Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis

Jane Yoo,1 Anne M Manicone,2 John K McGuire,3 Ying Wang,2 William C Parks2 1Center for Lung Biology, Department of Medicine, Division of Dermatology, 2Division of Pulmonary and Critical Care Medicine, 3Department of Pediatrics, Division Critical Care Medicine, University of Washington, Seattle, WA,...

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Autores principales:	Yoo J, Manicone AM, McGuire JK, Wang Y, Parks WC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9685841f6eb34600af78dc452526f08f
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spelling	oai:doaj.org-article:9685841f6eb34600af78dc452526f08f2021-12-02T00:24:39ZSystemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis1178-7031https://doaj.org/article/9685841f6eb34600af78dc452526f08f2014-02-01T00:00:00Zhttp://www.dovepress.com/systemic-sensitization-with-the-protein-allergen-ovalbumin-augments-lo-a15900https://doaj.org/toc/1178-7031 Jane Yoo,1 Anne M Manicone,2 John K McGuire,3 Ying Wang,2 William C Parks2 1Center for Lung Biology, Department of Medicine, Division of Dermatology, 2Division of Pulmonary and Critical Care Medicine, 3Department of Pediatrics, Division Critical Care Medicine, University of Washington, Seattle, WA, USA Abstract: Mouse models of atopic dermatitis based on epicutaneous sensitization have shed light on the role of epicutaneous allergen entry in the development of respiratory and gastrointestinal allergy. However, the contribution of non-cutaneous modes of sensitization to skin diseases has not been evaluated. We assessed if systemic ovalbumin administration, in conjunction with local sensitization, could prime for a robust inflammatory response. Furthermore, we attempted to elucidate important aspects of disease pathogenesis previously unaddressed in mouse models. Mice that underwent intraperitoneal ovalbumin sensitization prior to epicutaneous challenge demonstrated an acute (Th2-polarized) atopic dermatitis-like phenotype upon local challenge. The inflammatory response was strikingly more robust than in mice that underwent epicutaneous sensitization alone. The lesional infiltrate contained a dendritic cell population that corresponded phenotypically with inflammatory dendritic epidermal cells of significance in human disease. Finally, in accordance with observations in human atopic dermatitis, there was an increase in cluster of differentiation (CD) 103 (αE subunit)-expressing CD4+ T lymphocytes. However, the absence of CD103 on approximately 50% of infiltrating cells argues against a primary role for the αEβ7 integrin in tissue homing. In conclusion, we present a mouse model of atopic dermatitis that reveals novel insights into the pathogenesis of this complex disease. Keywords: atopic dermatitis, mouse model, ovalbumin, sensitization, Th2, dendritic cellsYoo JManicone AMMcGuire JKWang YParks WCDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2014, Iss default, Pp 29-38 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Yoo J Manicone AM McGuire JK Wang Y Parks WC Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
description	Jane Yoo,1 Anne M Manicone,2 John K McGuire,3 Ying Wang,2 William C Parks2 1Center for Lung Biology, Department of Medicine, Division of Dermatology, 2Division of Pulmonary and Critical Care Medicine, 3Department of Pediatrics, Division Critical Care Medicine, University of Washington, Seattle, WA, USA Abstract: Mouse models of atopic dermatitis based on epicutaneous sensitization have shed light on the role of epicutaneous allergen entry in the development of respiratory and gastrointestinal allergy. However, the contribution of non-cutaneous modes of sensitization to skin diseases has not been evaluated. We assessed if systemic ovalbumin administration, in conjunction with local sensitization, could prime for a robust inflammatory response. Furthermore, we attempted to elucidate important aspects of disease pathogenesis previously unaddressed in mouse models. Mice that underwent intraperitoneal ovalbumin sensitization prior to epicutaneous challenge demonstrated an acute (Th2-polarized) atopic dermatitis-like phenotype upon local challenge. The inflammatory response was strikingly more robust than in mice that underwent epicutaneous sensitization alone. The lesional infiltrate contained a dendritic cell population that corresponded phenotypically with inflammatory dendritic epidermal cells of significance in human disease. Finally, in accordance with observations in human atopic dermatitis, there was an increase in cluster of differentiation (CD) 103 (αE subunit)-expressing CD4+ T lymphocytes. However, the absence of CD103 on approximately 50% of infiltrating cells argues against a primary role for the αEβ7 integrin in tissue homing. In conclusion, we present a mouse model of atopic dermatitis that reveals novel insights into the pathogenesis of this complex disease. Keywords: atopic dermatitis, mouse model, ovalbumin, sensitization, Th2, dendritic cells
format	article
author	Yoo J Manicone AM McGuire JK Wang Y Parks WC
author_facet	Yoo J Manicone AM McGuire JK Wang Y Parks WC
author_sort	Yoo J
title	Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
title_short	Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
title_full	Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
title_fullStr	Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
title_full_unstemmed	Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
title_sort	systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/9685841f6eb34600af78dc452526f08f
work_keys_str_mv	AT yooj systemicsensitizationwiththeproteinallergenovalbuminaugmentslocalsensitizationinatopicdermatitis AT maniconeam systemicsensitizationwiththeproteinallergenovalbuminaugmentslocalsensitizationinatopicdermatitis AT mcguirejk systemicsensitizationwiththeproteinallergenovalbuminaugmentslocalsensitizationinatopicdermatitis AT wangy systemicsensitizationwiththeproteinallergenovalbuminaugmentslocalsensitizationinatopicdermatitis AT parkswc systemicsensitizationwiththeproteinallergenovalbuminaugmentslocalsensitizationinatopicdermatitis
_version_	1718403753235709952

Systemic sensitization with the protein allergen ovalbumin augments local sensitization in atopic dermatitis

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