Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials

Lucas Amaral-Machado,1 Wógenes N Oliveira,1 Manoela Torres-Rêgo,2 Allanny A Furtado,3 Éverton N Alencar,1 Matheus F Fernandes-Pedrosa,3 Hugo AO Rocha,1 Eryvaldo Sócrates Tabosa Egito1,3 1Graduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazi...

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Autores principales:	Amaral-Machado L, Oliveira WN, Torres-Rêgo M, Furtado AA, Alencar EN, Fernandes-Pedrosa MF, Rocha HAO, Egito EST
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	polyunsaturated fatty acids bullfrog oil nanocapsules anti-inflammatory activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/96ce600aad9a4c548c225a1b27d18d95
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spelling	oai:doaj.org-article:96ce600aad9a4c548c225a1b27d18d952021-12-02T17:25:13ZAnti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials1178-2013https://doaj.org/article/96ce600aad9a4c548c225a1b27d18d952021-11-01T00:00:00Zhttps://www.dovepress.com/anti-inflammatory-activity-of-bullfrog-oil-polymeric-nanocapsules-from-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Lucas Amaral-Machado,1 Wógenes N Oliveira,1 Manoela Torres-Rêgo,2 Allanny A Furtado,3 Éverton N Alencar,1 Matheus F Fernandes-Pedrosa,3 Hugo AO Rocha,1 Eryvaldo Sócrates Tabosa Egito1,3 1Graduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil; 2Chemistry Institute, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil; 3Graduate Program of Pharmaceutical Sciences, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilCorrespondence: Eryvaldo Sócrates Tabosa EgitoFederal University of Rio Grande do Norte, Rua Jaguarari, 4985 – Apt. 1603D - 59054-500, Natal, Rio Grande do Norte, BrazilTel +55-84-994-31-88-16Fax +55-84-3342-9817; +55-84-3342-9817-9808Email socratesegito@gmail.comBackground: Although bullfrog oil (BFO) exerts anti-inflammatory effects, it has undesirable properties limiting its use.Methodology: BFO nanocapsules (BFONc) were produced through nanoprecipitation, and their physicochemical and morphological properties were characterized. To evaluate the biocompatibility of the formulation, a mitochondrial activity evaluation assay was conducted, and cell uptake was assessed. The in vitro anti-inflammatory activity was evaluated by measuring reactive oxygen species (ROS), nitric oxide (NO), type-6 interleukin (IL-6), and tumor necrosis factor (TNF) levels. The in vivo anti-inflammatory effect was assessed by quantifying myeloperoxidase (MPO) levels using the carrageenan-induced paw edema model.Results: BFONc showed a particle size of 233 ± 22 nm, a polydispersity index of 0.17 ± 0.03, and a zeta potential of − 34 ± 2.6mV. BFONc revealed remarkable biocompatibility and did not induce changes in cell morphology. Furthermore, BFONc decreased ROS levels by 81 ± 4%; however, NO level increased by 72 ± 18%. TNF and IL-6 levels were reduced by approximately 10% and 90%, respectively. Significant in vivo anti-inflammatory activity was observed compared to dexamethasone. MPO levels were reduced up to 2 MPOs/mg.Conclusion: Taken together, the results pointed out the remarkable biocompatibility and anti-inflammatory effects of BFONc.Keywords: polyunsaturated fatty acids, bullfrog oil, nanocapsules, anti-inflammatory activityAmaral-Machado LOliveira WNTorres-Rêgo MFurtado AAAlencar ENFernandes-Pedrosa MFRocha HAOEgito ESTDove Medical Pressarticlepolyunsaturated fatty acidsbullfrog oilnanocapsulesanti-inflammatory activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 7353-7367 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyunsaturated fatty acids bullfrog oil nanocapsules anti-inflammatory activity Medicine (General) R5-920
spellingShingle	polyunsaturated fatty acids bullfrog oil nanocapsules anti-inflammatory activity Medicine (General) R5-920 Amaral-Machado L Oliveira WN Torres-Rêgo M Furtado AA Alencar EN Fernandes-Pedrosa MF Rocha HAO Egito EST Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials
description	Lucas Amaral-Machado,1 Wógenes N Oliveira,1 Manoela Torres-Rêgo,2 Allanny A Furtado,3 Éverton N Alencar,1 Matheus F Fernandes-Pedrosa,3 Hugo AO Rocha,1 Eryvaldo Sócrates Tabosa Egito1,3 1Graduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil; 2Chemistry Institute, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil; 3Graduate Program of Pharmaceutical Sciences, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilCorrespondence: Eryvaldo Sócrates Tabosa EgitoFederal University of Rio Grande do Norte, Rua Jaguarari, 4985 – Apt. 1603D - 59054-500, Natal, Rio Grande do Norte, BrazilTel +55-84-994-31-88-16Fax +55-84-3342-9817; +55-84-3342-9817-9808Email socratesegito@gmail.comBackground: Although bullfrog oil (BFO) exerts anti-inflammatory effects, it has undesirable properties limiting its use.Methodology: BFO nanocapsules (BFONc) were produced through nanoprecipitation, and their physicochemical and morphological properties were characterized. To evaluate the biocompatibility of the formulation, a mitochondrial activity evaluation assay was conducted, and cell uptake was assessed. The in vitro anti-inflammatory activity was evaluated by measuring reactive oxygen species (ROS), nitric oxide (NO), type-6 interleukin (IL-6), and tumor necrosis factor (TNF) levels. The in vivo anti-inflammatory effect was assessed by quantifying myeloperoxidase (MPO) levels using the carrageenan-induced paw edema model.Results: BFONc showed a particle size of 233 ± 22 nm, a polydispersity index of 0.17 ± 0.03, and a zeta potential of − 34 ± 2.6mV. BFONc revealed remarkable biocompatibility and did not induce changes in cell morphology. Furthermore, BFONc decreased ROS levels by 81 ± 4%; however, NO level increased by 72 ± 18%. TNF and IL-6 levels were reduced by approximately 10% and 90%, respectively. Significant in vivo anti-inflammatory activity was observed compared to dexamethasone. MPO levels were reduced up to 2 MPOs/mg.Conclusion: Taken together, the results pointed out the remarkable biocompatibility and anti-inflammatory effects of BFONc.Keywords: polyunsaturated fatty acids, bullfrog oil, nanocapsules, anti-inflammatory activity
format	article
author	Amaral-Machado L Oliveira WN Torres-Rêgo M Furtado AA Alencar EN Fernandes-Pedrosa MF Rocha HAO Egito EST
author_facet	Amaral-Machado L Oliveira WN Torres-Rêgo M Furtado AA Alencar EN Fernandes-Pedrosa MF Rocha HAO Egito EST
author_sort	Amaral-Machado L
title	Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials
title_short	Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials
title_full	Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials
title_fullStr	Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials
title_full_unstemmed	Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials
title_sort	anti-inflammatory activity of bullfrog oil polymeric nanocapsules: from the design to preclinical trials
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/96ce600aad9a4c548c225a1b27d18d95
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Anti-Inflammatory Activity of Bullfrog Oil Polymeric Nanocapsules: From the Design to Preclinical Trials

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