circ_0082375 promotes the progression of glioma by regulating Wnt7B

Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member...

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Autores principales:	Meng Xianbing, Tian Hailong, Guo Wenqiang, Wang Zhigang
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	glioma circ_0082375 mir-485-5p wnt7b Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/964a99144cfa455690a5917a513ef17e
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spelling	oai:doaj.org-article:964a99144cfa455690a5917a513ef17e2021-12-05T14:11:05Zcirc_0082375 promotes the progression of glioma by regulating Wnt7B2081-693610.1515/tnsci-2020-0181https://doaj.org/article/964a99144cfa455690a5917a513ef17e2021-11-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2020-0181https://doaj.org/toc/2081-6936Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member 7B (Wnt7B). The overall survival of glioma patients was estimated by the Kaplan–Meier curve. Cell proliferation, apoptosis, invasion, and migration were detected by cell counting kit-8, 5-ethynyl-2 -deoxyuridine (EdU) staining, flow cytometry, and transwell assays, respectively. Glucose level and lactate production were determined using glucose and lactate assay kits. In vitro angiogenesis assay was used to evaluate the angiogenesis of glioma cells. The interaction between microRNA (miR)-485-5p and circ_0082375 or Wnt family member 7B (Wnt7B) was verified by dual-luciferase reporter and RNA immunoprecipitation assays. A xenograft model was used to verify the function of circ_0082375 in vivo. circ_0082375 was upregulated in glioma tissues, and it was closely related to the prognosis of glioma patients. circ_0082375 knockdown suppressed cell proliferation, migration, invasion, angiogenesis, glycolysis, and epithelial-mesenchymal transition (EMT), and promoted cell apoptosis in glioma cells. irc_0082375 was a sponge of miR-485-5p, which directly targeted Wnt7B. Knockdown of circ_0082375 inhibited the malignancy, angiogenesis, and glycolysis of glioma cells in vitro by sponging miR-485-5p. Besides, circ_0082375 knockdown hampered the growth of glioma growth by regulating the miR-485-5p/Wnt7B axis in vivo. Altogether, circ_0082375 regulated miR-485-5p/Wnt7B axis to promote the malignancy, angiogenesis, and glycolysis of glioma cells, thereby contributing to the progression of glioma.Meng XianbingTian HailongGuo WenqiangWang ZhigangDe Gruyterarticlegliomacirc_0082375mir-485-5pwnt7bNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 456-468 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioma circ_0082375 mir-485-5p wnt7b Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	glioma circ_0082375 mir-485-5p wnt7b Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Meng Xianbing Tian Hailong Guo Wenqiang Wang Zhigang circ_0082375 promotes the progression of glioma by regulating Wnt7B
description	Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member 7B (Wnt7B). The overall survival of glioma patients was estimated by the Kaplan–Meier curve. Cell proliferation, apoptosis, invasion, and migration were detected by cell counting kit-8, 5-ethynyl-2 -deoxyuridine (EdU) staining, flow cytometry, and transwell assays, respectively. Glucose level and lactate production were determined using glucose and lactate assay kits. In vitro angiogenesis assay was used to evaluate the angiogenesis of glioma cells. The interaction between microRNA (miR)-485-5p and circ_0082375 or Wnt family member 7B (Wnt7B) was verified by dual-luciferase reporter and RNA immunoprecipitation assays. A xenograft model was used to verify the function of circ_0082375 in vivo. circ_0082375 was upregulated in glioma tissues, and it was closely related to the prognosis of glioma patients. circ_0082375 knockdown suppressed cell proliferation, migration, invasion, angiogenesis, glycolysis, and epithelial-mesenchymal transition (EMT), and promoted cell apoptosis in glioma cells. irc_0082375 was a sponge of miR-485-5p, which directly targeted Wnt7B. Knockdown of circ_0082375 inhibited the malignancy, angiogenesis, and glycolysis of glioma cells in vitro by sponging miR-485-5p. Besides, circ_0082375 knockdown hampered the growth of glioma growth by regulating the miR-485-5p/Wnt7B axis in vivo. Altogether, circ_0082375 regulated miR-485-5p/Wnt7B axis to promote the malignancy, angiogenesis, and glycolysis of glioma cells, thereby contributing to the progression of glioma.
format	article
author	Meng Xianbing Tian Hailong Guo Wenqiang Wang Zhigang
author_facet	Meng Xianbing Tian Hailong Guo Wenqiang Wang Zhigang
author_sort	Meng Xianbing
title	circ_0082375 promotes the progression of glioma by regulating Wnt7B
title_short	circ_0082375 promotes the progression of glioma by regulating Wnt7B
title_full	circ_0082375 promotes the progression of glioma by regulating Wnt7B
title_fullStr	circ_0082375 promotes the progression of glioma by regulating Wnt7B
title_full_unstemmed	circ_0082375 promotes the progression of glioma by regulating Wnt7B
title_sort	circ_0082375 promotes the progression of glioma by regulating wnt7b
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/964a99144cfa455690a5917a513ef17e
work_keys_str_mv	AT mengxianbing circ0082375promotestheprogressionofgliomabyregulatingwnt7b AT tianhailong circ0082375promotestheprogressionofgliomabyregulatingwnt7b AT guowenqiang circ0082375promotestheprogressionofgliomabyregulatingwnt7b AT wangzhigang circ0082375promotestheprogressionofgliomabyregulatingwnt7b
_version_	1718371435953520640

circ_0082375 promotes the progression of glioma by regulating Wnt7B

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