Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station

ABSTRACT The initial characterization of the Aspergillus niger isolate JSC-093350089, collected from U.S. segment surfaces of the International Space Station (ISS), is reported, along with a comparison to the extensively studied strain ATCC 1015. Whole-genome sequencing of the ISS isolate enabled it...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jillian Romsdahl, Adriana Blachowicz, Abby J. Chiang, Nitin Singh, Jason E. Stajich, Markus Kalkum, Kasthuri Venkateswaran, Clay C. C. Wang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Aspergillus niger International Space Station phylogenetic analysis proteomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/96a12aa2ec2e439cb36dd8b94a79e0d9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:96a12aa2ec2e439cb36dd8b94a79e0d9
record_format	dspace
spelling	oai:doaj.org-article:96a12aa2ec2e439cb36dd8b94a79e0d92021-12-02T19:47:34ZCharacterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station10.1128/mSystems.00112-182379-5077https://doaj.org/article/96a12aa2ec2e439cb36dd8b94a79e0d92018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00112-18https://doaj.org/toc/2379-5077ABSTRACT The initial characterization of the Aspergillus niger isolate JSC-093350089, collected from U.S. segment surfaces of the International Space Station (ISS), is reported, along with a comparison to the extensively studied strain ATCC 1015. Whole-genome sequencing of the ISS isolate enabled its phylogenetic placement within the A. niger/welwitschiae/lacticoffeatus clade and revealed that the genome of JSC-093350089 is within the observed genetic variance of other sequenced A. niger strains. The ISS isolate exhibited an increased rate of growth and pigment distribution compared to a terrestrial strain. Analysis of the isolate’s proteome revealed significant differences in the molecular phenotype of JSC-093350089, including increased abundance of proteins involved in the A. niger starvation response, oxidative stress resistance, cell wall modulation, and nutrient acquisition. Together, these data reveal the existence of a distinct strain of A. niger on board the ISS and provide insight into the characteristics of melanized fungal species inhabiting spacecraft environments. IMPORTANCE A thorough understanding of how fungi respond and adapt to the various stimuli encountered during spaceflight presents many economic benefits and is imperative for the health of crew. As A. niger is a predominant ISS isolate frequently detected in built environments, studies of A. niger strains inhabiting closed systems may reveal information fundamental to the success of long-duration space missions. This investigation provides valuable insights into the adaptive mechanisms of fungi in extreme environments as well as countermeasures to eradicate unfavorable microbes. Further, it enhances understanding of host-microbe interactions in closed systems, which can help NASA’s Human Research Program maintain a habitat healthy for crew during long-term manned space missions.Jillian RomsdahlAdriana BlachowiczAbby J. ChiangNitin SinghJason E. StajichMarkus KalkumKasthuri VenkateswaranClay C. C. WangAmerican Society for MicrobiologyarticleAspergillus nigerInternational Space Stationphylogenetic analysisproteomicsMicrobiologyQR1-502ENmSystems, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aspergillus niger International Space Station phylogenetic analysis proteomics Microbiology QR1-502
spellingShingle	Aspergillus niger International Space Station phylogenetic analysis proteomics Microbiology QR1-502 Jillian Romsdahl Adriana Blachowicz Abby J. Chiang Nitin Singh Jason E. Stajich Markus Kalkum Kasthuri Venkateswaran Clay C. C. Wang Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station
description	ABSTRACT The initial characterization of the Aspergillus niger isolate JSC-093350089, collected from U.S. segment surfaces of the International Space Station (ISS), is reported, along with a comparison to the extensively studied strain ATCC 1015. Whole-genome sequencing of the ISS isolate enabled its phylogenetic placement within the A. niger/welwitschiae/lacticoffeatus clade and revealed that the genome of JSC-093350089 is within the observed genetic variance of other sequenced A. niger strains. The ISS isolate exhibited an increased rate of growth and pigment distribution compared to a terrestrial strain. Analysis of the isolate’s proteome revealed significant differences in the molecular phenotype of JSC-093350089, including increased abundance of proteins involved in the A. niger starvation response, oxidative stress resistance, cell wall modulation, and nutrient acquisition. Together, these data reveal the existence of a distinct strain of A. niger on board the ISS and provide insight into the characteristics of melanized fungal species inhabiting spacecraft environments. IMPORTANCE A thorough understanding of how fungi respond and adapt to the various stimuli encountered during spaceflight presents many economic benefits and is imperative for the health of crew. As A. niger is a predominant ISS isolate frequently detected in built environments, studies of A. niger strains inhabiting closed systems may reveal information fundamental to the success of long-duration space missions. This investigation provides valuable insights into the adaptive mechanisms of fungi in extreme environments as well as countermeasures to eradicate unfavorable microbes. Further, it enhances understanding of host-microbe interactions in closed systems, which can help NASA’s Human Research Program maintain a habitat healthy for crew during long-term manned space missions.
format	article
author	Jillian Romsdahl Adriana Blachowicz Abby J. Chiang Nitin Singh Jason E. Stajich Markus Kalkum Kasthuri Venkateswaran Clay C. C. Wang
author_facet	Jillian Romsdahl Adriana Blachowicz Abby J. Chiang Nitin Singh Jason E. Stajich Markus Kalkum Kasthuri Venkateswaran Clay C. C. Wang
author_sort	Jillian Romsdahl
title	Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station
title_short	Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station
title_full	Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station
title_fullStr	Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station
title_full_unstemmed	Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station
title_sort	characterization of <named-content content-type="genus-species">aspergillus niger</named-content> isolated from the international space station
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/96a12aa2ec2e439cb36dd8b94a79e0d9
work_keys_str_mv	AT jillianromsdahl characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT adrianablachowicz characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT abbyjchiang characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT nitinsingh characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT jasonestajich characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT markuskalkum characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT kasthurivenkateswaran characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation AT clayccwang characterizationofnamedcontentcontenttypegenusspeciesaspergillusnigernamedcontentisolatedfromtheinternationalspacestation
_version_	1718375976100954112

Characterization of <named-content content-type="genus-species">Aspergillus niger</named-content> Isolated from the International Space Station

Ejemplares similares