Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase

The article considers the production of red blood cell “ghosts” and their application as a model for studying Na+, K+ - ATPase. Various variants of liposomes are used as test objects for studying ion transport, but the best model of biological membranes for studying ion transport and ATPase pumps ar...

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Autores principales:	Mosyagin Vladimir, Ryzhkova Galina, Belyaev Alexey, Sidorenko Semyon
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/96d295900cbc45e5bfcc11452b7f6faf
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id	oai:doaj.org-article:96d295900cbc45e5bfcc11452b7f6faf
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spelling	oai:doaj.org-article:96d295900cbc45e5bfcc11452b7f6faf2021-11-08T15:17:24ZObtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase2117-445810.1051/bioconf/20213700059https://doaj.org/article/96d295900cbc45e5bfcc11452b7f6faf2021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/09/bioconf_fies2021_00059.pdfhttps://doaj.org/toc/2117-4458The article considers the production of red blood cell “ghosts” and their application as a model for studying Na+, K+ - ATPase. Various variants of liposomes are used as test objects for studying ion transport, but the best model of biological membranes for studying ion transport and ATPase pumps are the «ghosts» of red blood cells. The activity of blood Na+/K+ - ATPase is determined mainly in the suspension of red blood cell “ghosts”. This is due to the need to remove the interfering compound – hemoglobin - and obtain “ghosts” that are necessary for accurate measurement of the activity of the enzyme. In this scientific work, studies were conducted to determine the concentrations of Na+ and K+ ions in the blood and red blood cells of cows and calves of various ages by atomic absorption and atomic emission spectroscopy. The values of these concentrations of Na+ and K+ ions were used to obtain red blood cell “ghosts”. “Ghosts” of red blood cells with a set concentration of sodium and potassium ions were obtained by the method of J. T. Dodge, in the modification of Zhumadilov Zh. Sh. and Gening T. P. and in our modification. Studies of the concentration of Na+ and K+ ions in the obtained «ghosts» of red blood cells showed their full compliance with the calculated values.Mosyagin VladimirRyzhkova GalinaBelyaev AlexeySidorenko SemyonEDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 37, p 00059 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Mosyagin Vladimir Ryzhkova Galina Belyaev Alexey Sidorenko Semyon Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase
description	The article considers the production of red blood cell “ghosts” and their application as a model for studying Na+, K+ - ATPase. Various variants of liposomes are used as test objects for studying ion transport, but the best model of biological membranes for studying ion transport and ATPase pumps are the «ghosts» of red blood cells. The activity of blood Na+/K+ - ATPase is determined mainly in the suspension of red blood cell “ghosts”. This is due to the need to remove the interfering compound – hemoglobin - and obtain “ghosts” that are necessary for accurate measurement of the activity of the enzyme. In this scientific work, studies were conducted to determine the concentrations of Na+ and K+ ions in the blood and red blood cells of cows and calves of various ages by atomic absorption and atomic emission spectroscopy. The values of these concentrations of Na+ and K+ ions were used to obtain red blood cell “ghosts”. “Ghosts” of red blood cells with a set concentration of sodium and potassium ions were obtained by the method of J. T. Dodge, in the modification of Zhumadilov Zh. Sh. and Gening T. P. and in our modification. Studies of the concentration of Na+ and K+ ions in the obtained «ghosts» of red blood cells showed their full compliance with the calculated values.
format	article
author	Mosyagin Vladimir Ryzhkova Galina Belyaev Alexey Sidorenko Semyon
author_facet	Mosyagin Vladimir Ryzhkova Galina Belyaev Alexey Sidorenko Semyon
author_sort	Mosyagin Vladimir
title	Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase
title_short	Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase
title_full	Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase
title_fullStr	Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase
title_full_unstemmed	Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase
title_sort	obtaining “ghosts” of red blood cells with a set ion concentration as models for studying na+, k+ - atpase
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/96d295900cbc45e5bfcc11452b7f6faf
work_keys_str_mv	AT mosyaginvladimir obtainingghostsofredbloodcellswithasetionconcentrationasmodelsforstudyingnakatpase AT ryzhkovagalina obtainingghostsofredbloodcellswithasetionconcentrationasmodelsforstudyingnakatpase AT belyaevalexey obtainingghostsofredbloodcellswithasetionconcentrationasmodelsforstudyingnakatpase AT sidorenkosemyon obtainingghostsofredbloodcellswithasetionconcentrationasmodelsforstudyingnakatpase
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Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase

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