Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.

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Autores principales: Ashot Papikian, Wanlu Liu, Javier Gallego-Bartolomé, Steven E. Jacobsen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/966d08acb09448ee9fe2347f8253f363
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spelling oai:doaj.org-article:966d08acb09448ee9fe2347f8253f3632021-12-02T17:02:03ZSite-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems10.1038/s41467-019-08736-72041-1723https://doaj.org/article/966d08acb09448ee9fe2347f8253f3632019-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08736-7https://doaj.org/toc/2041-1723Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.Ashot PapikianWanlu LiuJavier Gallego-BartoloméSteven E. JacobsenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ashot Papikian
Wanlu Liu
Javier Gallego-Bartolomé
Steven E. Jacobsen
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
description Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.
format article
author Ashot Papikian
Wanlu Liu
Javier Gallego-Bartolomé
Steven E. Jacobsen
author_facet Ashot Papikian
Wanlu Liu
Javier Gallego-Bartolomé
Steven E. Jacobsen
author_sort Ashot Papikian
title Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_short Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_full Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_fullStr Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_full_unstemmed Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_sort site-specific manipulation of arabidopsis loci using crispr-cas9 suntag systems
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/966d08acb09448ee9fe2347f8253f363
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AT wanluliu sitespecificmanipulationofarabidopsislociusingcrisprcas9suntagsystems
AT javiergallegobartolome sitespecificmanipulationofarabidopsislociusingcrisprcas9suntagsystems
AT stevenejacobsen sitespecificmanipulationofarabidopsislociusingcrisprcas9suntagsystems
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