Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
Zhifeng Jiang,1,* Song Chen,2,* Lin Zhang,1,* Jie Shen,1 Ming Zhong2,* 1Department of Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of China; 2Division of Critical Care Medicine, Zhongshan Hospital Fudan University, Sh...
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2021
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| Acceso en línea: | https://doaj.org/article/9653314323d74fba9367e985bd616cd0 |
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| Sumario: | Zhifeng Jiang,1,* Song Chen,2,* Lin Zhang,1,* Jie Shen,1 Ming Zhong2,* 1Department of Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of China; 2Division of Critical Care Medicine, Zhongshan Hospital Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lin ZhangDepartment of Emergency and Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of ChinaEmail linzhang0315@fudan.edu.cnMing ZhongDivision of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of ChinaEmail zhong_ming@fudan.edu.cnBackground: Intestinal ischemia-reperfusion (II/R) injury is a common clinical complication associated with high mortality, for which microRNA (miRNA) drives potentially its pathophysiological progression. MiRNAs regulate different messenger RNAs (mRNAs). However, the regulatory network between miRNAs and mRNAs in intestinal ischemia-reperfusion injury is elusive.Methods: We analyzed the different expression of mRNAs and miRNAs in intestinal tissues from patients from three groups (arterial group (group A), venous group (group V), control group (group C)). Common differentially expressed (Co-DE) miRNAs and differentially expressed mRNAs were acquired via concerned analyses among the three groups. Co-DE mRNAs were shared parts of target mRNAs and differentially expression mRNAs. Cytoscape was employed to construct the regulatory network between miRNAs and mRNAs. Gene Ontology (GO) analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway depicted the functions and potential pathway associated with Co-DE mRNAs. Using the STRING and Cytoscape, we found critical mRNAs in the protein–protein interaction (PPI) network.Results: The miRNA-mRNA network comprised 8 Co-DE miRNAs and 140 Co-DE mRNAs. Of note, 140 Co-DE mRNAs were targets of these 8 miRNAs, and their roles were established through the functional exploration via GO analysis and KEGG analysis. PPI network and Cytoscape revealed COL1A2, THY1, IL10, MMP2, SERPINH1, COL3A1, COL14A1, and P4HA1 as the top 8 key mRNAs.Conclusion: This study has demonstrated a miRNA-mRNA regulatory network in intestinal ischemia-reperfusion injury, and explored the key mRNAs and their potential functions. These findings could provide new insight into prognostic markers and therapeutic targets for patients with intestinal ischemia-reperfusion injury in clinical practice.Keywords: intestinal ischemia-reperfusion injury, microRNA, prognostic marker, bioinformatics |
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