Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley

Abstract The SQUAMOSA-promoter binding like (SPL) gene family encodes transcription factors that have been shown in many species to influence plant growth and development, but information about these genes in barley (Hordeum vulgare L.) is limited. This study identified 17 barley SPL genes, within e...

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Autores principales:	Rajiv K. Tripathi, Phil Bregitzer, Jaswinder Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9690d96af8d14e0b956af7c1e12cca53
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spelling	oai:doaj.org-article:9690d96af8d14e0b956af7c1e12cca532021-12-02T15:08:33ZGenome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley10.1038/s41598-018-25349-02045-2322https://doaj.org/article/9690d96af8d14e0b956af7c1e12cca532018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25349-0https://doaj.org/toc/2045-2322Abstract The SQUAMOSA-promoter binding like (SPL) gene family encodes transcription factors that have been shown in many species to influence plant growth and development, but information about these genes in barley (Hordeum vulgare L.) is limited. This study identified 17 barley SPL genes, within eight distinct groups, that are orthologs of SPL genes described in Arabidopsis, wheat, and rice. Sixteen barley SPLs undergo alternative splicing. Seven SPLs contain a putative miR156 target site and the transcript levels of the miR156-targeted HvSPLs (HvSPL3, 13 and 23) were lower in vegetative than in reproductive phase but this was true also for some SPL genes such as HvSPL6 that were not regulated by miR156. Because SPL gene products regulate miR172, which is also involved in floral development, the expression of miR172 was studied. An antagonistic expression pattern of miR156 and miR172b during the vegetative and the reproductive phases signifies their apparent function in barley growth phase transition. Characterization of a barley mir172 mutant having an abnormal, indeterminate spikelet phenotype suggests the possible feedback role of AP2/miR172 module on HvSPL genes. This is the first comprehensive analysis of the miR156/SPL/miR172 axis in barley that provides a basis to elucidate their roles in various biological processes.Rajiv K. TripathiPhil BregitzerJaswinder SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajiv K. Tripathi Phil Bregitzer Jaswinder Singh Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
description	Abstract The SQUAMOSA-promoter binding like (SPL) gene family encodes transcription factors that have been shown in many species to influence plant growth and development, but information about these genes in barley (Hordeum vulgare L.) is limited. This study identified 17 barley SPL genes, within eight distinct groups, that are orthologs of SPL genes described in Arabidopsis, wheat, and rice. Sixteen barley SPLs undergo alternative splicing. Seven SPLs contain a putative miR156 target site and the transcript levels of the miR156-targeted HvSPLs (HvSPL3, 13 and 23) were lower in vegetative than in reproductive phase but this was true also for some SPL genes such as HvSPL6 that were not regulated by miR156. Because SPL gene products regulate miR172, which is also involved in floral development, the expression of miR172 was studied. An antagonistic expression pattern of miR156 and miR172b during the vegetative and the reproductive phases signifies their apparent function in barley growth phase transition. Characterization of a barley mir172 mutant having an abnormal, indeterminate spikelet phenotype suggests the possible feedback role of AP2/miR172 module on HvSPL genes. This is the first comprehensive analysis of the miR156/SPL/miR172 axis in barley that provides a basis to elucidate their roles in various biological processes.
format	article
author	Rajiv K. Tripathi Phil Bregitzer Jaswinder Singh
author_facet	Rajiv K. Tripathi Phil Bregitzer Jaswinder Singh
author_sort	Rajiv K. Tripathi
title	Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
title_short	Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
title_full	Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
title_fullStr	Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
title_full_unstemmed	Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
title_sort	genome-wide analysis of the spl/mir156 module and its interaction with the ap2/mir172 unit in barley
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9690d96af8d14e0b956af7c1e12cca53
work_keys_str_mv	AT rajivktripathi genomewideanalysisofthesplmir156moduleanditsinteractionwiththeap2mir172unitinbarley AT philbregitzer genomewideanalysisofthesplmir156moduleanditsinteractionwiththeap2mir172unitinbarley AT jaswindersingh genomewideanalysisofthesplmir156moduleanditsinteractionwiththeap2mir172unitinbarley
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Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley

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