Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.

We present the first demonstration of glycated albumin detection and quantification using Raman spectroscopy without the addition of reagents. Glycated albumin is an important marker for monitoring the long-term glycemic history of diabetics, especially as its concentrations, in contrast to glycated...

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Autores principales:	Narahara Chari Dingari, Gary L Horowitz, Jeon Woong Kang, Ramachandra R Dasari, Ishan Barman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96937254673d436eb47fa24f7e27710f
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spelling	oai:doaj.org-article:96937254673d436eb47fa24f7e27710f2021-12-02T20:18:59ZRaman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.1932-620310.1371/journal.pone.0032406https://doaj.org/article/96937254673d436eb47fa24f7e27710f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22393405/?tool=EBIhttps://doaj.org/toc/1932-6203We present the first demonstration of glycated albumin detection and quantification using Raman spectroscopy without the addition of reagents. Glycated albumin is an important marker for monitoring the long-term glycemic history of diabetics, especially as its concentrations, in contrast to glycated hemoglobin levels, are unaffected by changes in erythrocyte life times. Clinically, glycated albumin concentrations show a strong correlation with the development of serious diabetes complications including nephropathy and retinopathy. In this article, we propose and evaluate the efficacy of Raman spectroscopy for determination of this important analyte. By utilizing the pre-concentration obtained through drop-coating deposition, we show that glycation of albumin leads to subtle, but consistent, changes in vibrational features, which with the help of multivariate classification techniques can be used to discriminate glycated albumin from the unglycated variant with 100% accuracy. Moreover, we demonstrate that the calibration model developed on the glycated albumin spectral dataset shows high predictive power, even at substantially lower concentrations than those typically encountered in clinical practice. In fact, the limit of detection for glycated albumin measurements is calculated to be approximately four times lower than its minimum physiological concentration. Importantly, in relation to the existing detection methods for glycated albumin, the proposed method is also completely reagent-free, requires barely any sample preparation and has the potential for simultaneous determination of glycated hemoglobin levels as well. Given these key advantages, we believe that the proposed approach can provide a uniquely powerful tool for quantification of glycation status of proteins in biopharmaceutical development as well as for glycemic marker determination in routine clinical diagnostics in the future.Narahara Chari DingariGary L HorowitzJeon Woong KangRamachandra R DasariIshan BarmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e32406 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Narahara Chari Dingari Gary L Horowitz Jeon Woong Kang Ramachandra R Dasari Ishan Barman Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
description	We present the first demonstration of glycated albumin detection and quantification using Raman spectroscopy without the addition of reagents. Glycated albumin is an important marker for monitoring the long-term glycemic history of diabetics, especially as its concentrations, in contrast to glycated hemoglobin levels, are unaffected by changes in erythrocyte life times. Clinically, glycated albumin concentrations show a strong correlation with the development of serious diabetes complications including nephropathy and retinopathy. In this article, we propose and evaluate the efficacy of Raman spectroscopy for determination of this important analyte. By utilizing the pre-concentration obtained through drop-coating deposition, we show that glycation of albumin leads to subtle, but consistent, changes in vibrational features, which with the help of multivariate classification techniques can be used to discriminate glycated albumin from the unglycated variant with 100% accuracy. Moreover, we demonstrate that the calibration model developed on the glycated albumin spectral dataset shows high predictive power, even at substantially lower concentrations than those typically encountered in clinical practice. In fact, the limit of detection for glycated albumin measurements is calculated to be approximately four times lower than its minimum physiological concentration. Importantly, in relation to the existing detection methods for glycated albumin, the proposed method is also completely reagent-free, requires barely any sample preparation and has the potential for simultaneous determination of glycated hemoglobin levels as well. Given these key advantages, we believe that the proposed approach can provide a uniquely powerful tool for quantification of glycation status of proteins in biopharmaceutical development as well as for glycemic marker determination in routine clinical diagnostics in the future.
format	article
author	Narahara Chari Dingari Gary L Horowitz Jeon Woong Kang Ramachandra R Dasari Ishan Barman
author_facet	Narahara Chari Dingari Gary L Horowitz Jeon Woong Kang Ramachandra R Dasari Ishan Barman
author_sort	Narahara Chari Dingari
title	Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
title_short	Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
title_full	Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
title_fullStr	Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
title_full_unstemmed	Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
title_sort	raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/96937254673d436eb47fa24f7e27710f
work_keys_str_mv	AT naraharacharidingari ramanspectroscopyprovidesapowerfuldiagnostictoolforaccuratedeterminationofalbuminglycation AT garylhorowitz ramanspectroscopyprovidesapowerfuldiagnostictoolforaccuratedeterminationofalbuminglycation AT jeonwoongkang ramanspectroscopyprovidesapowerfuldiagnostictoolforaccuratedeterminationofalbuminglycation AT ramachandrardasari ramanspectroscopyprovidesapowerfuldiagnostictoolforaccuratedeterminationofalbuminglycation AT ishanbarman ramanspectroscopyprovidesapowerfuldiagnostictoolforaccuratedeterminationofalbuminglycation
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Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.

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