An in vitro method to keep human aortic tissue sections functionally and structurally intact

Abstract The pathophysiology of aortic aneurysms (AA) is far from being understood. One reason for this lack of understanding is basic research being constrained to fixated cells or isolated cell cultures, by which cell-to-cell and cell-to-matrix communications are missed. We present a new, in vitro...

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Autores principales:	Jorn P. Meekel, Menno E. Groeneveld, Natalija Bogunovic, Niels Keekstra, René J. P. Musters, Behrouz Zandieh-Doulabi, Gerard Pals, Dimitra Micha, Hans W. M. Niessen, Arno M. Wiersema, Jur K. Kievit, Arjan W. J. Hoksbergen, Willem Wisselink, Jan D. Blankensteijn, Kak K. Yeung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9643e47c11644d7eb054de8537310ecc
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spelling	oai:doaj.org-article:9643e47c11644d7eb054de8537310ecc2021-12-02T15:09:09ZAn in vitro method to keep human aortic tissue sections functionally and structurally intact10.1038/s41598-018-26549-42045-2322https://doaj.org/article/9643e47c11644d7eb054de8537310ecc2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26549-4https://doaj.org/toc/2045-2322Abstract The pathophysiology of aortic aneurysms (AA) is far from being understood. One reason for this lack of understanding is basic research being constrained to fixated cells or isolated cell cultures, by which cell-to-cell and cell-to-matrix communications are missed. We present a new, in vitro method for extended preservation of aortic wall sections to study pathophysiological processes. Intraoperatively harvested, live aortic specimens were cut into 150 μm sections and cultured. Viability was quantified up to 92 days using immunofluorescence. Cell types were characterized using immunostaining. After 14 days, individual cells of enzymatically digested tissues were examined for cell type and viability. Analysis of AA sections (N = 8) showed a viability of 40% at 7 days and smooth muscle cells, leukocytes, and macrophages were observed. Protocol optimization (N = 4) showed higher stable viability at day 62 and proliferation of new cells at day 92. Digested tissues showed different cell types and a viability up to 75% at day 14. Aortic tissue viability can be preserved until at least 62 days after harvesting. Cultured tissues can be digested into viable single cells for additional techniques. Present protocol provides an appropriate ex vivo setting to discover and study pathways and mechanisms in cultured human aneurysmal aortic tissue.Jorn P. MeekelMenno E. GroeneveldNatalija BogunovicNiels KeekstraRené J. P. MustersBehrouz Zandieh-DoulabiGerard PalsDimitra MichaHans W. M. NiessenArno M. WiersemaJur K. KievitArjan W. J. HoksbergenWillem WisselinkJan D. BlankensteijnKak K. YeungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jorn P. Meekel Menno E. Groeneveld Natalija Bogunovic Niels Keekstra René J. P. Musters Behrouz Zandieh-Doulabi Gerard Pals Dimitra Micha Hans W. M. Niessen Arno M. Wiersema Jur K. Kievit Arjan W. J. Hoksbergen Willem Wisselink Jan D. Blankensteijn Kak K. Yeung An in vitro method to keep human aortic tissue sections functionally and structurally intact
description	Abstract The pathophysiology of aortic aneurysms (AA) is far from being understood. One reason for this lack of understanding is basic research being constrained to fixated cells or isolated cell cultures, by which cell-to-cell and cell-to-matrix communications are missed. We present a new, in vitro method for extended preservation of aortic wall sections to study pathophysiological processes. Intraoperatively harvested, live aortic specimens were cut into 150 μm sections and cultured. Viability was quantified up to 92 days using immunofluorescence. Cell types were characterized using immunostaining. After 14 days, individual cells of enzymatically digested tissues were examined for cell type and viability. Analysis of AA sections (N = 8) showed a viability of 40% at 7 days and smooth muscle cells, leukocytes, and macrophages were observed. Protocol optimization (N = 4) showed higher stable viability at day 62 and proliferation of new cells at day 92. Digested tissues showed different cell types and a viability up to 75% at day 14. Aortic tissue viability can be preserved until at least 62 days after harvesting. Cultured tissues can be digested into viable single cells for additional techniques. Present protocol provides an appropriate ex vivo setting to discover and study pathways and mechanisms in cultured human aneurysmal aortic tissue.
format	article
author	Jorn P. Meekel Menno E. Groeneveld Natalija Bogunovic Niels Keekstra René J. P. Musters Behrouz Zandieh-Doulabi Gerard Pals Dimitra Micha Hans W. M. Niessen Arno M. Wiersema Jur K. Kievit Arjan W. J. Hoksbergen Willem Wisselink Jan D. Blankensteijn Kak K. Yeung
author_facet	Jorn P. Meekel Menno E. Groeneveld Natalija Bogunovic Niels Keekstra René J. P. Musters Behrouz Zandieh-Doulabi Gerard Pals Dimitra Micha Hans W. M. Niessen Arno M. Wiersema Jur K. Kievit Arjan W. J. Hoksbergen Willem Wisselink Jan D. Blankensteijn Kak K. Yeung
author_sort	Jorn P. Meekel
title	An in vitro method to keep human aortic tissue sections functionally and structurally intact
title_short	An in vitro method to keep human aortic tissue sections functionally and structurally intact
title_full	An in vitro method to keep human aortic tissue sections functionally and structurally intact
title_fullStr	An in vitro method to keep human aortic tissue sections functionally and structurally intact
title_full_unstemmed	An in vitro method to keep human aortic tissue sections functionally and structurally intact
title_sort	in vitro method to keep human aortic tissue sections functionally and structurally intact
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9643e47c11644d7eb054de8537310ecc
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An in vitro method to keep human aortic tissue sections functionally and structurally intact

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