Comparison of commercial ELISA assays for quantification of corticosterone in serum

Abstract Enzyme-linked immunosorbent assay (ELISA) kits are widely used to quantify corticosterone levels for the assessment of stress in laboratory animals. The aim of this experiment was simply to evaluate if four different and widely used commercial ELISA assays would yield the same or similar va...

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Autores principales:	Anne Marie Kinn Rød, Nina Harkestad, Finn Konow Jellestad, Robert Murison
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9658a880a9c34180bc49a93d6476ea0d
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spelling	oai:doaj.org-article:9658a880a9c34180bc49a93d6476ea0d2021-12-02T16:06:09ZComparison of commercial ELISA assays for quantification of corticosterone in serum10.1038/s41598-017-06006-42045-2322https://doaj.org/article/9658a880a9c34180bc49a93d6476ea0d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06006-4https://doaj.org/toc/2045-2322Abstract Enzyme-linked immunosorbent assay (ELISA) kits are widely used to quantify corticosterone levels for the assessment of stress in laboratory animals. The aim of this experiment was simply to evaluate if four different and widely used commercial ELISA assays would yield the same or similar values of corticosterone in serum samples taken from laboratory rats after the mild stress of being held for sampling blood from the saphenous vein. Trunk blood was sampled from 32 male Wistar rats 30 minutes after this mild stress exposure and analysed with each of four commercial ELISA kits. Both the Arbor Assays and the DRG-4164 kits were significantly higher than the DRG-5186 and the Enzo kits. There were no significant differences between the DRG-5186 and Enzo kits. Overall the correlations between kits were high. In conclusion, the commercial ELISA kits tested in the present experiment yielded different values of total corticosterone in the same serum samples. The precision in determining true values of the corticosterone level is low for these commercial ELISA kits, although they may be used to determine relative differences within studies.Anne Marie Kinn RødNina HarkestadFinn Konow JellestadRobert MurisonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne Marie Kinn Rød Nina Harkestad Finn Konow Jellestad Robert Murison Comparison of commercial ELISA assays for quantification of corticosterone in serum
description	Abstract Enzyme-linked immunosorbent assay (ELISA) kits are widely used to quantify corticosterone levels for the assessment of stress in laboratory animals. The aim of this experiment was simply to evaluate if four different and widely used commercial ELISA assays would yield the same or similar values of corticosterone in serum samples taken from laboratory rats after the mild stress of being held for sampling blood from the saphenous vein. Trunk blood was sampled from 32 male Wistar rats 30 minutes after this mild stress exposure and analysed with each of four commercial ELISA kits. Both the Arbor Assays and the DRG-4164 kits were significantly higher than the DRG-5186 and the Enzo kits. There were no significant differences between the DRG-5186 and Enzo kits. Overall the correlations between kits were high. In conclusion, the commercial ELISA kits tested in the present experiment yielded different values of total corticosterone in the same serum samples. The precision in determining true values of the corticosterone level is low for these commercial ELISA kits, although they may be used to determine relative differences within studies.
format	article
author	Anne Marie Kinn Rød Nina Harkestad Finn Konow Jellestad Robert Murison
author_facet	Anne Marie Kinn Rød Nina Harkestad Finn Konow Jellestad Robert Murison
author_sort	Anne Marie Kinn Rød
title	Comparison of commercial ELISA assays for quantification of corticosterone in serum
title_short	Comparison of commercial ELISA assays for quantification of corticosterone in serum
title_full	Comparison of commercial ELISA assays for quantification of corticosterone in serum
title_fullStr	Comparison of commercial ELISA assays for quantification of corticosterone in serum
title_full_unstemmed	Comparison of commercial ELISA assays for quantification of corticosterone in serum
title_sort	comparison of commercial elisa assays for quantification of corticosterone in serum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9658a880a9c34180bc49a93d6476ea0d
work_keys_str_mv	AT annemariekinnrød comparisonofcommercialelisaassaysforquantificationofcorticosteroneinserum AT ninaharkestad comparisonofcommercialelisaassaysforquantificationofcorticosteroneinserum AT finnkonowjellestad comparisonofcommercialelisaassaysforquantificationofcorticosteroneinserum AT robertmurison comparisonofcommercialelisaassaysforquantificationofcorticosteroneinserum
_version_	1718385103321694208

Comparison of commercial ELISA assays for quantification of corticosterone in serum

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