Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation

G&eacute;rrard Eddy Jai Poinern1, Xuan Thi Le1, Songhua Shan2, Trevor Ellis3, Stan Fenwick3, John Edwards3, Derek Fawcett11Murdoch Applied Nanotechnology Research Group, Murdoch University, Murdoch, WA, Australia; 2Australian Animal Health Laboratories, CSIRO, VIC, Australia; 3Veterinary Sch...

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Autores principales:	Poinern GEJ, Thi Le X, Shan S, Ellis T, Fenwick S, Edwards J, Fawcett D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9680efa90d3d487ba029daeadfcd7007
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spelling	oai:doaj.org-article:9680efa90d3d487ba029daeadfcd70072021-12-02T00:18:47ZUltrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation1176-91141178-2013https://doaj.org/article/9680efa90d3d487ba029daeadfcd70072011-09-01T00:00:00Zhttp://www.dovepress.com/ultrasonic-synthetic-technique-to-manufacture-a-phema-nanopolymeric-ba-a8374https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013G&eacute;rrard Eddy Jai Poinern1, Xuan Thi Le1, Songhua Shan2, Trevor Ellis3, Stan Fenwick3, John Edwards3, Derek Fawcett11Murdoch Applied Nanotechnology Research Group, Murdoch University, Murdoch, WA, Australia; 2Australian Animal Health Laboratories, CSIRO, VIC, Australia; 3Veterinary School, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, AustraliaAbstract: This preliminary study investigated the use of poly (2-hydroxyethyl methacrylate) (pHEMA) nanoparticles for the delivery of the deoxyribonucleic acid (DNA) vaccine pCAG-HAk, which expresses the full length hemagglutinin (HA) gene of the avian influenza A/Eurasian coot/Western Australian/2727/1979 (H6N2) virus with a Kozak sequence which is in the form of a pCAGGS vector. The loaded and unloaded nanoparticles were characterized using field-emission scanning electron microscopy. Further characterizations of the nanoparticles were made using atomic force microscopy and dynamic light scattering, which was used to investigate particle size distributions. This preliminary study suggests that using 100 &micro;g of pHEMA nanoparticles as a nanocarrier/adjuvant produced a reduction in virus shedding and improved the immune response to the DNA vaccine pCAG-HAk.Keywords: nanoparticles, ultrasound, deoxyribonucleic acid, plasmid, bird flu, pandemicPoinern GEJThi Le XShan SEllis TFenwick SEdwards JFawcett DDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2167-2174 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Poinern GEJ Thi Le X Shan S Ellis T Fenwick S Edwards J Fawcett D Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
description	G&eacute;rrard Eddy Jai Poinern1, Xuan Thi Le1, Songhua Shan2, Trevor Ellis3, Stan Fenwick3, John Edwards3, Derek Fawcett11Murdoch Applied Nanotechnology Research Group, Murdoch University, Murdoch, WA, Australia; 2Australian Animal Health Laboratories, CSIRO, VIC, Australia; 3Veterinary School, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, AustraliaAbstract: This preliminary study investigated the use of poly (2-hydroxyethyl methacrylate) (pHEMA) nanoparticles for the delivery of the deoxyribonucleic acid (DNA) vaccine pCAG-HAk, which expresses the full length hemagglutinin (HA) gene of the avian influenza A/Eurasian coot/Western Australian/2727/1979 (H6N2) virus with a Kozak sequence which is in the form of a pCAGGS vector. The loaded and unloaded nanoparticles were characterized using field-emission scanning electron microscopy. Further characterizations of the nanoparticles were made using atomic force microscopy and dynamic light scattering, which was used to investigate particle size distributions. This preliminary study suggests that using 100 &micro;g of pHEMA nanoparticles as a nanocarrier/adjuvant produced a reduction in virus shedding and improved the immune response to the DNA vaccine pCAG-HAk.Keywords: nanoparticles, ultrasound, deoxyribonucleic acid, plasmid, bird flu, pandemic
format	article
author	Poinern GEJ Thi Le X Shan S Ellis T Fenwick S Edwards J Fawcett D
author_facet	Poinern GEJ Thi Le X Shan S Ellis T Fenwick S Edwards J Fawcett D
author_sort	Poinern GEJ
title	Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
title_short	Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
title_full	Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
title_fullStr	Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
title_full_unstemmed	Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation
title_sort	ultrasonic synthetic technique to manufacture a phema nanopolymeric-based vaccine against the h6n2 avian influenza virus: a preliminary investigation
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/9680efa90d3d487ba029daeadfcd7007
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Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation

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