Polyamine metabolism links gut microbiota and testicular dysfunction

Abstract Background Male fertility impaired by exogenous toxins is a serious worldwide issue threatening the health of the new-born and causing infertility. However, the metabolic connection between toxic exposures and testicular dysfunction remains unclear. Results In the present study, the metabol...

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Autores principales:	Qi Zhao, Jian-Feng Huang, Yan Cheng, Man-Yun Dai, Wei-Feng Zhu, Xiu-Wei Yang, Frank J. Gonzalez, Fei Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Testicular dysfunction Gut microbiota Polyamine metabolism Metabolomics Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/96582235b83142678a6f6dedccf72d38
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spelling	oai:doaj.org-article:96582235b83142678a6f6dedccf72d382021-11-14T12:10:18ZPolyamine metabolism links gut microbiota and testicular dysfunction10.1186/s40168-021-01157-z2049-2618https://doaj.org/article/96582235b83142678a6f6dedccf72d382021-11-01T00:00:00Zhttps://doi.org/10.1186/s40168-021-01157-zhttps://doaj.org/toc/2049-2618Abstract Background Male fertility impaired by exogenous toxins is a serious worldwide issue threatening the health of the new-born and causing infertility. However, the metabolic connection between toxic exposures and testicular dysfunction remains unclear. Results In the present study, the metabolic disorder of testicular dysfunction was investigated using triptolide-induced testicular injury in mice. We found that triptolide induced spermine deficiency resulting from disruption of polyamine biosynthesis and uptake in testis, and perturbation of the gut microbiota. Supplementation with exogenous spermine reversed triptolide-induced testicular dysfunction through increasing the expression of genes related to early and late spermatogenic events, as well as increasing the reduced number of offspring. Loss of gut microbiota by antibiotic treatment resulted in depletion of spermine levels in the intestine and potentiation of testicular injury. Testicular dysfunction in triptolide-treated mice was reversed by gut microbial transplantation from untreated mice and supplementation with polyamine-producing Parabacteroides distasonis. The protective effect of spermine during testicular injury was largely dependent on upregulation of heat shock protein 70s (HSP70s) both in vivo and in vitro. Conclusions The present study linked alterations in the gut microbiota to testicular dysfunction through disruption of polyamine metabolism. The diversity and dynamics of the gut microbiota may be considered as a therapeutic option to prevent male infertility. Video AbstractQi ZhaoJian-Feng HuangYan ChengMan-Yun DaiWei-Feng ZhuXiu-Wei YangFrank J. GonzalezFei LiBMCarticleTesticular dysfunctionGut microbiotaPolyamine metabolismMetabolomicsMicrobial ecologyQR100-130ENMicrobiome, Vol 9, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Testicular dysfunction Gut microbiota Polyamine metabolism Metabolomics Microbial ecology QR100-130
spellingShingle	Testicular dysfunction Gut microbiota Polyamine metabolism Metabolomics Microbial ecology QR100-130 Qi Zhao Jian-Feng Huang Yan Cheng Man-Yun Dai Wei-Feng Zhu Xiu-Wei Yang Frank J. Gonzalez Fei Li Polyamine metabolism links gut microbiota and testicular dysfunction
description	Abstract Background Male fertility impaired by exogenous toxins is a serious worldwide issue threatening the health of the new-born and causing infertility. However, the metabolic connection between toxic exposures and testicular dysfunction remains unclear. Results In the present study, the metabolic disorder of testicular dysfunction was investigated using triptolide-induced testicular injury in mice. We found that triptolide induced spermine deficiency resulting from disruption of polyamine biosynthesis and uptake in testis, and perturbation of the gut microbiota. Supplementation with exogenous spermine reversed triptolide-induced testicular dysfunction through increasing the expression of genes related to early and late spermatogenic events, as well as increasing the reduced number of offspring. Loss of gut microbiota by antibiotic treatment resulted in depletion of spermine levels in the intestine and potentiation of testicular injury. Testicular dysfunction in triptolide-treated mice was reversed by gut microbial transplantation from untreated mice and supplementation with polyamine-producing Parabacteroides distasonis. The protective effect of spermine during testicular injury was largely dependent on upregulation of heat shock protein 70s (HSP70s) both in vivo and in vitro. Conclusions The present study linked alterations in the gut microbiota to testicular dysfunction through disruption of polyamine metabolism. The diversity and dynamics of the gut microbiota may be considered as a therapeutic option to prevent male infertility. Video Abstract
format	article
author	Qi Zhao Jian-Feng Huang Yan Cheng Man-Yun Dai Wei-Feng Zhu Xiu-Wei Yang Frank J. Gonzalez Fei Li
author_facet	Qi Zhao Jian-Feng Huang Yan Cheng Man-Yun Dai Wei-Feng Zhu Xiu-Wei Yang Frank J. Gonzalez Fei Li
author_sort	Qi Zhao
title	Polyamine metabolism links gut microbiota and testicular dysfunction
title_short	Polyamine metabolism links gut microbiota and testicular dysfunction
title_full	Polyamine metabolism links gut microbiota and testicular dysfunction
title_fullStr	Polyamine metabolism links gut microbiota and testicular dysfunction
title_full_unstemmed	Polyamine metabolism links gut microbiota and testicular dysfunction
title_sort	polyamine metabolism links gut microbiota and testicular dysfunction
publisher	BMC
publishDate	2021
url	https://doaj.org/article/96582235b83142678a6f6dedccf72d38
work_keys_str_mv	AT qizhao polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT jianfenghuang polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT yancheng polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT manyundai polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT weifengzhu polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT xiuweiyang polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT frankjgonzalez polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction AT feili polyaminemetabolismlinksgutmicrobiotaandtesticulardysfunction
_version_	1718429418615996416

Polyamine metabolism links gut microbiota and testicular dysfunction

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