Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study
Abstract Big cats (Genus: Panthera) are among the most threatened mammal groups of the world, owing to hunting, habitat loss, and illegal transnational trade. Conservation genetic studies and effective curbs on poaching are important for the conservation of these charismatic apex predators. A limite...
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2021
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oai:doaj.org-article:967c6e9348704f2cb339b139493de7162021-12-02T15:23:07ZWhole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study10.1038/s41598-021-92781-02045-2322https://doaj.org/article/967c6e9348704f2cb339b139493de7162021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92781-0https://doaj.org/toc/2045-2322Abstract Big cats (Genus: Panthera) are among the most threatened mammal groups of the world, owing to hunting, habitat loss, and illegal transnational trade. Conservation genetic studies and effective curbs on poaching are important for the conservation of these charismatic apex predators. A limited number of microsatellite markers exists for Panthera species and researchers often cross-amplify domestic cat microsatellites to study these species. We conducted data mining of seven Panthera genome sequences to discover microsatellites for conservation genetic studies of four threatened big cat species. A total of 32 polymorphic microsatellite loci were identified in silico and tested with 152 big cats, and were found polymorphic in most of the tested species. We propose a set of 12 novel microsatellite markers for use in conservation genetics and wildlife forensic investigations of big cat species. Cumulatively, these markers have a high discriminatory power of one in a million for unrelated individuals and one in a thousand for siblings. Similar PCR conditions of these markers increase the prospects of achieving efficient multiplex PCR assays. This study is a pioneering attempt to synthesise genome wide microsatellite markers for big cats.Jee Yun HyunPuneet PandeyKyung Seok KimAlvin ChonDaecheol JeongJong BhakMihyeon YuHye Kyung SongRandeep SinghMi-Sook MinSurendra Prakash GoyalDamdingiin BayarkhagvaTaisia MarchenkovaAnna VitkalovaHang LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
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Medicine R Science Q Jee Yun Hyun Puneet Pandey Kyung Seok Kim Alvin Chon Daecheol Jeong Jong Bhak Mihyeon Yu Hye Kyung Song Randeep Singh Mi-Sook Min Surendra Prakash Goyal Damdingiin Bayarkhagva Taisia Marchenkova Anna Vitkalova Hang Lee Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study |
| description |
Abstract Big cats (Genus: Panthera) are among the most threatened mammal groups of the world, owing to hunting, habitat loss, and illegal transnational trade. Conservation genetic studies and effective curbs on poaching are important for the conservation of these charismatic apex predators. A limited number of microsatellite markers exists for Panthera species and researchers often cross-amplify domestic cat microsatellites to study these species. We conducted data mining of seven Panthera genome sequences to discover microsatellites for conservation genetic studies of four threatened big cat species. A total of 32 polymorphic microsatellite loci were identified in silico and tested with 152 big cats, and were found polymorphic in most of the tested species. We propose a set of 12 novel microsatellite markers for use in conservation genetics and wildlife forensic investigations of big cat species. Cumulatively, these markers have a high discriminatory power of one in a million for unrelated individuals and one in a thousand for siblings. Similar PCR conditions of these markers increase the prospects of achieving efficient multiplex PCR assays. This study is a pioneering attempt to synthesise genome wide microsatellite markers for big cats. |
| format |
article |
| author |
Jee Yun Hyun Puneet Pandey Kyung Seok Kim Alvin Chon Daecheol Jeong Jong Bhak Mihyeon Yu Hye Kyung Song Randeep Singh Mi-Sook Min Surendra Prakash Goyal Damdingiin Bayarkhagva Taisia Marchenkova Anna Vitkalova Hang Lee |
| author_facet |
Jee Yun Hyun Puneet Pandey Kyung Seok Kim Alvin Chon Daecheol Jeong Jong Bhak Mihyeon Yu Hye Kyung Song Randeep Singh Mi-Sook Min Surendra Prakash Goyal Damdingiin Bayarkhagva Taisia Marchenkova Anna Vitkalova Hang Lee |
| author_sort |
Jee Yun Hyun |
| title |
Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study |
| title_short |
Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study |
| title_full |
Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study |
| title_fullStr |
Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study |
| title_full_unstemmed |
Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study |
| title_sort |
whole genome survey of big cats (genus: panthera) identifies novel microsatellites of utility in conservation genetic study |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/967c6e9348704f2cb339b139493de716 |
| work_keys_str_mv |
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