Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression

This laboratory module simulates the process used by working scientists to ask and answer a question of biological interest. Instructors facilitate acquisition of knowledge using a comprehensive, inquiry-based approach in which students learn theory, hypothesis development, experimental design, and...

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Autores principales:	Kathryn E. Gunn, Christine Seitz McCauslin, Jennifer Staiger, Dana M. Pirone
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/96cd79083bed43f29288c3f30c089e9f
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spelling	oai:doaj.org-article:96cd79083bed43f29288c3f30c089e9f2021-11-15T15:18:41ZInquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression10.1128/jmbe.v14i2.5421935-78851935-7877https://doaj.org/article/96cd79083bed43f29288c3f30c089e9f2013-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v14i2.542https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885This laboratory module simulates the process used by working scientists to ask and answer a question of biological interest. Instructors facilitate acquisition of knowledge using a comprehensive, inquiry-based approach in which students learn theory, hypothesis development, experimental design, and data interpretation and presentation. Using inflammation in macrophages as a model system, students perform a series of molecular biology techniques to address the biological question: “Does stimulus ‘X’ induce inflammation?” To ask this question, macrophage cells are treated with putative inflammatory mediators and then assayed for evidence of inflammatory response. Students become familiar with their assigned mediator and the relationship between their mediator and inflammation by conducting literature searches, then using this information to generate hypotheses which address the effect of their mediator on induction of inflammation. The cellular and molecular approaches used to test their hypotheses include transfection and luciferase reporter assay, immunoblot, fluorescence microscopy, enzyme-linked immunosorbent assay, and quantitative PCR. Quantitative and qualitative reasoning skills are developed through data analysis and demonstrated by successful completion of post-lab worksheets and the generation and oral presentation of a scientific poster. Learning objective assessment relies on four instruments: pre-lab quizzes, post-lab worksheets, poster presentation, and posttest. Within three cohorts (n = 85) more than 95% of our students successfully achieved the learning objectives.Kathryn E. GunnChristine Seitz McCauslinJennifer StaigerDana M. PironeAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 14, Iss 2, Pp 189-196 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Kathryn E. Gunn Christine Seitz McCauslin Jennifer Staiger Dana M. Pirone Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression
description	This laboratory module simulates the process used by working scientists to ask and answer a question of biological interest. Instructors facilitate acquisition of knowledge using a comprehensive, inquiry-based approach in which students learn theory, hypothesis development, experimental design, and data interpretation and presentation. Using inflammation in macrophages as a model system, students perform a series of molecular biology techniques to address the biological question: “Does stimulus ‘X’ induce inflammation?” To ask this question, macrophage cells are treated with putative inflammatory mediators and then assayed for evidence of inflammatory response. Students become familiar with their assigned mediator and the relationship between their mediator and inflammation by conducting literature searches, then using this information to generate hypotheses which address the effect of their mediator on induction of inflammation. The cellular and molecular approaches used to test their hypotheses include transfection and luciferase reporter assay, immunoblot, fluorescence microscopy, enzyme-linked immunosorbent assay, and quantitative PCR. Quantitative and qualitative reasoning skills are developed through data analysis and demonstrated by successful completion of post-lab worksheets and the generation and oral presentation of a scientific poster. Learning objective assessment relies on four instruments: pre-lab quizzes, post-lab worksheets, poster presentation, and posttest. Within three cohorts (n = 85) more than 95% of our students successfully achieved the learning objectives.
format	article
author	Kathryn E. Gunn Christine Seitz McCauslin Jennifer Staiger Dana M. Pirone
author_facet	Kathryn E. Gunn Christine Seitz McCauslin Jennifer Staiger Dana M. Pirone
author_sort	Kathryn E. Gunn
title	Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression
title_short	Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression
title_full	Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression
title_fullStr	Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression
title_full_unstemmed	Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression
title_sort	inquiry-based learning: inflammation as a model to teach molecular techniques for assessing gene expression
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/96cd79083bed43f29288c3f30c089e9f
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Inquiry-Based Learning: Inflammation as a Model to Teach Molecular Techniques for Assessing Gene Expression

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