Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken

Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897. Myostati...

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Autores principales:	Linda Suhartati, ISYANA KHAERUNNISA, ASEP GUNAWAN, RUKMIASIH RUKMIASIH, SRI DARWATI, CECE SUMANTRI, RIZQAN RIZQAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	kampung chicken, meat-type chicken, myostatin gene, pcr-rflp, slaughtered weight Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/96869dabd2e345eb8b17fa0c21ab24ad
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spelling	oai:doaj.org-article:96869dabd2e345eb8b17fa0c21ab24ad2021-11-22T12:17:57ZShort Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken1412-033X2085-472210.13057/biodiv/d210860https://doaj.org/article/96869dabd2e345eb8b17fa0c21ab24ad2020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6159https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897. Myostatin is widely known as a negative regulator of skeletal muscle development by controlling hypertrophy and hyperplasia. Silent mutation in exon 1 (c.234G>A) was then thought to be associated with chicken growth performances. This study was designed to analyze polymorphisms of c.234G>A mutation of the myostatin gene and its effect on slaughtered weight in Indonesian Kampung and meat-type chicken. The SatI restriction enzyme was used for genotype determination through the PCR-RFLP technique. The effect of genotype on the slaughtered weight was analyzed using the General Linear Model (GLM) procedure. Genotyping was performed on 138 chicken from Kampung (n=67) and meat type (cobb Broiler) (n=71) chicken. The amplification product contained 226 bp nucleotides. This myostatin gene was polymorphic in both chicken population, produced 2 alleles (G and A), and 3 genotypes (GG, GA, and AA). The A allele had a higher frequency than the G allele in all populations. In this study, genotype of AA and GA had higher live weight, carcass weight, breast weight, thigh weight, drumsticks weight, wings weight, breast muscle weight, thighs muscle weight, drumsticks muscle weight than GG genotype of 12 weeks-old Kampung chicken. The results suggested that the c.234G>A mutation in exon 1 could be potentially recommended as a genetic marker for chicken slaughtered weight traits.Linda SuhartatiISYANA KHAERUNNISAASEP GUNAWANRUKMIASIH RUKMIASIHSRI DARWATICECE SUMANTRIRIZQAN RIZQANMBI & UNS Soloarticlekampung chicken, meat-type chicken, myostatin gene, pcr-rflp, slaughtered weightBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	kampung chicken, meat-type chicken, myostatin gene, pcr-rflp, slaughtered weight Biology (General) QH301-705.5
spellingShingle	kampung chicken, meat-type chicken, myostatin gene, pcr-rflp, slaughtered weight Biology (General) QH301-705.5 Linda Suhartati ISYANA KHAERUNNISA ASEP GUNAWAN RUKMIASIH RUKMIASIH SRI DARWATI CECE SUMANTRI RIZQAN RIZQAN Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken
description	Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897. Myostatin is widely known as a negative regulator of skeletal muscle development by controlling hypertrophy and hyperplasia. Silent mutation in exon 1 (c.234G>A) was then thought to be associated with chicken growth performances. This study was designed to analyze polymorphisms of c.234G>A mutation of the myostatin gene and its effect on slaughtered weight in Indonesian Kampung and meat-type chicken. The SatI restriction enzyme was used for genotype determination through the PCR-RFLP technique. The effect of genotype on the slaughtered weight was analyzed using the General Linear Model (GLM) procedure. Genotyping was performed on 138 chicken from Kampung (n=67) and meat type (cobb Broiler) (n=71) chicken. The amplification product contained 226 bp nucleotides. This myostatin gene was polymorphic in both chicken population, produced 2 alleles (G and A), and 3 genotypes (GG, GA, and AA). The A allele had a higher frequency than the G allele in all populations. In this study, genotype of AA and GA had higher live weight, carcass weight, breast weight, thigh weight, drumsticks weight, wings weight, breast muscle weight, thighs muscle weight, drumsticks muscle weight than GG genotype of 12 weeks-old Kampung chicken. The results suggested that the c.234G>A mutation in exon 1 could be potentially recommended as a genetic marker for chicken slaughtered weight traits.
format	article
author	Linda Suhartati ISYANA KHAERUNNISA ASEP GUNAWAN RUKMIASIH RUKMIASIH SRI DARWATI CECE SUMANTRI RIZQAN RIZQAN
author_facet	Linda Suhartati ISYANA KHAERUNNISA ASEP GUNAWAN RUKMIASIH RUKMIASIH SRI DARWATI CECE SUMANTRI RIZQAN RIZQAN
author_sort	Linda Suhartati
title	Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken
title_short	Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken
title_full	Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken
title_fullStr	Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken
title_full_unstemmed	Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken
title_sort	short communication: identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in indonesian kampung and broiler chicken
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/96869dabd2e345eb8b17fa0c21ab24ad
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Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken

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