Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance

Abstract Plants have evolved genetic and physiological mechanisms to mitigate the adverse effects of high temperature. CARBOXYL TERMINUS OF THE HSC70-INTERACTING PROTEINS (CHIP) is a conserved chaperone-dependent ubiquitin E3 ligase that targets misfolded proteins. Here, we report functional analysi...

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Autores principales:	Yan Zhang, Xiaodong Lai, Siqing Yang, Huan Ren, Jingya Yuan, Huanchun Jin, Chengchen Shi, Zhibing Lai, Gengshou Xia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9693c4d9212d4b6c881ab4009917dc7f
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spelling	oai:doaj.org-article:9693c4d9212d4b6c881ab4009917dc7f2021-12-02T10:49:16ZFunctional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance10.1038/s41598-021-81372-82045-2322https://doaj.org/article/9693c4d9212d4b6c881ab4009917dc7f2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81372-8https://doaj.org/toc/2045-2322Abstract Plants have evolved genetic and physiological mechanisms to mitigate the adverse effects of high temperature. CARBOXYL TERMINUS OF THE HSC70-INTERACTING PROTEINS (CHIP) is a conserved chaperone-dependent ubiquitin E3 ligase that targets misfolded proteins. Here, we report functional analysis of the SlCHIP gene from tomato (Solanum lycopersicum) in heat tolerance. SlCHIP encodes a CHIP protein with three tandem tetracopeptide repeat (TPR) motifs and a C-terminal U box domain. Phylogenetic analysis of CHIP homologs from animals, spore-bearing and seed plants revealed a tree topology similar to the evolutionary tree of the organisms. Expression of SlCHIP was induced under high temperature and was also responsive to plant stress hormones. Silencing of SlCHIP in tomato reduced heat tolerance based on increased heat stress symptoms, reduced photosynthetic activity, elevated electrolyte leakage and accumulation of insoluble protein aggregates. The accumulated protein aggregates in SlCHIP-silenced plants were still highly ubiquitinated, suggesting involvement of other E3 ligases in ubiquitination. SlCHIP restored the heat tolerance of Arabidopsis chip mutant to the wild type levels. These results indicate that tomato SlCHIP plays a critical role in heat stress responses most likely by targeting degradation of misfolded proteins that are generated during heat stress.Yan ZhangXiaodong LaiSiqing YangHuan RenJingya YuanHuanchun JinChengchen ShiZhibing LaiGengshou XiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Zhang Xiaodong Lai Siqing Yang Huan Ren Jingya Yuan Huanchun Jin Chengchen Shi Zhibing Lai Gengshou Xia Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
description	Abstract Plants have evolved genetic and physiological mechanisms to mitigate the adverse effects of high temperature. CARBOXYL TERMINUS OF THE HSC70-INTERACTING PROTEINS (CHIP) is a conserved chaperone-dependent ubiquitin E3 ligase that targets misfolded proteins. Here, we report functional analysis of the SlCHIP gene from tomato (Solanum lycopersicum) in heat tolerance. SlCHIP encodes a CHIP protein with three tandem tetracopeptide repeat (TPR) motifs and a C-terminal U box domain. Phylogenetic analysis of CHIP homologs from animals, spore-bearing and seed plants revealed a tree topology similar to the evolutionary tree of the organisms. Expression of SlCHIP was induced under high temperature and was also responsive to plant stress hormones. Silencing of SlCHIP in tomato reduced heat tolerance based on increased heat stress symptoms, reduced photosynthetic activity, elevated electrolyte leakage and accumulation of insoluble protein aggregates. The accumulated protein aggregates in SlCHIP-silenced plants were still highly ubiquitinated, suggesting involvement of other E3 ligases in ubiquitination. SlCHIP restored the heat tolerance of Arabidopsis chip mutant to the wild type levels. These results indicate that tomato SlCHIP plays a critical role in heat stress responses most likely by targeting degradation of misfolded proteins that are generated during heat stress.
format	article
author	Yan Zhang Xiaodong Lai Siqing Yang Huan Ren Jingya Yuan Huanchun Jin Chengchen Shi Zhibing Lai Gengshou Xia
author_facet	Yan Zhang Xiaodong Lai Siqing Yang Huan Ren Jingya Yuan Huanchun Jin Chengchen Shi Zhibing Lai Gengshou Xia
author_sort	Yan Zhang
title	Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
title_short	Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
title_full	Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
title_fullStr	Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
title_full_unstemmed	Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
title_sort	functional analysis of tomato chip ubiquitin e3 ligase in heat tolerance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9693c4d9212d4b6c881ab4009917dc7f
work_keys_str_mv	AT yanzhang functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT xiaodonglai functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT siqingyang functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT huanren functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT jingyayuan functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT huanchunjin functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT chengchenshi functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT zhibinglai functionalanalysisoftomatochipubiquitine3ligaseinheattolerance AT gengshouxia functionalanalysisoftomatochipubiquitine3ligaseinheattolerance
_version_	1718396607041372160

Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance

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