Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis

WooHyung Jang,1 Hee-Seon Kim,1 Khurshed Alam,2 Min-Kyung Ji,3 Hoon-Sung Cho,2 Hyun-Pil Lim1 1Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Korea; 2Department of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, Korea; 3Optoelec...

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Autores principales:	Jang W, Kim HS, Alam K, Ji MK, Cho HS, Lim HP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	graphene oxide (go) zirconia implant biofilm formation osteoblast non-thermal atmospheric pressure plasma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/96eab421e9f74f59b5b7af0726236991
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spelling	oai:doaj.org-article:96eab421e9f74f59b5b7af07262369912021-12-02T16:39:58ZDirect-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis1178-2013https://doaj.org/article/96eab421e9f74f59b5b7af07262369912021-08-01T00:00:00Zhttps://www.dovepress.com/direct-deposited-graphene-oxide-on-dental-implants-for-antimicrobial-a-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013WooHyung Jang,1 Hee-Seon Kim,1 Khurshed Alam,2 Min-Kyung Ji,3 Hoon-Sung Cho,2 Hyun-Pil Lim1 1Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Korea; 2Department of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, Korea; 3Optoelectronics Convergence Research Center, Chonnam National University, Gwangju, 61186, KoreaCorrespondence: Hyun-Pil LimDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, 61186, KoreaTel +82-10-2645-7528Fax +82-62-530-5577Email mcnihil@jnu.ac.krHoon-Sung ChoDepartment of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, KoreaTel/Fax +82-62-530-1717Email cho.hoonsung@jnu.ac.krObjective: To determine the effects of graphene oxide (GO) deposition (on a zirconia surface) on bacterial adhesion and osteoblast activation.Methods: An atmospheric pressure plasma generator (PGS-300) was used to coat Ar/CH4 mixed gas onto zirconia specimens (15-mm diameter × 2.5-mm thick disks) at a rate of 10 L/min and 240 V. Zirconia specimens were divided into two groups: uncoated (control; Zr) group and GO-coated (Zr-GO) group. Surface characteristics and element structures of each specimen were evaluated by field emission scanning electron microscope (FE-SEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and contact angle. Additionally, crystal violet staining was performed to assess the adhesion of Streptococcus mutans. WST-8 and ALP (Alkaline phosphatase) assays were conducted to evaluate MC3T3-E1 osteoblast adhesion, proliferation, and differentiation. Statistical analysis was calculated by the Mann–Whitney U-test.Results: FE–SEM and Raman spectroscopy demonstrated effective GO deposition on the zirconia surface in Zr-GO. The attachment and biofilm formation of S. mutans was significantly reduced in Zr-GO compared with that of Zr (P < 0.05). While no significant differences in cell attachment of MC3T3-1 were observed, both proliferation and differentiation were increased in Zr-GO as compared with that of Zr (P < 0.05).Significance: GO-coated zirconia inhibited the attachment of S. mutans and stimulated proliferation and differentiation of osteoblasts. Therefore, GO-coated zirconia can prevent peri-implantitis by inhibiting bacterial adhesion. Moreover, its osteogenic ability can increase bone adhesion and success rate of implants.Keywords: graphene oxide, GO, zirconia implant, biofilm formation, osteoblast, non-thermal atmospheric pressure plasmaJang WKim HSAlam KJi MKCho HSLim HPDove Medical Pressarticlegraphene oxide (go)zirconia implantbiofilm formationosteoblastnon-thermal atmospheric pressure plasmaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 5745-5754 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	graphene oxide (go) zirconia implant biofilm formation osteoblast non-thermal atmospheric pressure plasma Medicine (General) R5-920
spellingShingle	graphene oxide (go) zirconia implant biofilm formation osteoblast non-thermal atmospheric pressure plasma Medicine (General) R5-920 Jang W Kim HS Alam K Ji MK Cho HS Lim HP Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis
description	WooHyung Jang,1 Hee-Seon Kim,1 Khurshed Alam,2 Min-Kyung Ji,3 Hoon-Sung Cho,2 Hyun-Pil Lim1 1Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Korea; 2Department of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, Korea; 3Optoelectronics Convergence Research Center, Chonnam National University, Gwangju, 61186, KoreaCorrespondence: Hyun-Pil LimDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, 61186, KoreaTel +82-10-2645-7528Fax +82-62-530-5577Email mcnihil@jnu.ac.krHoon-Sung ChoDepartment of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, KoreaTel/Fax +82-62-530-1717Email cho.hoonsung@jnu.ac.krObjective: To determine the effects of graphene oxide (GO) deposition (on a zirconia surface) on bacterial adhesion and osteoblast activation.Methods: An atmospheric pressure plasma generator (PGS-300) was used to coat Ar/CH4 mixed gas onto zirconia specimens (15-mm diameter × 2.5-mm thick disks) at a rate of 10 L/min and 240 V. Zirconia specimens were divided into two groups: uncoated (control; Zr) group and GO-coated (Zr-GO) group. Surface characteristics and element structures of each specimen were evaluated by field emission scanning electron microscope (FE-SEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and contact angle. Additionally, crystal violet staining was performed to assess the adhesion of Streptococcus mutans. WST-8 and ALP (Alkaline phosphatase) assays were conducted to evaluate MC3T3-E1 osteoblast adhesion, proliferation, and differentiation. Statistical analysis was calculated by the Mann–Whitney U-test.Results: FE–SEM and Raman spectroscopy demonstrated effective GO deposition on the zirconia surface in Zr-GO. The attachment and biofilm formation of S. mutans was significantly reduced in Zr-GO compared with that of Zr (P < 0.05). While no significant differences in cell attachment of MC3T3-1 were observed, both proliferation and differentiation were increased in Zr-GO as compared with that of Zr (P < 0.05).Significance: GO-coated zirconia inhibited the attachment of S. mutans and stimulated proliferation and differentiation of osteoblasts. Therefore, GO-coated zirconia can prevent peri-implantitis by inhibiting bacterial adhesion. Moreover, its osteogenic ability can increase bone adhesion and success rate of implants.Keywords: graphene oxide, GO, zirconia implant, biofilm formation, osteoblast, non-thermal atmospheric pressure plasma
format	article
author	Jang W Kim HS Alam K Ji MK Cho HS Lim HP
author_facet	Jang W Kim HS Alam K Ji MK Cho HS Lim HP
author_sort	Jang W
title	Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis
title_short	Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis
title_full	Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis
title_fullStr	Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis
title_full_unstemmed	Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis
title_sort	direct-deposited graphene oxide on dental implants for antimicrobial activities and osteogenesis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/96eab421e9f74f59b5b7af0726236991
work_keys_str_mv	AT jangw directdepositedgrapheneoxideondentalimplantsforantimicrobialactivitiesandosteogenesis AT kimhs directdepositedgrapheneoxideondentalimplantsforantimicrobialactivitiesandosteogenesis AT alamk directdepositedgrapheneoxideondentalimplantsforantimicrobialactivitiesandosteogenesis AT jimk directdepositedgrapheneoxideondentalimplantsforantimicrobialactivitiesandosteogenesis AT chohs directdepositedgrapheneoxideondentalimplantsforantimicrobialactivitiesandosteogenesis AT limhp directdepositedgrapheneoxideondentalimplantsforantimicrobialactivitiesandosteogenesis
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Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis

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