Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect

The lack of a sufficient research base is the reason for the ongoing discussion regarding the genotoxic effect of magnetic field (MF) exposure on mammalian cell cultures. Chinese hamster ovary (CHO) suspension-type cells, which are widely used for biopharmaceutical production, are potentially subjec...

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Autores principales:	Alina Rekena, Dora Livkisa, Edmunds Kamolins, Juris Vanags, Dagnija Loca
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Chinese hamster ovary cells static magnetic field magnetically coupled stirring bioreactors biopharmaceuticals genotoxicity Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/9692bf3567ff4cf1a0dbe448dba0fff2
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spelling	oai:doaj.org-article:9692bf3567ff4cf1a0dbe448dba0fff22021-12-01T02:19:18ZBiopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect2296-418510.3389/fbioe.2021.751538https://doaj.org/article/9692bf3567ff4cf1a0dbe448dba0fff22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.751538/fullhttps://doaj.org/toc/2296-4185The lack of a sufficient research base is the reason for the ongoing discussion regarding the genotoxic effect of magnetic field (MF) exposure on mammalian cell cultures. Chinese hamster ovary (CHO) suspension-type cells, which are widely used for biopharmaceutical production, are potentially subjected to an increased MF when cultivated in bioreactors equipped with bottom-placed magnetically coupled stirring mechanisms. The main challenge for conducting research in this field remains the availability of a suitable experimental setup that generates an appropriate MF for the raised research question. In the present study, a simple and cost-effective experimental setup was developed that generated a static MF, similar to what has been modeled in large-scale bioreactors and, at the same time, was suitable for experimental cell cultivation in laboratory conditions. The measured maximum magnetic flux density to which the cells were exposed was 0.66 T. To assess the possible genotoxic effect, cells were continuously subcultivated in laboratory petri dishes for a period of 14 days, corresponding to a typical duration of a biopharmaceutical production process in a conventional fed-batch regime. The genotoxic effect was assessed using the cytokinesis-block micronucleus assay with fluorescent staining. Results showed that a 0.66-T static MF exposure had no significant long-term effect on cell viability and chromosomal damage but demonstrated a short-term effect on cell apoptosis. Significant increase in nuclear bud formation was observed. These findings may encourage other researchers in future studies investigating cellular responses to MF exposure and contribute relevant data for comparison.Alina RekenaDora LivkisaEdmunds KamolinsEdmunds KamolinsJuris VanagsDagnija LocaDagnija LocaFrontiers Media S.A.articleChinese hamster ovary cellsstatic magnetic fieldmagnetically coupled stirringbioreactorsbiopharmaceuticalsgenotoxicityBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chinese hamster ovary cells static magnetic field magnetically coupled stirring bioreactors biopharmaceuticals genotoxicity Biotechnology TP248.13-248.65
spellingShingle	Chinese hamster ovary cells static magnetic field magnetically coupled stirring bioreactors biopharmaceuticals genotoxicity Biotechnology TP248.13-248.65 Alina Rekena Dora Livkisa Edmunds Kamolins Edmunds Kamolins Juris Vanags Dagnija Loca Dagnija Loca Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
description	The lack of a sufficient research base is the reason for the ongoing discussion regarding the genotoxic effect of magnetic field (MF) exposure on mammalian cell cultures. Chinese hamster ovary (CHO) suspension-type cells, which are widely used for biopharmaceutical production, are potentially subjected to an increased MF when cultivated in bioreactors equipped with bottom-placed magnetically coupled stirring mechanisms. The main challenge for conducting research in this field remains the availability of a suitable experimental setup that generates an appropriate MF for the raised research question. In the present study, a simple and cost-effective experimental setup was developed that generated a static MF, similar to what has been modeled in large-scale bioreactors and, at the same time, was suitable for experimental cell cultivation in laboratory conditions. The measured maximum magnetic flux density to which the cells were exposed was 0.66 T. To assess the possible genotoxic effect, cells were continuously subcultivated in laboratory petri dishes for a period of 14 days, corresponding to a typical duration of a biopharmaceutical production process in a conventional fed-batch regime. The genotoxic effect was assessed using the cytokinesis-block micronucleus assay with fluorescent staining. Results showed that a 0.66-T static MF exposure had no significant long-term effect on cell viability and chromosomal damage but demonstrated a short-term effect on cell apoptosis. Significant increase in nuclear bud formation was observed. These findings may encourage other researchers in future studies investigating cellular responses to MF exposure and contribute relevant data for comparison.
format	article
author	Alina Rekena Dora Livkisa Edmunds Kamolins Edmunds Kamolins Juris Vanags Dagnija Loca Dagnija Loca
author_facet	Alina Rekena Dora Livkisa Edmunds Kamolins Edmunds Kamolins Juris Vanags Dagnija Loca Dagnija Loca
author_sort	Alina Rekena
title	Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
title_short	Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
title_full	Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
title_fullStr	Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
title_full_unstemmed	Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
title_sort	biopharmaceutical-type chinese hamster ovary cell cultivation under static magnetic field exposure: a study of genotoxic effect
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9692bf3567ff4cf1a0dbe448dba0fff2
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Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect

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