Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response

Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response

Abstract Millimeter wave (MMW)-induced heating represents a promising alternative for non-invasive hyperthermia of superficial skin cancer, such as melanoma. Pulsed MMW-induced heating of tumors allows for reaching high peak temperatures without overheating surrounding tissues. Herein, for the first...

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Autores principales: Rosa Orlacchio, Yann Le Page, Yves Le Dréan, Rémy Le Guével, Ronan Sauleau, Stanislav Alekseev, Maxim Zhadobov
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Publicado: Nature Portfolio 2019
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spelling oai:doaj.org-article:969c5cf20f594b18bb6bf227b30323fa2021-12-02T15:07:53ZMillimeter-wave pulsed heating in vitro: cell mortality and heat shock response10.1038/s41598-019-51731-72045-2322https://doaj.org/article/969c5cf20f594b18bb6bf227b30323fa2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51731-7https://doaj.org/toc/2045-2322Abstract Millimeter wave (MMW)-induced heating represents a promising alternative for non-invasive hyperthermia of superficial skin cancer, such as melanoma. Pulsed MMW-induced heating of tumors allows for reaching high peak temperatures without overheating surrounding tissues. Herein, for the first time, we evaluate apoptotic and heat shock responses of melanoma cells exposed in vitro to continuous (CW) or pulsed-wave (PW) amplitude-modulated MMW at 58.4 GHz with the same average temperature rise. Using an ad hoc exposure system, we generated 90 min pulse train with 1.5 s pulse duration, period of 20 s, amplitude of 10 °C, and steady-state temperature at the level of cells of 49.2 °C. The activation of Caspase-3 and phosphorylation of HSP27 were investigated using fluorescence microscopy to monitor the spatial variation of cellular response. Our results demonstrate that, under the considered exposure conditions, Caspase-3 activation was almost 5 times greater following PW exposure compared to CW. The relationship between the PW-induced cellular response and SAR-dependent temperature rise was non-linear. Phosphorylation of HSP27 was 58% stronger for PW compared to CW. It exhibits a plateau for the peak temperature ranging from 47.7 to 49.2 °C. Our results provide an insight into understanding of the cellular response to MMW-induced pulsed heating.Rosa OrlacchioYann Le PageYves Le DréanRémy Le GuévelRonan SauleauStanislav AlekseevMaxim ZhadobovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Rosa Orlacchio
Yann Le Page
Yves Le Dréan
Rémy Le Guével
Ronan Sauleau
Stanislav Alekseev
Maxim Zhadobov
Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
description Abstract Millimeter wave (MMW)-induced heating represents a promising alternative for non-invasive hyperthermia of superficial skin cancer, such as melanoma. Pulsed MMW-induced heating of tumors allows for reaching high peak temperatures without overheating surrounding tissues. Herein, for the first time, we evaluate apoptotic and heat shock responses of melanoma cells exposed in vitro to continuous (CW) or pulsed-wave (PW) amplitude-modulated MMW at 58.4 GHz with the same average temperature rise. Using an ad hoc exposure system, we generated 90 min pulse train with 1.5 s pulse duration, period of 20 s, amplitude of 10 °C, and steady-state temperature at the level of cells of 49.2 °C. The activation of Caspase-3 and phosphorylation of HSP27 were investigated using fluorescence microscopy to monitor the spatial variation of cellular response. Our results demonstrate that, under the considered exposure conditions, Caspase-3 activation was almost 5 times greater following PW exposure compared to CW. The relationship between the PW-induced cellular response and SAR-dependent temperature rise was non-linear. Phosphorylation of HSP27 was 58% stronger for PW compared to CW. It exhibits a plateau for the peak temperature ranging from 47.7 to 49.2 °C. Our results provide an insight into understanding of the cellular response to MMW-induced pulsed heating.
format article
author Rosa Orlacchio
Yann Le Page
Yves Le Dréan
Rémy Le Guével
Ronan Sauleau
Stanislav Alekseev
Maxim Zhadobov
author_facet Rosa Orlacchio
Yann Le Page
Yves Le Dréan
Rémy Le Guével
Ronan Sauleau
Stanislav Alekseev
Maxim Zhadobov
author_sort Rosa Orlacchio
title Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
title_short Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
title_full Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
title_fullStr Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
title_full_unstemmed Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
title_sort millimeter-wave pulsed heating in vitro: cell mortality and heat shock response
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/969c5cf20f594b18bb6bf227b30323fa
work_keys_str_mv AT rosaorlacchio millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
AT yannlepage millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
AT yvesledrean millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
AT remyleguevel millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
AT ronansauleau millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
AT stanislavalekseev millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
AT maximzhadobov millimeterwavepulsedheatinginvitrocellmortalityandheatshockresponse
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