Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii

Abstract Contamination by mercury (Hg) is a worldwide concern because of Hg toxicity and biomagnification in aquatic food webs. Nevertheless, bioavailability and cellular toxicity pathways of inorganic (IHg) and methyl-Hg (MeHg) remain poorly understood. We analyzed the uptake, transcriptomic, and p...

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Autores principales:	Rebecca Beauvais-Flück, Vera I. Slaveykova, Claudia Cosio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96e0f9cea2614f1892ababaa8161a680
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spelling	oai:doaj.org-article:96e0f9cea2614f1892ababaa8161a6802021-12-02T16:06:08ZCellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii10.1038/s41598-017-08515-82045-2322https://doaj.org/article/96e0f9cea2614f1892ababaa8161a6802017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08515-8https://doaj.org/toc/2045-2322Abstract Contamination by mercury (Hg) is a worldwide concern because of Hg toxicity and biomagnification in aquatic food webs. Nevertheless, bioavailability and cellular toxicity pathways of inorganic (IHg) and methyl-Hg (MeHg) remain poorly understood. We analyzed the uptake, transcriptomic, and physiological responses in the microalga Chlamydomonas reinhardtii exposed to IHg or MeHg. Bioavailability of MeHg was up to 27× higher than for IHg. Genes involved in cell processes, energy metabolism and transport were dysregulated by both Hg species. Physiological analysis revealed an impact on photosynthesis and reduction–oxidation reaction metabolism. Nevertheless, MeHg dysregulated a larger number of genes and with a stronger fold-change than IHg at equivalent intracellular concentration. Analysis of the perturbations of the cell’s functions helped to derive a detailed mechanistic understanding of differences in cellular handling of IHg and MeHg resulting in MeHg having a stronger impact. This knowledge is central for the prediction of impact of toxicants on organisms.Rebecca Beauvais-FlückVera I. SlaveykovaClaudia CosioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rebecca Beauvais-Flück Vera I. Slaveykova Claudia Cosio Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
description	Abstract Contamination by mercury (Hg) is a worldwide concern because of Hg toxicity and biomagnification in aquatic food webs. Nevertheless, bioavailability and cellular toxicity pathways of inorganic (IHg) and methyl-Hg (MeHg) remain poorly understood. We analyzed the uptake, transcriptomic, and physiological responses in the microalga Chlamydomonas reinhardtii exposed to IHg or MeHg. Bioavailability of MeHg was up to 27× higher than for IHg. Genes involved in cell processes, energy metabolism and transport were dysregulated by both Hg species. Physiological analysis revealed an impact on photosynthesis and reduction–oxidation reaction metabolism. Nevertheless, MeHg dysregulated a larger number of genes and with a stronger fold-change than IHg at equivalent intracellular concentration. Analysis of the perturbations of the cell’s functions helped to derive a detailed mechanistic understanding of differences in cellular handling of IHg and MeHg resulting in MeHg having a stronger impact. This knowledge is central for the prediction of impact of toxicants on organisms.
format	article
author	Rebecca Beauvais-Flück Vera I. Slaveykova Claudia Cosio
author_facet	Rebecca Beauvais-Flück Vera I. Slaveykova Claudia Cosio
author_sort	Rebecca Beauvais-Flück
title	Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
title_short	Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
title_full	Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
title_fullStr	Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
title_full_unstemmed	Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
title_sort	cellular toxicity pathways of inorganic and methyl mercury in the green microalga chlamydomonas reinhardtii
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/96e0f9cea2614f1892ababaa8161a680
work_keys_str_mv	AT rebeccabeauvaisfluck cellulartoxicitypathwaysofinorganicandmethylmercuryinthegreenmicroalgachlamydomonasreinhardtii AT veraislaveykova cellulartoxicitypathwaysofinorganicandmethylmercuryinthegreenmicroalgachlamydomonasreinhardtii AT claudiacosio cellulartoxicitypathwaysofinorganicandmethylmercuryinthegreenmicroalgachlamydomonasreinhardtii
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Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii

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