps://doi.org/10.1515/cdbme-2020-3044https://doaj.org/toc/2364-5504The use of biomaterials in medicine is becoming increasingly important. One of the main concerns is the foreign body associated infection caused by direct microbial contamination or clinical infections. The bacterial biofilm formation on biomaterials depends on their surface properties. Therefore, several anti-adhesive surface modifications were developed. Nevertheless, the demand for antimicrobial agents that prevent bacterial colonisation is still largely unmet. The immobilization of active antimicrobial agents, such as antibacterial peptides or enzymes, offers a potential approach to achieve long-lasting effectiveness. In this investigation, the hydrolytic enzyme papain with its published antibacterial activity was covalently immobilized on the well-established biodegradable biomaterial poly-L-lactic acid (PLLA). For the characterization of the enzymes on the PLLA surfaces, the protein content and enzyme activity were determined. A biofilm assay was performed to test the effect of the papain-modified PLLA samples on the biofilm-forming bacterial strain Clostridioides difficile, one of the most frequently occurring human nosocomial pathogens. The investigated hydrolytic enzyme papain could be immobilized by coupling via the crosslinker EDC to the PLLA surface. Detection was performed by determination of the amount of protein and the reduced biofilm growth after 24 h and 72 h compared to the reference.Teske MichaelKießlich TinaFischer JuliaBahl HubertWulf KatharinaEickner ThomasGrabow NielsIllner SabineDe Gruyterarticlebiofilm inhibitioncardiovascular applicationsurface immobilizationpllapapainedc/nhsMedicineRENCurrent Directions in Biomedical Engineering, Vol 6, Iss 3, Pp 172-175 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm inhibition cardiovascular application surface immobilization plla papain edc/nhs Medicine R
spellingShingle	biofilm inhibition cardiovascular application surface immobilization plla papain edc/nhs Medicine R Teske Michael Kießlich Tina Fischer Julia Bahl Hubert Wulf Katharina Eickner Thomas Grabow Niels Illner Sabine Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications
description	The use of biomaterials in medicine is becoming increasingly important. One of the main concerns is the foreign body associated infection caused by direct microbial contamination or clinical infections. The bacterial biofilm formation on biomaterials depends on their surface properties. Therefore, several anti-adhesive surface modifications were developed. Nevertheless, the demand for antimicrobial agents that prevent bacterial colonisation is still largely unmet. The immobilization of active antimicrobial agents, such as antibacterial peptides or enzymes, offers a potential approach to achieve long-lasting effectiveness. In this investigation, the hydrolytic enzyme papain with its published antibacterial activity was covalently immobilized on the well-established biodegradable biomaterial poly-L-lactic acid (PLLA). For the characterization of the enzymes on the PLLA surfaces, the protein content and enzyme activity were determined. A biofilm assay was performed to test the effect of the papain-modified PLLA samples on the biofilm-forming bacterial strain Clostridioides difficile, one of the most frequently occurring human nosocomial pathogens. The investigated hydrolytic enzyme papain could be immobilized by coupling via the crosslinker EDC to the PLLA surface. Detection was performed by determination of the amount of protein and the reduced biofilm growth after 24 h and 72 h compared to the reference.
format	article
author	Teske Michael Kießlich Tina Fischer Julia Bahl Hubert Wulf Katharina Eickner Thomas Grabow Niels Illner Sabine
author_facet	Teske Michael Kießlich Tina Fischer Julia Bahl Hubert Wulf Katharina Eickner Thomas Grabow Niels Illner Sabine
author_sort	Teske Michael
title	Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications
title_short	Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications
title_full	Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications
title_fullStr	Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications
title_full_unstemmed	Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications
title_sort	immobilizing hydrolytic active papain on biodegradable plla for biofilm inhibition in cardiovascular applications
publisher	De Gruyter
publishDate	2020
url	https://doaj.org/article/963da6f80de24def94ceef9cd450409f
work_keys_str_mv	AT teskemichael immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT kießlichtina immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT fischerjulia immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT bahlhubert immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT wulfkatharina immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT eicknerthomas immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT grabowniels immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications AT illnersabine immobilizinghydrolyticactivepapainonbiodegradablepllaforbiofilminhibitionincardiovascularapplications
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Immobilizing hydrolytic active Papain on biodegradable PLLA for biofilm inhibition in cardiovascular applications

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