Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni

Campylobacter jejuni is a common cause of diarrheal disease worldwide. Human infection typically occurs through the ingestion of contaminated poultry products. We previously demonstrated that an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan on its surface reduced...

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Autores principales:	Harald Nothaft, Maria Elisa Perez-Muñoz, Tianfu Yang, Abarna V. M. Murugan, Michelle Miller, Daniel Kolarich, Graham S. Plastow, Jens Walter, Christine M. Szymanski
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	vaccine N-glycan Campylobacter immune response fecal transplant Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/964a1334ebd14bc9aceaa8ca1591e3d0
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spelling	oai:doaj.org-article:964a1334ebd14bc9aceaa8ca1591e3d02021-11-16T17:32:17ZImproving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni1664-302X10.3389/fmicb.2021.734526https://doaj.org/article/964a1334ebd14bc9aceaa8ca1591e3d02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.734526/fullhttps://doaj.org/toc/1664-302XCampylobacter jejuni is a common cause of diarrheal disease worldwide. Human infection typically occurs through the ingestion of contaminated poultry products. We previously demonstrated that an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan on its surface reduced the Campylobacter load in more than 50% of vaccinated leghorn and broiler birds to undetectable levels (responder birds), whereas the remainder of the animals was still colonized (non-responders). To understand the underlying mechanism, we conducted three vaccination and challenge studies using 135 broiler birds and found a similar responder/non-responder effect. Subsequent genome-wide association studies (GWAS), analyses of bird sex and levels of vaccine-induced IgY responses did not correlate with the responder versus non-responder phenotype. In contrast, antibodies isolated from responder birds displayed a higher Campylobacter-opsonophagocytic activity when compared to antisera from non-responder birds. No differences in the N-glycome of the sera could be detected, although minor changes in IgY glycosylation warrant further investigation. As reported before, the composition of the microbiota, particularly levels of OTU classified as Clostridium spp., Ruminococcaceae and Lachnospiraceae are associated with the response. Transplantation of the cecal microbiota of responder birds into new birds in combination with vaccination resulted in further increases in vaccine-induced antigen-specific IgY responses when compared to birds that did not receive microbiota transplants. Our work suggests that the IgY effector function and microbiota contribute to the efficacy of the E. coli live vaccine, information that could form the basis for the development of improved vaccines targeted at the elimination of C. jejuni from poultry.Harald NothaftMaria Elisa Perez-MuñozTianfu YangAbarna V. M. MuruganMichelle MillerDaniel KolarichDaniel KolarichGraham S. PlastowGraham S. PlastowJens WalterChristine M. SzymanskiChristine M. SzymanskiFrontiers Media S.A.articlevaccineN-glycanCampylobacterimmune responsefecal transplantMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vaccine N-glycan Campylobacter immune response fecal transplant Microbiology QR1-502
spellingShingle	vaccine N-glycan Campylobacter immune response fecal transplant Microbiology QR1-502 Harald Nothaft Maria Elisa Perez-Muñoz Tianfu Yang Abarna V. M. Murugan Michelle Miller Daniel Kolarich Daniel Kolarich Graham S. Plastow Graham S. Plastow Jens Walter Christine M. Szymanski Christine M. Szymanski Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni
description	Campylobacter jejuni is a common cause of diarrheal disease worldwide. Human infection typically occurs through the ingestion of contaminated poultry products. We previously demonstrated that an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan on its surface reduced the Campylobacter load in more than 50% of vaccinated leghorn and broiler birds to undetectable levels (responder birds), whereas the remainder of the animals was still colonized (non-responders). To understand the underlying mechanism, we conducted three vaccination and challenge studies using 135 broiler birds and found a similar responder/non-responder effect. Subsequent genome-wide association studies (GWAS), analyses of bird sex and levels of vaccine-induced IgY responses did not correlate with the responder versus non-responder phenotype. In contrast, antibodies isolated from responder birds displayed a higher Campylobacter-opsonophagocytic activity when compared to antisera from non-responder birds. No differences in the N-glycome of the sera could be detected, although minor changes in IgY glycosylation warrant further investigation. As reported before, the composition of the microbiota, particularly levels of OTU classified as Clostridium spp., Ruminococcaceae and Lachnospiraceae are associated with the response. Transplantation of the cecal microbiota of responder birds into new birds in combination with vaccination resulted in further increases in vaccine-induced antigen-specific IgY responses when compared to birds that did not receive microbiota transplants. Our work suggests that the IgY effector function and microbiota contribute to the efficacy of the E. coli live vaccine, information that could form the basis for the development of improved vaccines targeted at the elimination of C. jejuni from poultry.
format	article
author	Harald Nothaft Maria Elisa Perez-Muñoz Tianfu Yang Abarna V. M. Murugan Michelle Miller Daniel Kolarich Daniel Kolarich Graham S. Plastow Graham S. Plastow Jens Walter Christine M. Szymanski Christine M. Szymanski
author_facet	Harald Nothaft Maria Elisa Perez-Muñoz Tianfu Yang Abarna V. M. Murugan Michelle Miller Daniel Kolarich Daniel Kolarich Graham S. Plastow Graham S. Plastow Jens Walter Christine M. Szymanski Christine M. Szymanski
author_sort	Harald Nothaft
title	Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni
title_short	Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni
title_full	Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni
title_fullStr	Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni
title_full_unstemmed	Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni
title_sort	improving chicken responses to glycoconjugate vaccination against campylobacter jejuni
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/964a1334ebd14bc9aceaa8ca1591e3d0
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Improving Chicken Responses to Glycoconjugate Vaccination Against Campylobacter jejuni

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