LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis

Abstract Background miR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU...

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Autores principales:	Caihong Zheng, Shiwei Liu, Zhihong Qin, Xiaoqian Zhang, Yubao Song
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	DLEU1 POF miR-146b-5p Premature ovarian failure Gynecology and obstetrics RG1-991
Acceso en línea:	https://doaj.org/article/966b7ec8588b44d6966f69500f158b75
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spelling	oai:doaj.org-article:966b7ec8588b44d6966f69500f158b752021-11-08T11:04:00ZLncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis10.1186/s13048-021-00905-x1757-2215https://doaj.org/article/966b7ec8588b44d6966f69500f158b752021-11-01T00:00:00Zhttps://doi.org/10.1186/s13048-021-00905-xhttps://doaj.org/toc/1757-2215Abstract Background miR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU1 and miR-146b-5p. Methods Expression of DLEU1 and miR-146b-5p in POF and control ovary tissues was determined by RT-qPCR. The subcellular location of DLEU1 in human KGN cells was analyzed using subcellular fractionation assays. The direct interaction between DLEU1 and miR-146b-5p was analyzed using RNA pull-down assays. The role of DLEU1 in miR-146a expression was analyzed using overexpression assay. Cell proliferation was analyzed using cell apoptosis assay. Results Increased DLEU1 expression and decreased miR-146b-5p expression were observed in POF. DLEU1 directly interacted with MiR-146b-5p and was expressed in both nuclear and cytoplasm samples of KGN cells. In KGN cells, DLEU1 and miR-146b-5p failed to regulate the expression of each other. However, DLEU1 promoted cell apoptosis and reduced the inhibitory effects of miR-146b-5p on cell apoptosis. Conclusions DLEU1 is overexpressed in POF and sponges miR-146b-5p to increase KGN cell apoptosis.Caihong ZhengShiwei LiuZhihong QinXiaoqian ZhangYubao SongBMCarticleDLEU1POFmiR-146b-5pPremature ovarian failureGynecology and obstetricsRG1-991ENJournal of Ovarian Research, Vol 14, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DLEU1 POF miR-146b-5p Premature ovarian failure Gynecology and obstetrics RG1-991
spellingShingle	DLEU1 POF miR-146b-5p Premature ovarian failure Gynecology and obstetrics RG1-991 Caihong Zheng Shiwei Liu Zhihong Qin Xiaoqian Zhang Yubao Song LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis
description	Abstract Background miR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU1 and miR-146b-5p. Methods Expression of DLEU1 and miR-146b-5p in POF and control ovary tissues was determined by RT-qPCR. The subcellular location of DLEU1 in human KGN cells was analyzed using subcellular fractionation assays. The direct interaction between DLEU1 and miR-146b-5p was analyzed using RNA pull-down assays. The role of DLEU1 in miR-146a expression was analyzed using overexpression assay. Cell proliferation was analyzed using cell apoptosis assay. Results Increased DLEU1 expression and decreased miR-146b-5p expression were observed in POF. DLEU1 directly interacted with MiR-146b-5p and was expressed in both nuclear and cytoplasm samples of KGN cells. In KGN cells, DLEU1 and miR-146b-5p failed to regulate the expression of each other. However, DLEU1 promoted cell apoptosis and reduced the inhibitory effects of miR-146b-5p on cell apoptosis. Conclusions DLEU1 is overexpressed in POF and sponges miR-146b-5p to increase KGN cell apoptosis.
format	article
author	Caihong Zheng Shiwei Liu Zhihong Qin Xiaoqian Zhang Yubao Song
author_facet	Caihong Zheng Shiwei Liu Zhihong Qin Xiaoqian Zhang Yubao Song
author_sort	Caihong Zheng
title	LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis
title_short	LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis
title_full	LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis
title_fullStr	LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis
title_full_unstemmed	LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis
title_sort	lncrna dleu1 is overexpressed in premature ovarian failure and sponges mir-146b-5p to increase granulosa cell apoptosis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/966b7ec8588b44d6966f69500f158b75
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LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis

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