Quantum dot-induced viral capsid assembling in dissociation buffer

Ding Gao,1,2 Zhi-Ping Zhang,1 Feng Li,3 Dong Men,1 Jiao-Yu Deng,1 Hong-Ping Wei,1 Xian-En Zhang,1 Zong-Qiang Cui1 1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 2Graduate University of Chinese Academy of Sciences, Beijing, 3Division of Nanobiomedi...

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Autores principales:	Gao D, Zhang ZP, Li F, Men D, Deng JY, Wei HP, Zhang XE, Cui ZQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/96d18579279540e1aa1f0e0a3f08e31e
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spelling	oai:doaj.org-article:96d18579279540e1aa1f0e0a3f08e31e2021-12-02T02:01:52ZQuantum dot-induced viral capsid assembling in dissociation buffer1176-91141178-2013https://doaj.org/article/96d18579279540e1aa1f0e0a3f08e31e2013-06-01T00:00:00Zhttp://www.dovepress.com/quantum-dot-induced-viral-capsid-assembling-in-dissociation-buffer-a13258https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ding Gao,1,2 Zhi-Ping Zhang,1 Feng Li,3 Dong Men,1 Jiao-Yu Deng,1 Hong-Ping Wei,1 Xian-En Zhang,1 Zong-Qiang Cui1 1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 2Graduate University of Chinese Academy of Sciences, Beijing, 3Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, People's Republic of China Abstract: Viruses encapsulating inorganic nanoparticles are a novel type of nanostructure with applications in biomedicine and biosensors. However, the encapsulation and assembly mechanisms of these hybridized virus-based nanoparticles (VNPs) are still unknown. In this article, it was found that quantum dots (QDs) can induce simian virus 40 (SV40) capsid assembly in dissociation buffer, where viral capsids should be disassembled. The analysis of the transmission electron microscope, dynamic light scattering, sucrose density gradient centrifugation, and cryo-electron microscopy single particle reconstruction experimental results showed that the SV40 major capsid protein 1 (VP1) can be assembled into ≈25 nm capsids in the dissociation buffer when QDs are present and that the QDs are encapsulated in the SV40 capsids. Moreover, it was determined that there is a strong affinity between QDs and the SV40 VP1 proteins (KD = 2.19E-10 M), which should play an important role in QD encapsulation in the SV40 viral capsids. This study provides a new understanding of the assembly mechanism of SV40 virus-based nanoparticles with QDs, which may help in the design and construction of other similar virus-based nanoparticles. Keywords: quantum dots, simian virus 40, self-assembly, encapsulation, virus-based nanoparticlesGao DZhang ZPLi FMen DDeng JYWei HPZhang XECui ZQDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2119-2128 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Gao D Zhang ZP Li F Men D Deng JY Wei HP Zhang XE Cui ZQ Quantum dot-induced viral capsid assembling in dissociation buffer
description	Ding Gao,1,2 Zhi-Ping Zhang,1 Feng Li,3 Dong Men,1 Jiao-Yu Deng,1 Hong-Ping Wei,1 Xian-En Zhang,1 Zong-Qiang Cui1 1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 2Graduate University of Chinese Academy of Sciences, Beijing, 3Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, People's Republic of China Abstract: Viruses encapsulating inorganic nanoparticles are a novel type of nanostructure with applications in biomedicine and biosensors. However, the encapsulation and assembly mechanisms of these hybridized virus-based nanoparticles (VNPs) are still unknown. In this article, it was found that quantum dots (QDs) can induce simian virus 40 (SV40) capsid assembly in dissociation buffer, where viral capsids should be disassembled. The analysis of the transmission electron microscope, dynamic light scattering, sucrose density gradient centrifugation, and cryo-electron microscopy single particle reconstruction experimental results showed that the SV40 major capsid protein 1 (VP1) can be assembled into ≈25 nm capsids in the dissociation buffer when QDs are present and that the QDs are encapsulated in the SV40 capsids. Moreover, it was determined that there is a strong affinity between QDs and the SV40 VP1 proteins (KD = 2.19E-10 M), which should play an important role in QD encapsulation in the SV40 viral capsids. This study provides a new understanding of the assembly mechanism of SV40 virus-based nanoparticles with QDs, which may help in the design and construction of other similar virus-based nanoparticles. Keywords: quantum dots, simian virus 40, self-assembly, encapsulation, virus-based nanoparticles
format	article
author	Gao D Zhang ZP Li F Men D Deng JY Wei HP Zhang XE Cui ZQ
author_facet	Gao D Zhang ZP Li F Men D Deng JY Wei HP Zhang XE Cui ZQ
author_sort	Gao D
title	Quantum dot-induced viral capsid assembling in dissociation buffer
title_short	Quantum dot-induced viral capsid assembling in dissociation buffer
title_full	Quantum dot-induced viral capsid assembling in dissociation buffer
title_fullStr	Quantum dot-induced viral capsid assembling in dissociation buffer
title_full_unstemmed	Quantum dot-induced viral capsid assembling in dissociation buffer
title_sort	quantum dot-induced viral capsid assembling in dissociation buffer
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/96d18579279540e1aa1f0e0a3f08e31e
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Quantum dot-induced viral capsid assembling in dissociation buffer

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