Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation

Abstract CircRNAs, a class of widespread circular RNAs produced from precursor mRNA back-splicing, have been implicated in regulation of gene expression in eukaryotes, but their biological functions in plants have not yet been elucidated. By deep sequencing of rRNA-removed and RNase R-digested RNA s...

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Autores principales:	Jinjuan Tan, Zhongjing Zhou, Yujie Niu, Xiaoyong Sun, Zhiping Deng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/969a9907a02a4ec1b212364059c854a1
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spelling	oai:doaj.org-article:969a9907a02a4ec1b212364059c854a12021-12-02T15:06:13ZIdentification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation10.1038/s41598-017-08806-02045-2322https://doaj.org/article/969a9907a02a4ec1b212364059c854a12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08806-0https://doaj.org/toc/2045-2322Abstract CircRNAs, a class of widespread circular RNAs produced from precursor mRNA back-splicing, have been implicated in regulation of gene expression in eukaryotes, but their biological functions in plants have not yet been elucidated. By deep sequencing of rRNA-removed and RNase R-digested RNA samples we have identified several thousands of putative back-splicing sites in tomato fruit (Solanum lycopersicum) and show that the abundance of some of these circRNAs derived from fruit pigment biosynthesis genes are regulated by fruit ripening. Herein, we overexpressed a circRNA derived from Phytoene Synthase 1 (PSY1) in tomato ‘Ailsa Craig’ and microTom. The PSY1 mRNA abundance, the lycopene and β-carotene accumulation were decreased significantly in the transgenic tomato fruits, likely due to the continuous highly expressed circRNAs and/or the low abundant linear RNAs generated from the overexpression vector. Besides, overexpression of a circRNA derived from Phytoene Desaturase (PDS) showed similar results. Our results provide biological insights into plant circRNAs.Jinjuan TanZhongjing ZhouYujie NiuXiaoyong SunZhiping DengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinjuan Tan Zhongjing Zhou Yujie Niu Xiaoyong Sun Zhiping Deng Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation
description	Abstract CircRNAs, a class of widespread circular RNAs produced from precursor mRNA back-splicing, have been implicated in regulation of gene expression in eukaryotes, but their biological functions in plants have not yet been elucidated. By deep sequencing of rRNA-removed and RNase R-digested RNA samples we have identified several thousands of putative back-splicing sites in tomato fruit (Solanum lycopersicum) and show that the abundance of some of these circRNAs derived from fruit pigment biosynthesis genes are regulated by fruit ripening. Herein, we overexpressed a circRNA derived from Phytoene Synthase 1 (PSY1) in tomato ‘Ailsa Craig’ and microTom. The PSY1 mRNA abundance, the lycopene and β-carotene accumulation were decreased significantly in the transgenic tomato fruits, likely due to the continuous highly expressed circRNAs and/or the low abundant linear RNAs generated from the overexpression vector. Besides, overexpression of a circRNA derived from Phytoene Desaturase (PDS) showed similar results. Our results provide biological insights into plant circRNAs.
format	article
author	Jinjuan Tan Zhongjing Zhou Yujie Niu Xiaoyong Sun Zhiping Deng
author_facet	Jinjuan Tan Zhongjing Zhou Yujie Niu Xiaoyong Sun Zhiping Deng
author_sort	Jinjuan Tan
title	Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation
title_short	Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation
title_full	Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation
title_fullStr	Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation
title_full_unstemmed	Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation
title_sort	identification and functional characterization of tomato circrnas derived from genes involved in fruit pigment accumulation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/969a9907a02a4ec1b212364059c854a1
work_keys_str_mv	AT jinjuantan identificationandfunctionalcharacterizationoftomatocircrnasderivedfromgenesinvolvedinfruitpigmentaccumulation AT zhongjingzhou identificationandfunctionalcharacterizationoftomatocircrnasderivedfromgenesinvolvedinfruitpigmentaccumulation AT yujieniu identificationandfunctionalcharacterizationoftomatocircrnasderivedfromgenesinvolvedinfruitpigmentaccumulation AT xiaoyongsun identificationandfunctionalcharacterizationoftomatocircrnasderivedfromgenesinvolvedinfruitpigmentaccumulation AT zhipingdeng identificationandfunctionalcharacterizationoftomatocircrnasderivedfromgenesinvolvedinfruitpigmentaccumulation
_version_	1718388529854152704

Identification and Functional Characterization of Tomato CircRNAs Derived from Genes Involved in Fruit Pigment Accumulation

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