Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals

Abstract The protein content of urinary extracellular vesicles (EVs) is considered to be an attractive non-invasive biomarker source. However, little is known about the consistency and variability of urinary EV proteins within and between individuals over a longer time-period. Here, we evaluated the...

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Autores principales:	Leyla A. Erozenci, Sander R. Piersma, Thang V. Pham, Irene V. Bijnsdorp, Connie R. Jimenez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9686ce98b6f64f4faf7617ce8e795119
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spelling	oai:doaj.org-article:9686ce98b6f64f4faf7617ce8e7951192021-12-02T16:35:32ZLongitudinal stability of urinary extracellular vesicle protein patterns within and between individuals10.1038/s41598-021-95082-82045-2322https://doaj.org/article/9686ce98b6f64f4faf7617ce8e7951192021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95082-8https://doaj.org/toc/2045-2322Abstract The protein content of urinary extracellular vesicles (EVs) is considered to be an attractive non-invasive biomarker source. However, little is known about the consistency and variability of urinary EV proteins within and between individuals over a longer time-period. Here, we evaluated the stability of the urinary EV proteomes of 8 healthy individuals at 9 timepoints over 6 months using data-independent-acquisition mass spectrometry. The 1802 identified proteins had a high correlation amongst all samples, with 40% of the proteome detected in every sample and 90% detected in more than 1 individual at all timepoints. Unsupervised analysis of top 10% most variable proteins yielded person-specific profiles. The core EV-protein-interaction network of 516 proteins detected in all measured samples revealed sub-clusters involved in the biological processes of G-protein signaling, cytoskeletal transport, cellular energy metabolism and immunity. Furthermore, gender-specific expression patterns were detected in the urinary EV proteome. Our findings indicate that the urinary EV proteome is stable in longitudinal samples of healthy subjects over a prolonged time-period, further underscoring its potential for reliable non-invasive diagnostic/prognostic biomarkers.Leyla A. ErozenciSander R. PiersmaThang V. PhamIrene V. BijnsdorpConnie R. JimenezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leyla A. Erozenci Sander R. Piersma Thang V. Pham Irene V. Bijnsdorp Connie R. Jimenez Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
description	Abstract The protein content of urinary extracellular vesicles (EVs) is considered to be an attractive non-invasive biomarker source. However, little is known about the consistency and variability of urinary EV proteins within and between individuals over a longer time-period. Here, we evaluated the stability of the urinary EV proteomes of 8 healthy individuals at 9 timepoints over 6 months using data-independent-acquisition mass spectrometry. The 1802 identified proteins had a high correlation amongst all samples, with 40% of the proteome detected in every sample and 90% detected in more than 1 individual at all timepoints. Unsupervised analysis of top 10% most variable proteins yielded person-specific profiles. The core EV-protein-interaction network of 516 proteins detected in all measured samples revealed sub-clusters involved in the biological processes of G-protein signaling, cytoskeletal transport, cellular energy metabolism and immunity. Furthermore, gender-specific expression patterns were detected in the urinary EV proteome. Our findings indicate that the urinary EV proteome is stable in longitudinal samples of healthy subjects over a prolonged time-period, further underscoring its potential for reliable non-invasive diagnostic/prognostic biomarkers.
format	article
author	Leyla A. Erozenci Sander R. Piersma Thang V. Pham Irene V. Bijnsdorp Connie R. Jimenez
author_facet	Leyla A. Erozenci Sander R. Piersma Thang V. Pham Irene V. Bijnsdorp Connie R. Jimenez
author_sort	Leyla A. Erozenci
title	Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
title_short	Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
title_full	Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
title_fullStr	Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
title_full_unstemmed	Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
title_sort	longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9686ce98b6f64f4faf7617ce8e795119
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Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals

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