The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation

Abstract The process of myogenesis which operates during skeletal muscle regeneration involves the activation of muscle stem cells, the so-called satellite cells. These then give rise to proliferating progenitors, the myoblasts which subsequently exit the cell cycle and differentiate into committed...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tannaz Norizadeh Abbariki, Zita Gonda, Denise Kemler, Pavel Urbanek, Tabea Wagner, Margarethe Litfin, Zhao-Qi Wang, Peter Herrlich, Olivier Kassel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/963e02f1d9ae4bd6815ed8cea91f78f5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:963e02f1d9ae4bd6815ed8cea91f78f5
record_format	dspace
spelling	oai:doaj.org-article:963e02f1d9ae4bd6815ed8cea91f78f52021-12-02T17:41:26ZThe LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation10.1038/s41598-021-92331-82045-2322https://doaj.org/article/963e02f1d9ae4bd6815ed8cea91f78f52021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92331-8https://doaj.org/toc/2045-2322Abstract The process of myogenesis which operates during skeletal muscle regeneration involves the activation of muscle stem cells, the so-called satellite cells. These then give rise to proliferating progenitors, the myoblasts which subsequently exit the cell cycle and differentiate into committed precursors, the myocytes. Ultimately, the fusion of myocytes leads to myofiber formation. Here we reveal a role for the transcriptional co-regulator nTRIP6, the nuclear isoform of the LIM-domain protein TRIP6, in the temporal control of myogenesis. In an in vitro model of myogenesis, the expression of nTRIP6 is transiently up-regulated at the transition between proliferation and differentiation, whereas that of the cytosolic isoform TRIP6 is not altered. Selectively blocking nTRIP6 function results in accelerated early differentiation followed by deregulated late differentiation and fusion. Thus, the transient increase in nTRIP6 expression appears to prevent premature differentiation. Accordingly, knocking out the Trip6 gene in satellite cells leads to deregulated skeletal muscle regeneration dynamics in the mouse. Thus, dynamic changes in nTRIP6 expression contributes to the temporal control of myogenesis.Tannaz Norizadeh AbbarikiZita GondaDenise KemlerPavel UrbanekTabea WagnerMargarethe LitfinZhao-Qi WangPeter HerrlichOlivier KasselNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tannaz Norizadeh Abbariki Zita Gonda Denise Kemler Pavel Urbanek Tabea Wagner Margarethe Litfin Zhao-Qi Wang Peter Herrlich Olivier Kassel The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation
description	Abstract The process of myogenesis which operates during skeletal muscle regeneration involves the activation of muscle stem cells, the so-called satellite cells. These then give rise to proliferating progenitors, the myoblasts which subsequently exit the cell cycle and differentiate into committed precursors, the myocytes. Ultimately, the fusion of myocytes leads to myofiber formation. Here we reveal a role for the transcriptional co-regulator nTRIP6, the nuclear isoform of the LIM-domain protein TRIP6, in the temporal control of myogenesis. In an in vitro model of myogenesis, the expression of nTRIP6 is transiently up-regulated at the transition between proliferation and differentiation, whereas that of the cytosolic isoform TRIP6 is not altered. Selectively blocking nTRIP6 function results in accelerated early differentiation followed by deregulated late differentiation and fusion. Thus, the transient increase in nTRIP6 expression appears to prevent premature differentiation. Accordingly, knocking out the Trip6 gene in satellite cells leads to deregulated skeletal muscle regeneration dynamics in the mouse. Thus, dynamic changes in nTRIP6 expression contributes to the temporal control of myogenesis.
format	article
author	Tannaz Norizadeh Abbariki Zita Gonda Denise Kemler Pavel Urbanek Tabea Wagner Margarethe Litfin Zhao-Qi Wang Peter Herrlich Olivier Kassel
author_facet	Tannaz Norizadeh Abbariki Zita Gonda Denise Kemler Pavel Urbanek Tabea Wagner Margarethe Litfin Zhao-Qi Wang Peter Herrlich Olivier Kassel
author_sort	Tannaz Norizadeh Abbariki
title	The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation
title_short	The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation
title_full	The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation
title_fullStr	The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation
title_full_unstemmed	The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation
title_sort	lim domain protein ntrip6 modulates the dynamics of myogenic differentiation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/963e02f1d9ae4bd6815ed8cea91f78f5
work_keys_str_mv	AT tannaznorizadehabbariki thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT zitagonda thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT denisekemler thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT pavelurbanek thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT tabeawagner thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT margarethelitfin thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT zhaoqiwang thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT peterherrlich thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT olivierkassel thelimdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT tannaznorizadehabbariki limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT zitagonda limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT denisekemler limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT pavelurbanek limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT tabeawagner limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT margarethelitfin limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT zhaoqiwang limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT peterherrlich limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation AT olivierkassel limdomainproteinntrip6modulatesthedynamicsofmyogenicdifferentiation
_version_	1718379718098550784

The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation

Ejemplares similares