Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential

Obese sarcopenia is associated with palmitic acid (PA), an abundant circulating saturated fatty acid. This study examined a non-cytotoxic concentration of PA to provide mechanistic insights into PA-impaired skeletal myogenesis and potential medicinal and dietary interventions through edible brown se...

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Autores principales:	Thilina U. Jayawardena, D.P. Nagahawatta, Yu-An Lu, Hye-Won Yang, Jun-Geon Je, Seo-Young Kim, You-Jin Jeon
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Palmitic acid Skeletal myogenesis Myogenic markers Adipogenic markers Growth regulators Ishige okamurae Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/96850050689a4790b35615036d0e73fb
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spelling	oai:doaj.org-article:96850050689a4790b35615036d0e73fb2021-11-14T04:31:22ZIshige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential1756-464610.1016/j.jff.2021.104832https://doaj.org/article/96850050689a4790b35615036d0e73fb2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1756464621004813https://doaj.org/toc/1756-4646Obese sarcopenia is associated with palmitic acid (PA), an abundant circulating saturated fatty acid. This study examined a non-cytotoxic concentration of PA to provide mechanistic insights into PA-impaired skeletal myogenesis and potential medicinal and dietary interventions through edible brown seaweed, Ishige okamurae (IO). C2C12 cells were examined for myogenic markers, adipogenic factors, and regenerative capacity through growth regulators against PA interference to assess IO and purified diphloroethohydoxycarmalol (DPHC) as potential treatments. Both IO and DPHC improved myogenic marker (myogenin, MyoD, and MyHC) levels. PA down-regulated myogenic markers while improving adipogenic factors (PPARγ, c/EBPα, A-FABP), DPHC significantly arbitrated the negative effects. DPHC treatment also improved phosphorylation of the growth regulatory PI3K/Akt/mTOR axis over the adverse effects of PA. The results of this study suggested regulatory mechanisms through which the bioactive components IO and DPHC based on the sustainable utilization of I. okamurae inhibited the PA-induced impairment of skeletal myogenesis.Thilina U. JayawardenaD.P. NagahawattaYu-An LuHye-Won YangJun-Geon JeSeo-Young KimYou-Jin JeonElsevierarticlePalmitic acidSkeletal myogenesisMyogenic markersAdipogenic markersGrowth regulatorsIshige okamuraeNutrition. Foods and food supplyTX341-641ENJournal of Functional Foods, Vol 87, Iss , Pp 104832- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Palmitic acid Skeletal myogenesis Myogenic markers Adipogenic markers Growth regulators Ishige okamurae Nutrition. Foods and food supply TX341-641
spellingShingle	Palmitic acid Skeletal myogenesis Myogenic markers Adipogenic markers Growth regulators Ishige okamurae Nutrition. Foods and food supply TX341-641 Thilina U. Jayawardena D.P. Nagahawatta Yu-An Lu Hye-Won Yang Jun-Geon Je Seo-Young Kim You-Jin Jeon Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
description	Obese sarcopenia is associated with palmitic acid (PA), an abundant circulating saturated fatty acid. This study examined a non-cytotoxic concentration of PA to provide mechanistic insights into PA-impaired skeletal myogenesis and potential medicinal and dietary interventions through edible brown seaweed, Ishige okamurae (IO). C2C12 cells were examined for myogenic markers, adipogenic factors, and regenerative capacity through growth regulators against PA interference to assess IO and purified diphloroethohydoxycarmalol (DPHC) as potential treatments. Both IO and DPHC improved myogenic marker (myogenin, MyoD, and MyHC) levels. PA down-regulated myogenic markers while improving adipogenic factors (PPARγ, c/EBPα, A-FABP), DPHC significantly arbitrated the negative effects. DPHC treatment also improved phosphorylation of the growth regulatory PI3K/Akt/mTOR axis over the adverse effects of PA. The results of this study suggested regulatory mechanisms through which the bioactive components IO and DPHC based on the sustainable utilization of I. okamurae inhibited the PA-induced impairment of skeletal myogenesis.
format	article
author	Thilina U. Jayawardena D.P. Nagahawatta Yu-An Lu Hye-Won Yang Jun-Geon Je Seo-Young Kim You-Jin Jeon
author_facet	Thilina U. Jayawardena D.P. Nagahawatta Yu-An Lu Hye-Won Yang Jun-Geon Je Seo-Young Kim You-Jin Jeon
author_sort	Thilina U. Jayawardena
title	Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
title_short	Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
title_full	Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
title_fullStr	Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
title_full_unstemmed	Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
title_sort	ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/96850050689a4790b35615036d0e73fb
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Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential

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