Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.

<h4>Background</h4>The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity...

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Autores principales:	Till Jasper Meyer, Elena Gerhard-Hartmann, Nina Lodes, Agmal Scherzad, Rudolf Hagen, Maria Steinke, Stephan Hackenberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96d9b923cab44b6b8c1c955f1cc99624
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spelling	oai:doaj.org-article:96d9b923cab44b6b8c1c955f1cc996242021-12-02T20:14:33ZPilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.1932-620310.1371/journal.pone.0257470https://doaj.org/article/96d9b923cab44b6b8c1c955f1cc996242021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257470https://doaj.org/toc/1932-6203<h4>Background</h4>The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT.<h4>Methods</h4>Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software.<h4>Results</h4>The Raman spectra detected in ACC samples were mostly assigned to nucleic acids, lipids, and amides. In a principal component-based linear discriminant analysis (LDA) 18 of 20 tumor samples were classified correctly.<h4>Conclusion</h4>In this proof of concept study, we show that a reliable SGT diagnosis based on LDA algorithm appears possible, despite variations in the entity-specific mean spectra. However, a standardized workflow for tissue sample preparation, measurement setup, and chemometric algorithms is essential to get reliable results.Till Jasper MeyerElena Gerhard-HartmannNina LodesAgmal ScherzadRudolf HagenMaria SteinkeStephan HackenbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257470 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Till Jasper Meyer Elena Gerhard-Hartmann Nina Lodes Agmal Scherzad Rudolf Hagen Maria Steinke Stephan Hackenberg Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.
description	<h4>Background</h4>The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT.<h4>Methods</h4>Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software.<h4>Results</h4>The Raman spectra detected in ACC samples were mostly assigned to nucleic acids, lipids, and amides. In a principal component-based linear discriminant analysis (LDA) 18 of 20 tumor samples were classified correctly.<h4>Conclusion</h4>In this proof of concept study, we show that a reliable SGT diagnosis based on LDA algorithm appears possible, despite variations in the entity-specific mean spectra. However, a standardized workflow for tissue sample preparation, measurement setup, and chemometric algorithms is essential to get reliable results.
format	article
author	Till Jasper Meyer Elena Gerhard-Hartmann Nina Lodes Agmal Scherzad Rudolf Hagen Maria Steinke Stephan Hackenberg
author_facet	Till Jasper Meyer Elena Gerhard-Hartmann Nina Lodes Agmal Scherzad Rudolf Hagen Maria Steinke Stephan Hackenberg
author_sort	Till Jasper Meyer
title	Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.
title_short	Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.
title_full	Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.
title_fullStr	Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.
title_full_unstemmed	Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.
title_sort	pilot study on the value of raman spectroscopy in the entity assignment of salivary gland tumors.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/96d9b923cab44b6b8c1c955f1cc99624
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Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors.

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