Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4

Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, β-galactosidase and...

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Autores principales:	Pin Chen, Xiaoqian Chen, Wei Yu, Bo Zhou, Lihua Liu, Yuzhuo Yang, Peng Du, Libo Liu, Chun Li
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Ciprofloxacin Lactobacillus plantarum DNZ-4 Key enzymes Metabolism Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/96e156a78ebb48ecab15d5b736f56ccc
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spelling	oai:doaj.org-article:96e156a78ebb48ecab15d5b736f56ccc2021-11-28T04:31:53ZCiprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-42213-453010.1016/j.fshw.2021.11.007https://doaj.org/article/96e156a78ebb48ecab15d5b736f56ccc2022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213453021001038https://doaj.org/toc/2213-4530Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, β-galactosidase and Na+, K+-ATPase after 1/2 minimum bacteriostatic concentration (MIC) CIP treatment were significantly decreased (P < 0.01). Gas chromatography-mass spectrometry was used to analysis the changes of main metabolites in the cells and principal component analysis and partial least square model were constructed. The results indicated that CIP could cause changes in intracellular fatty acids, carbohydrates and amino acids, and the mechanism of amino acid metabolism under CIP stress was significantly inhibited. L. plantarum DNZ-4 made stress response to CIP by regulating the ratio of saturated fatty acids and unsaturated fats. This experiment revealed the changes of growth and metabolism mechanism of L. plantarum DNZ-4 under CIP stress, which help to provide technical means for the development of effective probiotics preparation products.Pin ChenXiaoqian ChenWei YuBo ZhouLihua LiuYuzhuo YangPeng DuLibo LiuChun LiKeAi Communications Co., Ltd.articleCiprofloxacinLactobacillus plantarum DNZ-4Key enzymesMetabolismNutrition. Foods and food supplyTX341-641ENFood Science and Human Wellness, Vol 11, Iss 2, Pp 332-340 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ciprofloxacin Lactobacillus plantarum DNZ-4 Key enzymes Metabolism Nutrition. Foods and food supply TX341-641
spellingShingle	Ciprofloxacin Lactobacillus plantarum DNZ-4 Key enzymes Metabolism Nutrition. Foods and food supply TX341-641 Pin Chen Xiaoqian Chen Wei Yu Bo Zhou Lihua Liu Yuzhuo Yang Peng Du Libo Liu Chun Li Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
description	Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, β-galactosidase and Na+, K+-ATPase after 1/2 minimum bacteriostatic concentration (MIC) CIP treatment were significantly decreased (P < 0.01). Gas chromatography-mass spectrometry was used to analysis the changes of main metabolites in the cells and principal component analysis and partial least square model were constructed. The results indicated that CIP could cause changes in intracellular fatty acids, carbohydrates and amino acids, and the mechanism of amino acid metabolism under CIP stress was significantly inhibited. L. plantarum DNZ-4 made stress response to CIP by regulating the ratio of saturated fatty acids and unsaturated fats. This experiment revealed the changes of growth and metabolism mechanism of L. plantarum DNZ-4 under CIP stress, which help to provide technical means for the development of effective probiotics preparation products.
format	article
author	Pin Chen Xiaoqian Chen Wei Yu Bo Zhou Lihua Liu Yuzhuo Yang Peng Du Libo Liu Chun Li
author_facet	Pin Chen Xiaoqian Chen Wei Yu Bo Zhou Lihua Liu Yuzhuo Yang Peng Du Libo Liu Chun Li
author_sort	Pin Chen
title	Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
title_short	Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
title_full	Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
title_fullStr	Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
title_full_unstemmed	Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
title_sort	ciprofloxacin stress changes key enzymes and intracellular metabolites of lactobacillus plantarum dnz-4
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/96e156a78ebb48ecab15d5b736f56ccc
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Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4

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