Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria

Objectives: The present study aimed to isolate potential antimicrobial and antioxidant Streptomyces sp from cave areas of Algeria. Methods: Streptomyces sp.GLD25 was identified by biochemical, physiological and molecular analysis. The antimicrobial activity has been carried out by well diffusion met...

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Autores principales:	Fatima Zohra Djebbah, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Larbi Belyagoubi, Farid Kherbouche, Djamel Eddine Abdelouahid, Balasubramani Ravindran
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Streptomyces Cave Antimicrobial Antioxidant GC–MS Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/96c7cb9de77446818ac18dbd1d9e2769
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spelling	oai:doaj.org-article:96c7cb9de77446818ac18dbd1d9e27692021-11-28T04:28:50ZIsolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria1018-364710.1016/j.jksus.2021.101719https://doaj.org/article/96c7cb9de77446818ac18dbd1d9e27692022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003815https://doaj.org/toc/1018-3647Objectives: The present study aimed to isolate potential antimicrobial and antioxidant Streptomyces sp from cave areas of Algeria. Methods: Streptomyces sp.GLD25 was identified by biochemical, physiological and molecular analysis. The antimicrobial activity has been carried out by well diffusion method, microplates method has been used to perform the MIC values of the organic extracts. The antioxidant capacity of the extract was tested by DPPH and FRAP methods. GC–MS analysis was used to study the chemical profile of the crude active extract. Results: The novel strain Streptomyces sp. GLD25 was isolated from Gueldaman cave in Algeria showed potential antioxidant and antimicrobial properties. According to the results of morphological, biochemical characteristics and complete 16S rRNA sequence, the strain revealed 99.71% of similarity with Streptomyces cyaneofuscatus strain WKFF71. The significant inhibition zone of the crude extract and MIC value were 30 mm and 0,015 mg/mL respectively against Staphylococcus aureus and Bacillus subtilis. In addition, the extract showed good antioxidant activity with IC50 = 20,12 mg/mL ± 2,05 for DPPH and IC50 = 8,80 mg/mL ± 0,2 for ferric reducing activity. The fundamental metabolites of the active extract were identified using GC–MS were Diisooctyl-phthalate, 6-hydroxy-heptanoic acid, Hexadecanoic acid, Benzeneacetic acid and 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionic acid. Conclusion: Overall, the Streptomyces sp.GLD25 isolated from Gueldamen cave have the potential to produce antimicrobials and antioxidants compounds with important biotechnological and medical applications.Fatima Zohra DjebbahNaif Abdullah Al-DhabiMariadhas Valan ArasuLarbi BelyagoubiFarid KherboucheDjamel Eddine AbdelouahidBalasubramani RavindranElsevierarticleStreptomycesCaveAntimicrobialAntioxidantGC–MSScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101719- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptomyces Cave Antimicrobial Antioxidant GC–MS Science (General) Q1-390
spellingShingle	Streptomyces Cave Antimicrobial Antioxidant GC–MS Science (General) Q1-390 Fatima Zohra Djebbah Naif Abdullah Al-Dhabi Mariadhas Valan Arasu Larbi Belyagoubi Farid Kherbouche Djamel Eddine Abdelouahid Balasubramani Ravindran Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
description	Objectives: The present study aimed to isolate potential antimicrobial and antioxidant Streptomyces sp from cave areas of Algeria. Methods: Streptomyces sp.GLD25 was identified by biochemical, physiological and molecular analysis. The antimicrobial activity has been carried out by well diffusion method, microplates method has been used to perform the MIC values of the organic extracts. The antioxidant capacity of the extract was tested by DPPH and FRAP methods. GC–MS analysis was used to study the chemical profile of the crude active extract. Results: The novel strain Streptomyces sp. GLD25 was isolated from Gueldaman cave in Algeria showed potential antioxidant and antimicrobial properties. According to the results of morphological, biochemical characteristics and complete 16S rRNA sequence, the strain revealed 99.71% of similarity with Streptomyces cyaneofuscatus strain WKFF71. The significant inhibition zone of the crude extract and MIC value were 30 mm and 0,015 mg/mL respectively against Staphylococcus aureus and Bacillus subtilis. In addition, the extract showed good antioxidant activity with IC50 = 20,12 mg/mL ± 2,05 for DPPH and IC50 = 8,80 mg/mL ± 0,2 for ferric reducing activity. The fundamental metabolites of the active extract were identified using GC–MS were Diisooctyl-phthalate, 6-hydroxy-heptanoic acid, Hexadecanoic acid, Benzeneacetic acid and 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionic acid. Conclusion: Overall, the Streptomyces sp.GLD25 isolated from Gueldamen cave have the potential to produce antimicrobials and antioxidants compounds with important biotechnological and medical applications.
format	article
author	Fatima Zohra Djebbah Naif Abdullah Al-Dhabi Mariadhas Valan Arasu Larbi Belyagoubi Farid Kherbouche Djamel Eddine Abdelouahid Balasubramani Ravindran
author_facet	Fatima Zohra Djebbah Naif Abdullah Al-Dhabi Mariadhas Valan Arasu Larbi Belyagoubi Farid Kherbouche Djamel Eddine Abdelouahid Balasubramani Ravindran
author_sort	Fatima Zohra Djebbah
title	Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
title_short	Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
title_full	Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
title_fullStr	Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
title_full_unstemmed	Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria
title_sort	isolation and characterisation of streptomyces sp. strain gld25 with antimicrobial and antioxidant effects from gueldaman cave (gld1), akbou-algeria
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/96c7cb9de77446818ac18dbd1d9e2769
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Isolation and characterisation of Streptomyces sp. Strain GLD25 with antimicrobial and antioxidant effects from Gueldaman cave (GLD1), Akbou-Algeria

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