Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis

Zhifeng Jiang,1,&ast; Song Chen,2,&ast; Lin Zhang,1,&ast; Jie Shen,1 Ming Zhong2,&ast; 1Department of Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of China; 2Division of Critical Care Medicine, Zhongshan Hospital Fudan University, Sh...

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Autores principales:	Jiang Z, Chen S, Zhang L, Shen J, Zhong M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	intestinal ischemia-reperfusion injury microrna prognostic marker bioinformatics Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9653314323d74fba9367e985bd616cd0
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spelling	oai:doaj.org-article:9653314323d74fba9367e985bd616cd02021-12-02T15:16:25ZPotentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis1178-7031https://doaj.org/article/9653314323d74fba9367e985bd616cd02021-09-01T00:00:00Zhttps://www.dovepress.com/potentially-functional-microrna-mrna-regulatory-networks-in-intestinal-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Zhifeng Jiang,1,&ast; Song Chen,2,&ast; Lin Zhang,1,&ast; Jie Shen,1 Ming Zhong2,&ast; 1Department of Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of China; 2Division of Critical Care Medicine, Zhongshan Hospital Fudan University, Shanghai, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Lin ZhangDepartment of Emergency and Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of ChinaEmail linzhang0315@fudan.edu.cnMing ZhongDivision of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of ChinaEmail zhong_ming@fudan.edu.cnBackground: Intestinal ischemia-reperfusion (II/R) injury is a common clinical complication associated with high mortality, for which microRNA (miRNA) drives potentially its pathophysiological progression. MiRNAs regulate different messenger RNAs (mRNAs). However, the regulatory network between miRNAs and mRNAs in intestinal ischemia-reperfusion injury is elusive.Methods: We analyzed the different expression of mRNAs and miRNAs in intestinal tissues from patients from three groups (arterial group (group A), venous group (group V), control group (group C)). Common differentially expressed (Co-DE) miRNAs and differentially expressed mRNAs were acquired via concerned analyses among the three groups. Co-DE mRNAs were shared parts of target mRNAs and differentially expression mRNAs. Cytoscape was employed to construct the regulatory network between miRNAs and mRNAs. Gene Ontology (GO) analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway depicted the functions and potential pathway associated with Co-DE mRNAs. Using the STRING and Cytoscape, we found critical mRNAs in the protein–protein interaction (PPI) network.Results: The miRNA-mRNA network comprised 8 Co-DE miRNAs and 140 Co-DE mRNAs. Of note, 140 Co-DE mRNAs were targets of these 8 miRNAs, and their roles were established through the functional exploration via GO analysis and KEGG analysis. PPI network and Cytoscape revealed COL1A2, THY1, IL10, MMP2, SERPINH1, COL3A1, COL14A1, and P4HA1 as the top 8 key mRNAs.Conclusion: This study has demonstrated a miRNA-mRNA regulatory network in intestinal ischemia-reperfusion injury, and explored the key mRNAs and their potential functions. These findings could provide new insight into prognostic markers and therapeutic targets for patients with intestinal ischemia-reperfusion injury in clinical practice.Keywords: intestinal ischemia-reperfusion injury, microRNA, prognostic marker, bioinformaticsJiang ZChen SZhang LShen JZhong MDove Medical Pressarticleintestinal ischemia-reperfusion injurymicrornaprognostic markerbioinformaticsPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 4817-4825 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	intestinal ischemia-reperfusion injury microrna prognostic marker bioinformatics Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	intestinal ischemia-reperfusion injury microrna prognostic marker bioinformatics Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Jiang Z Chen S Zhang L Shen J Zhong M Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
description	Zhifeng Jiang,1,&ast; Song Chen,2,&ast; Lin Zhang,1,&ast; Jie Shen,1 Ming Zhong2,&ast; 1Department of Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of China; 2Division of Critical Care Medicine, Zhongshan Hospital Fudan University, Shanghai, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Lin ZhangDepartment of Emergency and Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, 201508, People’s Republic of ChinaEmail linzhang0315@fudan.edu.cnMing ZhongDivision of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of ChinaEmail zhong_ming@fudan.edu.cnBackground: Intestinal ischemia-reperfusion (II/R) injury is a common clinical complication associated with high mortality, for which microRNA (miRNA) drives potentially its pathophysiological progression. MiRNAs regulate different messenger RNAs (mRNAs). However, the regulatory network between miRNAs and mRNAs in intestinal ischemia-reperfusion injury is elusive.Methods: We analyzed the different expression of mRNAs and miRNAs in intestinal tissues from patients from three groups (arterial group (group A), venous group (group V), control group (group C)). Common differentially expressed (Co-DE) miRNAs and differentially expressed mRNAs were acquired via concerned analyses among the three groups. Co-DE mRNAs were shared parts of target mRNAs and differentially expression mRNAs. Cytoscape was employed to construct the regulatory network between miRNAs and mRNAs. Gene Ontology (GO) analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway depicted the functions and potential pathway associated with Co-DE mRNAs. Using the STRING and Cytoscape, we found critical mRNAs in the protein–protein interaction (PPI) network.Results: The miRNA-mRNA network comprised 8 Co-DE miRNAs and 140 Co-DE mRNAs. Of note, 140 Co-DE mRNAs were targets of these 8 miRNAs, and their roles were established through the functional exploration via GO analysis and KEGG analysis. PPI network and Cytoscape revealed COL1A2, THY1, IL10, MMP2, SERPINH1, COL3A1, COL14A1, and P4HA1 as the top 8 key mRNAs.Conclusion: This study has demonstrated a miRNA-mRNA regulatory network in intestinal ischemia-reperfusion injury, and explored the key mRNAs and their potential functions. These findings could provide new insight into prognostic markers and therapeutic targets for patients with intestinal ischemia-reperfusion injury in clinical practice.Keywords: intestinal ischemia-reperfusion injury, microRNA, prognostic marker, bioinformatics
format	article
author	Jiang Z Chen S Zhang L Shen J Zhong M
author_facet	Jiang Z Chen S Zhang L Shen J Zhong M
author_sort	Jiang Z
title	Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
title_short	Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
title_full	Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
title_fullStr	Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
title_full_unstemmed	Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis
title_sort	potentially functional microrna-mrna regulatory networks in intestinal ischemia-reperfusion injury: a bioinformatics analysis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/9653314323d74fba9367e985bd616cd0
work_keys_str_mv	AT jiangz potentiallyfunctionalmicrornamrnaregulatorynetworksinintestinalischemiareperfusioninjuryabioinformaticsanalysis AT chens potentiallyfunctionalmicrornamrnaregulatorynetworksinintestinalischemiareperfusioninjuryabioinformaticsanalysis AT zhangl potentiallyfunctionalmicrornamrnaregulatorynetworksinintestinalischemiareperfusioninjuryabioinformaticsanalysis AT shenj potentiallyfunctionalmicrornamrnaregulatorynetworksinintestinalischemiareperfusioninjuryabioinformaticsanalysis AT zhongm potentiallyfunctionalmicrornamrnaregulatorynetworksinintestinalischemiareperfusioninjuryabioinformaticsanalysis
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Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis

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