Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.

Of the few preserved areas in the northeast of United States, the soil in the Pine Barrens Forests presents a harsh environment for the microorganisms to grow and survive. In the current study we report the use of clustering methods to scientifically select the sampling locations that would represen...

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Autores principales: Vishal Shah, Shreya Shah, Murty S Kambhampati, Jeffery Ambrose, Nyesha Smith, Scot E Dowd, Kevin T McDonnell, Bishnu Panigrahi, Timothy Green
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Publicado: Public Library of Science (PLoS) 2011
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Acceso en línea:https://doaj.org/article/96c0d55ddc38456b9d39a9d5386873bf
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spelling oai:doaj.org-article:96c0d55ddc38456b9d39a9d5386873bf2021-11-18T07:36:02ZBacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.1932-620310.1371/journal.pone.0026263https://doaj.org/article/96c0d55ddc38456b9d39a9d5386873bf2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22028845/?tool=EBIhttps://doaj.org/toc/1932-6203Of the few preserved areas in the northeast of United States, the soil in the Pine Barrens Forests presents a harsh environment for the microorganisms to grow and survive. In the current study we report the use of clustering methods to scientifically select the sampling locations that would represent the entire forest and also report the microbial diversity present in various horizons of the soil. Sixty six sampling locations were selected across the forest and soils were collected from three horizons (sampling depths). The three horizons were 0-10 cm (Horizon O); 11-25 cm (Horizon A) and 26-40 cm (Horizon B). Based on the total microbial substrate utilization pattern and K-means clustering analysis, the soil in the Pine Barrens Forest can be classified into four distinct clusters at each of the three horizons. One soil sample from each of the four clusters were selected and archaeal and bacterial populations within the soil studied using pyrosequencing method. The results show the microbial communities present in each of these clusters are different. Within the microbial communities present, microorganisms involved in nitrogen cycle occupy a major fraction of microbial community in the soil. High level of diversity was observed for nitrogen fixing bacteria. In contrast, Nitrosovibrio and Nitrosocaldus spp are the single bacterial and archaeal population respectively carrying out ammonia oxidation in the soil.Vishal ShahShreya ShahMurty S KambhampatiJeffery AmbroseNyesha SmithScot E DowdKevin T McDonnellBishnu PanigrahiTimothy GreenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26263 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Vishal Shah
Shreya Shah
Murty S Kambhampati
Jeffery Ambrose
Nyesha Smith
Scot E Dowd
Kevin T McDonnell
Bishnu Panigrahi
Timothy Green
Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.
description Of the few preserved areas in the northeast of United States, the soil in the Pine Barrens Forests presents a harsh environment for the microorganisms to grow and survive. In the current study we report the use of clustering methods to scientifically select the sampling locations that would represent the entire forest and also report the microbial diversity present in various horizons of the soil. Sixty six sampling locations were selected across the forest and soils were collected from three horizons (sampling depths). The three horizons were 0-10 cm (Horizon O); 11-25 cm (Horizon A) and 26-40 cm (Horizon B). Based on the total microbial substrate utilization pattern and K-means clustering analysis, the soil in the Pine Barrens Forest can be classified into four distinct clusters at each of the three horizons. One soil sample from each of the four clusters were selected and archaeal and bacterial populations within the soil studied using pyrosequencing method. The results show the microbial communities present in each of these clusters are different. Within the microbial communities present, microorganisms involved in nitrogen cycle occupy a major fraction of microbial community in the soil. High level of diversity was observed for nitrogen fixing bacteria. In contrast, Nitrosovibrio and Nitrosocaldus spp are the single bacterial and archaeal population respectively carrying out ammonia oxidation in the soil.
format article
author Vishal Shah
Shreya Shah
Murty S Kambhampati
Jeffery Ambrose
Nyesha Smith
Scot E Dowd
Kevin T McDonnell
Bishnu Panigrahi
Timothy Green
author_facet Vishal Shah
Shreya Shah
Murty S Kambhampati
Jeffery Ambrose
Nyesha Smith
Scot E Dowd
Kevin T McDonnell
Bishnu Panigrahi
Timothy Green
author_sort Vishal Shah
title Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.
title_short Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.
title_full Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.
title_fullStr Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.
title_full_unstemmed Bacterial and archaea community present in the Pine Barrens Forest of Long Island, NY: unusually high percentage of ammonia oxidizing bacteria.
title_sort bacterial and archaea community present in the pine barrens forest of long island, ny: unusually high percentage of ammonia oxidizing bacteria.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/96c0d55ddc38456b9d39a9d5386873bf
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