Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).

<h4>Background</h4>The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most economically important pests in the world, causing serious damage to fruit production. However, lack of genetic information on this organism is an obstacle to understanding the mechanisms behind i...

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Autores principales:	Guang-Mao Shen, Wei Dou, Jin-Zhi Niu, Hong-Bo Jiang, Wen-Jia Yang, Fu-Xian Jia, Fei Hu, Lin Cong, Jin-Jun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/965945920bb546208225ed77833d0cb3
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spelling	oai:doaj.org-article:965945920bb546208225ed77833d0cb32021-11-18T07:32:00ZTranscriptome analysis of the oriental fruit fly (Bactrocera dorsalis).1932-620310.1371/journal.pone.0029127https://doaj.org/article/965945920bb546208225ed77833d0cb32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22195006/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most economically important pests in the world, causing serious damage to fruit production. However, lack of genetic information on this organism is an obstacle to understanding the mechanisms behind its development and its ability to resist insecticides. Analysis of the B. dorsalis transcriptome and its expression profile data is essential to extending the genetic information resources on this species, providing a shortcut that will support studies on B. dorsalis.<h4>Methodology/principal findings</h4>We performed de novo assembly of a transcriptome using short read sequencing technology (Illumina). The results generated 484,628 contigs, 70,640 scaffolds, and 49,804 unigenes. Of those unigenes, 27,455 (55.13%) matched known proteins in the NCBI database, as determined by BLAST search. Clusters of orthologous groups (COG), gene orthology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations were performed to better understand the functions of these unigenes. Genes related to insecticide resistance were analyzed in additional detail. Digital gene expression (DGE) libraries showed differences in gene expression profiles at different developmental stages (eggs, third-instar larvae, pupae, and adults). To confirm the DGE results, the expression profiles of six randomly selected genes were analyzed.<h4>Conclusion/significance</h4>This transcriptome greatly improves our genetic understanding of B. dorsalis and makes a huge number of gene sequences available for further study, including both genes of known importance and genes of unknown function. The DGE data provide comprehensive insight into gene expression profiles at different developmental stages. This facilitates the study of the role of each gene in the developmental process and in insecticide resistance.Guang-Mao ShenWei DouJin-Zhi NiuHong-Bo JiangWen-Jia YangFu-Xian JiaFei HuLin CongJin-Jun WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e29127 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guang-Mao Shen Wei Dou Jin-Zhi Niu Hong-Bo Jiang Wen-Jia Yang Fu-Xian Jia Fei Hu Lin Cong Jin-Jun Wang Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).
description	<h4>Background</h4>The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most economically important pests in the world, causing serious damage to fruit production. However, lack of genetic information on this organism is an obstacle to understanding the mechanisms behind its development and its ability to resist insecticides. Analysis of the B. dorsalis transcriptome and its expression profile data is essential to extending the genetic information resources on this species, providing a shortcut that will support studies on B. dorsalis.<h4>Methodology/principal findings</h4>We performed de novo assembly of a transcriptome using short read sequencing technology (Illumina). The results generated 484,628 contigs, 70,640 scaffolds, and 49,804 unigenes. Of those unigenes, 27,455 (55.13%) matched known proteins in the NCBI database, as determined by BLAST search. Clusters of orthologous groups (COG), gene orthology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations were performed to better understand the functions of these unigenes. Genes related to insecticide resistance were analyzed in additional detail. Digital gene expression (DGE) libraries showed differences in gene expression profiles at different developmental stages (eggs, third-instar larvae, pupae, and adults). To confirm the DGE results, the expression profiles of six randomly selected genes were analyzed.<h4>Conclusion/significance</h4>This transcriptome greatly improves our genetic understanding of B. dorsalis and makes a huge number of gene sequences available for further study, including both genes of known importance and genes of unknown function. The DGE data provide comprehensive insight into gene expression profiles at different developmental stages. This facilitates the study of the role of each gene in the developmental process and in insecticide resistance.
format	article
author	Guang-Mao Shen Wei Dou Jin-Zhi Niu Hong-Bo Jiang Wen-Jia Yang Fu-Xian Jia Fei Hu Lin Cong Jin-Jun Wang
author_facet	Guang-Mao Shen Wei Dou Jin-Zhi Niu Hong-Bo Jiang Wen-Jia Yang Fu-Xian Jia Fei Hu Lin Cong Jin-Jun Wang
author_sort	Guang-Mao Shen
title	Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).
title_short	Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).
title_full	Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).
title_fullStr	Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).
title_full_unstemmed	Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).
title_sort	transcriptome analysis of the oriental fruit fly (bactrocera dorsalis).
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/965945920bb546208225ed77833d0cb3
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Transcriptome analysis of the oriental fruit fly (Bactrocera dorsalis).

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