Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells

Background and purpose: One of the most effective methods for the development of dendritic cell (DC)-based cancer immunotherapy is ex vivo pulsing of DCs with tumor cell lysates (TCLs). However, antitumor immune responses of DCs are significantly influenced by how TCLs were prepared. Here, we compar...

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Autores principales:	Mohammad Roufarshbaf, Nafiseh Esmaeil, Vajihe Akbari
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	antigen loading; colorectal cancer; dendritic cell; hypochlorous acid tumor cell lysate. Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/967ef07eb1bb4a569d4a036d15ba47c1
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spelling	oai:doaj.org-article:967ef07eb1bb4a569d4a036d15ba47c12021-11-19T12:17:03ZComparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells1735-53621735-941410.4103/1735-5362.329925https://doaj.org/article/967ef07eb1bb4a569d4a036d15ba47c12022-01-01T00:00:00Zhttp://www.rpsjournal.net/article.asp?issn=1735-5362;year=2022;volume=17;issue=1;spage=43;epage=52;aulast=Roufarshbafhttps://doaj.org/toc/1735-5362https://doaj.org/toc/1735-9414Background and purpose: One of the most effective methods for the development of dendritic cell (DC)-based cancer immunotherapy is ex vivo pulsing of DCs with tumor cell lysates (TCLs). However, antitumor immune responses of DCs are significantly influenced by how TCLs were prepared. Here, we compared four strategies of TCL preparation derived from colon cancer cells, HT-29, for ex vivo maturation of DCs. Experimental approach: Peripheral blood monocytes were isolated from healthy volunteers and incubated with granulocyte macrophage colony-stimulating factor and interleukin (IL)-4 to differentiate into DCs in 10 days. Morphological properties, phenotype characteristics (i.e. CD83 and CD86), and cytokine production (i.e. IL-10 and interferon gamma) of DCs loaded with four different TCLs (i.e. freeze-thaw, hypochlorous acid (HOCl), hyperthermia, and UV irradiation) were evaluated. Findings/Results: HOCl preparations led to the generation of DCs with higher surface expression of maturation biomarkers (particularly CD83), while UV preparations resulted in DCs with lower levels of surface biomarkers compared to freeze-thawed preparations. The supernatant of DCs pulsed with HOCl preparation showed significantly higher levels of interferon gamma and lower levels of IL-10 compared with the other groups. Conclusion and implications: Our results suggest that pulsing DCs with HOCl preparation may be superior to other TCLs preparation strategies, possibly due to induction of rapid necrotic cell death.Mohammad RoufarshbafNafiseh EsmaeilVajihe AkbariWolters Kluwer Medknow Publicationsarticleantigen loading; colorectal cancer; dendritic cell; hypochlorous acidtumor cell lysate.Pharmacy and materia medicaRS1-441ENResearch in Pharmaceutical Sciences, Vol 17, Iss 1, Pp 43-52 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	antigen loading; colorectal cancer; dendritic cell; hypochlorous acid tumor cell lysate. Pharmacy and materia medica RS1-441
spellingShingle	antigen loading; colorectal cancer; dendritic cell; hypochlorous acid tumor cell lysate. Pharmacy and materia medica RS1-441 Mohammad Roufarshbaf Nafiseh Esmaeil Vajihe Akbari Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
description	Background and purpose: One of the most effective methods for the development of dendritic cell (DC)-based cancer immunotherapy is ex vivo pulsing of DCs with tumor cell lysates (TCLs). However, antitumor immune responses of DCs are significantly influenced by how TCLs were prepared. Here, we compared four strategies of TCL preparation derived from colon cancer cells, HT-29, for ex vivo maturation of DCs. Experimental approach: Peripheral blood monocytes were isolated from healthy volunteers and incubated with granulocyte macrophage colony-stimulating factor and interleukin (IL)-4 to differentiate into DCs in 10 days. Morphological properties, phenotype characteristics (i.e. CD83 and CD86), and cytokine production (i.e. IL-10 and interferon gamma) of DCs loaded with four different TCLs (i.e. freeze-thaw, hypochlorous acid (HOCl), hyperthermia, and UV irradiation) were evaluated. Findings/Results: HOCl preparations led to the generation of DCs with higher surface expression of maturation biomarkers (particularly CD83), while UV preparations resulted in DCs with lower levels of surface biomarkers compared to freeze-thawed preparations. The supernatant of DCs pulsed with HOCl preparation showed significantly higher levels of interferon gamma and lower levels of IL-10 compared with the other groups. Conclusion and implications: Our results suggest that pulsing DCs with HOCl preparation may be superior to other TCLs preparation strategies, possibly due to induction of rapid necrotic cell death.
format	article
author	Mohammad Roufarshbaf Nafiseh Esmaeil Vajihe Akbari
author_facet	Mohammad Roufarshbaf Nafiseh Esmaeil Vajihe Akbari
author_sort	Mohammad Roufarshbaf
title	Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
title_short	Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
title_full	Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
title_fullStr	Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
title_full_unstemmed	Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
title_sort	comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/967ef07eb1bb4a569d4a036d15ba47c1
work_keys_str_mv	AT mohammadroufarshbaf comparisonoffourmethodsofcoloncancercelllysatespreparationforexvivomaturationofdendriticcells AT nafisehesmaeil comparisonoffourmethodsofcoloncancercelllysatespreparationforexvivomaturationofdendriticcells AT vajiheakbari comparisonoffourmethodsofcoloncancercelllysatespreparationforexvivomaturationofdendriticcells
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Comparison of four methods of colon cancer cell lysates preparation for ex vivo maturation of dendritic cells

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