Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes

Although most birds show karyotypes with diploid number (2n) around 80, with few macrochromosomes and many microchromosomes pairs, some groups, such as the Accipitriformes, are characterized by a large karyotypic reorganization, which resulted in complements with low diploid numbers, and a smaller n...

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Autores principales: Carlos A. Carvalho, Ivanete O. Furo, Patricia C. M. O’Brien, Jorge Pereira, Rebeca E. O’Connor, Darren Griffin, Malcolm Ferguson-Smith, Edivaldo Herculano Corrêa de Oliveira
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Publicado: Public Library of Science (PLoS) 2021
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spelling oai:doaj.org-article:966b9f7fdcac48a799364f2de92b379d2021-11-25T06:19:44ZComparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes1932-6203https://doaj.org/article/966b9f7fdcac48a799364f2de92b379d2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601422/?tool=EBIhttps://doaj.org/toc/1932-6203Although most birds show karyotypes with diploid number (2n) around 80, with few macrochromosomes and many microchromosomes pairs, some groups, such as the Accipitriformes, are characterized by a large karyotypic reorganization, which resulted in complements with low diploid numbers, and a smaller number of microchromosomal pairs when compared to other birds. Among Accipitriformes, the Accipitridae family is the most diverse and includes, among other subfamilies, the subfamily Aquilinae, composed of medium to large sized species. The Black-Hawk-Eagle (Spizaetus tyrannus-STY), found in South America, is a member of this subfamily. Available chromosome data for this species includes only conventional staining. Hence, in order to provide additional information on karyotype evolution process within this group, we performed comparative chromosome painting between S. tyrannus and Gallus gallus (GGA). Our results revealed that at least 29 fission-fusion events occurred in the STY karyotype, based on homology with GGA. Fissions occurred mainly in syntenic groups homologous to GGA1-GGA5. On the other hand, the majority of the microchromosomes were found fused to other chromosomal elements in STY, indicating these rearrangements played an important role in the reduction of the 2n to 68. Comparison with hybridization pattern of the Japanese-Mountain-Eagle (Nisaetus nipalensis orientalis), the only Aquilinae analyzed by comparative chromosome painting previously, did not reveal any synapomorphy that could represent a chromosome signature to this subfamily. Therefore, conclusions about karyotype evolution in Aquilinae require additional painting studies.Carlos A. CarvalhoIvanete O. FuroPatricia C. M. O’BrienJorge PereiraRebeca E. O’ConnorDarren GriffinMalcolm Ferguson-SmithEdivaldo Herculano Corrêa de OliveiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Carlos A. Carvalho
Ivanete O. Furo
Patricia C. M. O’Brien
Jorge Pereira
Rebeca E. O’Connor
Darren Griffin
Malcolm Ferguson-Smith
Edivaldo Herculano Corrêa de Oliveira
Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes
description Although most birds show karyotypes with diploid number (2n) around 80, with few macrochromosomes and many microchromosomes pairs, some groups, such as the Accipitriformes, are characterized by a large karyotypic reorganization, which resulted in complements with low diploid numbers, and a smaller number of microchromosomal pairs when compared to other birds. Among Accipitriformes, the Accipitridae family is the most diverse and includes, among other subfamilies, the subfamily Aquilinae, composed of medium to large sized species. The Black-Hawk-Eagle (Spizaetus tyrannus-STY), found in South America, is a member of this subfamily. Available chromosome data for this species includes only conventional staining. Hence, in order to provide additional information on karyotype evolution process within this group, we performed comparative chromosome painting between S. tyrannus and Gallus gallus (GGA). Our results revealed that at least 29 fission-fusion events occurred in the STY karyotype, based on homology with GGA. Fissions occurred mainly in syntenic groups homologous to GGA1-GGA5. On the other hand, the majority of the microchromosomes were found fused to other chromosomal elements in STY, indicating these rearrangements played an important role in the reduction of the 2n to 68. Comparison with hybridization pattern of the Japanese-Mountain-Eagle (Nisaetus nipalensis orientalis), the only Aquilinae analyzed by comparative chromosome painting previously, did not reveal any synapomorphy that could represent a chromosome signature to this subfamily. Therefore, conclusions about karyotype evolution in Aquilinae require additional painting studies.
format article
author Carlos A. Carvalho
Ivanete O. Furo
Patricia C. M. O’Brien
Jorge Pereira
Rebeca E. O’Connor
Darren Griffin
Malcolm Ferguson-Smith
Edivaldo Herculano Corrêa de Oliveira
author_facet Carlos A. Carvalho
Ivanete O. Furo
Patricia C. M. O’Brien
Jorge Pereira
Rebeca E. O’Connor
Darren Griffin
Malcolm Ferguson-Smith
Edivaldo Herculano Corrêa de Oliveira
author_sort Carlos A. Carvalho
title Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes
title_short Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes
title_full Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes
title_fullStr Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes
title_full_unstemmed Comparative chromosome painting in Spizaetus tyrannus and Gallus gallus with the use of macro- and microchromosome probes
title_sort comparative chromosome painting in spizaetus tyrannus and gallus gallus with the use of macro- and microchromosome probes
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/966b9f7fdcac48a799364f2de92b379d
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